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Council Directive 2009/156/ECDangos y teitl llawn

Council Directive 2009/156/EC of 30 November 2009 on animal health conditions governing the movement and importation from third countries of equidae (codified version) (Text with EEA relevance)

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  1. Introductory Text

  2. CHAPTER I GENERAL PROVISIONS

    1. Article 1.This Directive lays down animal health conditions for the movement...

    2. Article 2.For the purposes of this Directive the following definitions shall...

  3. CHAPTER II RULES FOR THE MOVEMENT OF EQUIDAE BETWEEN MEMBER STATES

    1. Article 3.Member States shall authorise the movement of registered equidae in...

    2. Article 4.(1) Equidae must show no clinical sign of disease at...

    3. Article 5.(1) A Member State which is not free from African...

    4. Article 6.Member States which implement an alternative control system providing guarantees...

    5. Article 7.(1) Equidae must be transported, as soon as possible, from...

    6. Article 8.(1) Member States shall ensure that: (a) registered equidae which...

    7. Article 9.The rules laid down in Directive 90/425/EEC shall apply in...

    8. Article 10.Veterinary experts from the Commission may, to the extent necessary...

  4. CHAPTER III RULES FOR IMPORTATION OF EQUIDAE FROM THIRD COUNTRIES

    1. Article 11.Equidae imported into the Community must satisfy the conditions laid...

    2. Article 12.(1) The importation of equidae into the Community shall only...

    3. Article 13.(1) The equidae must come from third countries which:

    4. Article 14.Before the day of loading for transportation to the Member...

    5. Article 15.Importation of equidae from the territory of a third country...

    6. Article 16.(1) The equidae must be identified in accordance with Article...

    7. Article 17.(1) Immediately upon arrival in the Member State of destination,...

    8. Article 18.Checks shall be carried out on the spot by veterinary...

    9. Article 19.In accordance with the procedure referred to in Article 21(2):...

  5. CHAPTER IV FINAL PROVISIONS

    1. Article 20.Annexes I to IV shall be amended in accordance with...

    2. Article 21.(1) The Commission shall be assisted by the Standing Committee...

    3. Article 22.Directive 90/426/EEC, as amended by the acts listed in Annex...

    4. Article 23.This Directive shall enter into force on the 20th day...

    5. Article 24.This Directive is addressed to the Member States.

  6. Signature

    1. ANNEX I

      COMPULSORILY NOTIFIABLE DISEASES

      1. The following diseases are compulsorily notifiable:

      2. Dourine Glanders Equine encephalomyelitis (of all types, including VEE) Infectious...

    2. ANNEX II

      1. MODEL HEALTH ATTESTATION

    3. ANNEX III

      1. MODEL HEALTH CERTIFICATE For trade between Member States EQUIDAE ANNEX IV...For trade between Member States EQUIDAE ANNEX IV AFRICAN HORSE...EQUIDAE ANNEX IV AFRICAN HORSE SICKNESS DIAGNOSIS Reagents for the...

    4. ANNEX IV

      1. AFRICAN HORSE SICKNESS DIAGNOSIS

        1. 1. COMPETITIVE ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE...

          1. 1.1. Test procedure

            1. 1.1.1. Preparation of plates

              1. 1.1.1.1. Coat ELISA plates with AHSV antigen extracted from infected cell...

              2. 1.1.1.2. Wash plates three times by flooding and emptying the wells...

            2. 1.1.2. Control wells

              1. 1.1.2.1. Titrate the positive control sera in a twofold dilution series,...

              2. 1.1.2.2. Add 50 μl of the negative control serum at a...

              3. 1.1.2.3. Add 100 μl/well of blocking buffer to wells C and...

              4. 1.1.2.4. Add 50 μl of blocking buffer to wells E, F,...

            3. 1.1.3. Spot test method

              1. 1.1.3.1. Add a 1 in 5 dilution of each test serum...

            4. 1.1.4. Serum titration method

              1. 1.1.4.1. Prepare a twofold dilution series of each test sample (1...

            5. 1.1.5. Add 50 μl of guinea pig antisera, pre-diluted in blocking...

              1. 1.1.5.1. Incubate for 1 hour at 37 °C on an orbital shaker....

              2. 1.1.5.2. Wash plates three times and blot dry as before.

              3. 1.1.5.3. Add 50 μl of rabbit anti-guinea-pig horseradish peroxidase (HRP) conjugate...

              4. 1.1.5.4. Incubate for 1 hour at 37 °C on an orbital shaker....

              5. 1.1.5.5. Wash plates three times and blot dry as before.

            6. 1.1.6. Chromogen

            7. 1.1.7. Reading

          2. 1.2. Expression of results

            1. 1.2.1. Using a software package print out the optical density (OD)...

            2. 1.2.2. The diagnostic threshold (cut-off value) for test sera is 50 %...

        2. 2. INDIRECT ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE...

          1. 2.1. Test procedure

            1. 2.1.1. Solid phase

              1. 2.1.1.1. ELISA plates are coated with recombinant AHSV-4 VP7 diluted in...

              2. 2.1.1.2. Wash the plates five times with distilled water containing 0,01 %...

              3. 2.1.1.3. Block the plates with phosphate buffered saline (PBS) + 5 %...

              4. 2.1.1.4. Remove the blocking solution and gently tap the plates onto...

            2. 2.1.2. Test samples

              1. 2.1.2.1. Serum samples to be tested, and positive and negative control...

              2. 2.1.2.2. Wash the plates as described in step 2.1.1.2.

            3. 2.1.3. Conjugate

              1. 2.1.3.1. Dispense 100 μl/well of horseradish-peroxidase (HRP) -conjugated anti-horse gamma-globulin diluted...

              2. 2.1.3.2. Wash the plates as described in step 2.1.1.2.

            4. 2.1.4. Cromogen/Substrate

              1. 2.1.4.1. Add 200 μl/well of chromogen/substrate solution (10 ml of 80,6...

              2. 2.1.4.2. Read the plates at 600 nm (or 620 nm).

          2. 2.2. Interpretation of the results

            1. 2.2.1. Calculate the cut-off value by adding 0,6 to the value...

            2. 2.2.2. Test samples giving absorbance values lower than the cut-off are...

            3. 2.2.3. Test samples giving absorbance values greater than the cut-off +...

            4. 2.2.4. Test samples giving intermediate absorbance values are doubtful and a...

        3. 3. BLOCKING ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE...

          1. 3.1. Test procedure

            1. 3.1.1. ELISA plates

              1. 3.1.1.1. Coat ELISA plates with recombinant AHSV-4 VP7 diluted in carbonate/bicarbonate...

              2. 3.1.1.2. Wash the plates five times with phosphate buffered saline (PBS)...

              3. 3.1.1.3. Stabilise the plate by treatment with a stabilising solution (in...

            2. 3.1.2. Test samples and controls

            3. 3.1.3. Conjugate

            4. 3.1.4. Wash the plates five times with PBST and blot dry...

            5. 3.1.5. Chromogen/Substrate

            6. 3.1.6. Reading

          2. 3.2. Interpretation of the results

            1. 3.2.1. Assay validation

            2. 3.2.2. Cut-off calculation

            3. 3.2.3. Interpretation of results

    5. ANNEX V

      1. PART A

      2. PART B

    6. ANNEX VI

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