The Animal By-Products (Wales) Regulations 2003

Regulation 15

SCHEDULE 1 Additional requirements for biogas and composting plants

PART 1

Premises

1.—(1) There must be —

(a) a reception area in which untreated animal by-products (including catering waste) are received,

(b) an area in which vehicles and containers are cleansed and disinfected with adequate facilities for doing this, and

(c) a clean area in which treated compost or digestion residue are stored.

(2) The clean area must be, adequately separated from the reception area and the area in which vehicles and containers are cleansed and disinfected so as to prevent contamination of the treated material. Floors must be laid so that liquid cannot seep into the clean area from the other areas.

(3) The reception area must be easy to clean and disinfect and must have an enclosed and lockable place or container to receive and store the untreated animal by-products.

2.  The animal by-products must be unloaded in the reception area and either —

(a) treated immediately, or

(b) stored in the reception area and treated without undue delay.

3.  The plant must be operated in such a way that —

(a) treated material is not contaminated by untreated or partially treated material or liquids arising from it; and

(b) partially treated material is not contaminted with material which has not been treated to the same extent or liquids arising from it.

4.  The operator shall identify, control and monitor suitable critical points in the operation of the plant to demonstrate that —

(a) these Regulations and the Community Regulation are complied with;

(b) treated material is not contaminated by untreated or partially treated material or liquids arising from it; and

(c) partially treated material is not contaminated with material which has not been treated to the same extent or liquids arising from it.

5.  Containers, receptacles and vehicles used for transporting untreated animal by-products must be cleaned in the dedicated are before they leave the premises and before any treated material is loaded. In the case of vehicles transporting only untreated catering waste and not subsequently transporting treated material, only the wheels of the vehicle need be cleaned.

PART II Treatment systems and parameters for catering waste

1.  Unless an approval specifically permits a different system, catering waste must be treated by one of the systems specified in the table below. The system must ensure that the material is treated to the following parameters:

Composting

System	Composting in a closed reactor	Composting in a closed reactor	Composting in housed windrows
Maximum Particle size	40cm	6cm	40cm
Minimum Temp-erature	60°C	70°C	60°C
Minimum Time spent at the minimum Temp-erature	2 days	1 hour	8 days (during which the windrow must be turned at least 3 times at no less than 2 days intervals)

The time temperature requirements will be achieved as part of the composting process

Biogas

System	Biogas in a closed reactor	Biogas in a closed reactor
Maximum Particle size	5cm	6cm
Minimum Temperature	57°C	70°C
Minimum time spent at the minimum temperature	5 hours	1 hour

2.  The approval must normally specify one of the methods in the table, but the National Assembly may approve a different system if it is satisfied that it achieves the same reduction in pathogens as those methods (including any additional conditions imposed on those methods) in which case the approval must fully describe the whole system.

Composting plants

3.  If the approval for a composting plant specifies one of the methods in the table, it must in addition have as a condition either that —

(a) measures will be taken at source to ensure that meat is not included in the catering waste and that following treatment the material is stored for at least 18 days, or

(b) following the first treatment, the material will be treated again using one of the methods in the table and specified in the approval (not necessarily the same method as was used for the first treatment) except that, if the treatment is in a windrow, the second treatment need not be in a housed windrow.

Biogas plants

4.  The approval of a biogas plant must specify one of the methods in the table and in addition require that either —

(a) measures will be taken at source to ensure that meat is not included in the catering waste; or

(b) following treatment the material is stored for at least 18 days after treatment (storage need not be in an enclosed system).

Regulation 21

SCHEDULE 2 Testing Methods

PART I METHOD FOR THE ISOLATION OF CLOSTRIDIUM PERFRINGENS

Time of testing

1.  Tests must be begun on receipt of the sample or on the first working day which allows this method to be completed. If the test is not begun on the day of receipt the sample must be stored in a refrigerator at between 2°C and 8°C until required. If the sample has been refrigerated it must be removed from the refrigerator and stored at room temperature for at least one hour before the test is started.

Samples

2.  Tests must be carried out using two 10 gram portions of each sample submitted for testing. Each 10 gram sample must be placed aseptically in a sterile container containing 90 ml Clostridium perfringens diluent consisting of 0.1% peptone and 0.8% sodium chloride at a pH of 7 and mixed thoroughly until the sample is evenly suspended.

Inoculations

3.  For each portion of the sample 1 ml of solution must be transferred to a sterile 90 mm petri dish (in duplicate), to which 15 ml of Shahidi - Ferguson agar (SF agar)(1) at a temperature of 47°C±1°C must be added and immediately gently mixed by swirling the dish with 5 clockwise and 5 anticlockwise circular movements.

4.  Once the agar has set, each agar plate must be overlaid with a further 10 ml SF agar at a temperature of 47°C±1°C. Once the overlay has set and with the plate lids uppermost the plates must be incubated anaerobically at 37°C±1°C for 20 hours±2 hours.

Samples with colonies of Clostridium perfringens

5.  After incubation each set of duplicate plates must be examined for colonies characteristic of Clostridium perfringens (black). The sample provisionally fails if any colonies characteristic of Clostridium perfringens are present, in which case the following procedure must be followed to establish whether or not the colonies are Clostridium perfringens.

6.  In the case of each plate, 10 characteristic colonies of Clostridium perfringens must be subcultured on to a further SF agar plate. If there are less than 10 colonies on the plate, all characteristic colonies must be subcultured on to the further plate. The plates must be incubated anaerobically at 37°C±1°C for 20 hours±2 hours.

7.  If the surface area of the plates is overgrown and it is not possible to select well isolated characteristic colonies, 10 suspect colonies must be subcultured on to duplicate SF agar plates and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.

8.  One characteristic colony from each plate must be subcultured on to SF agar and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.

Subcultured colonies

9.  After incubation each plate must be examined for colonies characteristic of Clostridium perfringens. All colonies characteristic of Clostridium perfringens must be —

(a) stab inoculated into motility nitrate medium(2); and

(b) inoculated into either lactose gelatin medium(3) or charcoal gelatin discs(4);

and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.

Examination of subcultures

Motility

10.  The motility nitrate medium must be examined for the type of growth along the stab line. If there is evidence of diffuse growth out into the medium away from the stab line, the bacteria must be considered to be motile.

Reduction of nitrate to nitrite

11.  After examination of the motility nitrate medium, 0.2 ml to 0.5 ml of nitrite detection reagent must be added to it. The formation of a red colour confirms that the bacteria have reduced nitrate to nitrite. Cultures that show a faint reaction (i.e. a pink colour) must be discounted. If no red colour is formed within 15 minutes, a small amount of zinc dust must be added and the plate allowed to stand for 15 minutes. If a red colour is formed after the addition of zinc dust no reduction of nitrate to nitrite has taken place.

Production of gas and acid from lactose and liquefaction of gelatin

12.  The lactose gelatin medium must be examined for the presence of small gas bubbles in the medium.

13.  The lactose gelatin medium must be examined for colour. A yellow colour indicates fermentation of lactose.

14.  The lactose gelatin medium must be chilled for one hour at 2 — 8°C and then checked to see if the gelatin has liquefied. If the medium has solidified it must be re-incubated anaerobically for a further 18 — 24 hours, the medium chilled for a further one hour at 2 — 8°C and again checked to see if the gelatin has liquefied.

15.  The presence of Clostridium perfringens must be determined on the basis of the results from paragraphs 10 to 14. Bacteria which produce black colonies on SF agar, are non-motile, reduce nitrate to nitrite, produce gas and acid from lactose and liquefy gelatin within 48 hours must be considered to be Clostridium perfringens.

Control Tests

16.  Control tests must be carried out each day that a test is initiated using —

(a) Clostridium perfringens no more than seven days old at the time of use;

(b) Escherichia coli NCTC 10418(5) or equivalent not more than seven days old at the time of use; and

(c) processed animal protein or compost or digestion residue which is free of Clostridium perfringens.

17.  10 gram portions of the rendered animal protein must be placed aseptically in each of two sterile containers containing 90 ml Buffered Peprone Water (BPW)(6) and mixed thoroughly until the samples are evenly suspended.

18.  One colony of Clostridium perfringens must be placed in 10 ml BPW and mixed to form an even suspension. 0.1 ml of the suspension must be added to the suspension in the preceding paragraph. This must be repeated for Escherichia coli.

19.  These are then treated and examined in the same way as test samples. If no typical colonies are formed then that day’s testing must be invalid and must be repeated.

PART II METHODS FOR THE ISOLATION OF SALMONELLA

A. BACTERIOLOGICAL METHOD

1.  Tests must be begun on receipt of the sample or on the first working day which allows this method to be completed. If the test is not begun on the day of receipt the sample must be stored in a refrigerator until required. If the sample has been refrigerated it must be removed from the refrigerator and stored at room temperature for at least four hours before the test is started.

Day 1

2.  Tests must be carried out in duplicate using two 25 gram portions of each sample submitted for testing. Each 25 gram sample must be placed aseptically in a sterile container containing 225 ml Buffered Peptone Water (BPW) and incubated at 37°C±1 °C for 18 hours±2 hours.

Day 2

3.  0.1 ml from the jar of incubated BPW must be inoculated into 10 ml Rappaports Vassiliadis broth (RV broth)(7) and incubated at 41.5°C±0.5°C for 24 hours±3 hours.

Day 3

4.  The RV broth must be plated out on to two 90 millimetre plates of Brilliant Green Agar (BGA)(8) or on to one 90 millimetre plate of BGA and one 90 millimetre plate of Xylose Lysine Deoxycholate Agar (XLD)(9) using a 2.5 mm diameter loop. The plates must be inoculated with a droplet taken from the edge of the surface of the fluid by drawing the loop over the whole of one plate in a zig zag pattern and continuing to the second plate without recharging the loop. The space between the loop streaks must be 0.5 cm — 1.0 cm. The plates must be incubated at 37°C ±2°C for 24 ± 3 hours.

5.  The residual RV broth must be reincubated at 41.5°C±0.5°C for a further 24 hours.

Day 4

6.  The plates must be examined and a minimum of 3 colonies from each plate showing suspicion of Salmonella growth must be subcultured —

(a) on to a blood agar plate;

(b) on to a MacConkey agar plate(10); and

(c) into biochemical media suitable for the identification of Salmonella.

These media must be incubated at 37°C overnight.

7.  The reincubated RV broth must be plated out as described in paragraph 4.

Day 5

8.  The incubated composite media or equivalent must be examined and the findings recorded, discarding cultures which are obviously not Salmonella. Slide serological tests must be performed using Salmonella polyvalent “O” and polyvalent “H” (phase 1 and 2) agglutinating sera on selected suspect colonies collected from the blood agar or MacConkey plates. If reactions occur with one or both sera, the colonies must be typed by slide serology and a subculture sent to a Regional Veterinary Laboratory of the Veterinary Laboratories Agency of the Department for Environment, Food and Rural Affairs for further typing.

9.  The plates referred to in paragraph 7 must be examined and further action taken as in paragraph 6 and 8.

B. ELECTRICAL CONDUCTANCE METHOD

1.  Tests must be begun on receipt of the sample or on the first working day which allows the following method to be completed. If the test is not begun on the day of receipt the sample must be stored in a refrigerator until required. If the sample has been refrigerated it must be stored at room temperature for at least four hours before the test is started.

Day 1

2.  Tests must be carried out in duplicate using two 25 gram portions of each sample submitted for testing. Each 25 gram sample must be placed aseptically in a sterile container containing 225 ml Buffered Peptone Water/Lysine/Glucose (BPW/L/G)(11) and incubated at 37°C for 18 hours.

Day 2

3.  The incubated BPW/L/G must be added to Selenite Cystine Trimethylamine-N-Oxide Dulcitol (SC/T/D)(12) and Lysine Decarboxylase Glucose (LD/G)(13) media in electrical conductance cells or wells. For cells or wells containing more than 5 ml medium 0.2 ml of the BPW/L/G must be added and for cells or wells containing 5 ml or less medium 0.1 ml of the BPW/L/G must be added. Cells or wells must be connected to appropriate electrical conductance measuring equipment set to monitor and record changes in electrical conductance at 6 minute intervals over a 24 hour period. The temperature of cells and wells must be kept at 37°C.

Day 3

4.  At the end of the 24 hour period, the information recorded by the conductance measuring equipment must be analysed and interpreted using criteria defined by the manufacturers of the equipment. Where a well or cell is provisionally identified as being positive for Salmonella, the result must be confirmed by subculturing the contents of the well or cell on to two 90 millimetre plates of BGA or on to one 90 millimetre plate of BGA and one 90 millimetre plate of Xylose Lysine Deoxycholate Agar (XLD) using a 2.5 mm diameter loop. The plates must be inoculated with a droplet taken from the edge of the surface of the fluid by drawing the loop over the whole of one plate in a zig zag pattern and continuing to the second plate without recharging the loop. The space between the loop streaks must be 0.5 cm — 1.0 cm. The plates must be incubated at 37°C overnight.

Day 4

5.  The plates must be examined and a minimum of 3 colonies from each plate showing suspicion of Salmonella growth must be subcultured —

(a) on to a blood agar plate;

(b) on to a MacConkey agar plate; and

(c) into biochemical media suitable for the identification of Salmonella.

These media must be incubated at 37°C overnight.

Day 5

6.  The incubated composite media or equivalent must be examined and the findings recorded, discarding cultures which are obviously not Salmonella. Slide serological tests must be performed using Salmonella polyvalent “O” and polyvalent “H” (phase 1 and 2) agglutinating sera on selected suspect colonies collected from the blood agar or MacConkey plates. If reactions occur with one or both sera, a subculture must be sent to a Regional Veterinary Laboratory of the Veterinary Laboratories Agency of the Department for Environment, Food and Rural Affairs for further typing.

PART III METHOD FOR THE ISOLATION OF ENTEROBACTERIACEAE

1.  Tests must be begun on receipt of the sample or on the first working day which allows this method to be completed. If the test is not begun on the day of receipt the sample must be stored in a refrigerator until required at between 2°C and 8°C. If the sample has been refrigerated it must be removed from the refrigerator and stored at room temperature for at least one hour before the test is started.

Samples

2.  Tests must be carried out using five 10 gram portions of each sample submitted for testing. Each 10 gram sample must be placed aseptically in a sterile container containing 90 ml Buffered Peptone Water and mixed thoroughly until the sample is evenly suspended.

Inoculations

3.  For each portion of the sample 1 ml of solution must be transferred to a sterile 90 mm petri dish (in duplicate). The plates must be labelled to identify the portion of sample they were taken from. 15 ml of Violet Red Bile Glucose Agar (VRBGA)(14) at a temperature of 47°C±2°C must be added to each petri dish and immediately gently mixed by swirling the dish with five clockwise and five anticlockwise circular movements.

4.  Once the agar has set, each agar plate must be overlaid with a further 10 ml VRBGA at a temperature of 47°C±2°C. Once the overlay has set, the plates must be inverted and incubated aerobically at 37°C±1°C for 20 hours±2 hours.

Samples with colonies of Enterobacteriaceae

5.  After incubation each set of duplicate plates must be examined for colonies characteristic of Enterobacteriaceae (purple colonies 1 — 2 mm in diameter). All characteristic colonies on each plate must be counted and the arithmetic mean of the duplicate plates taken.

The sample provisionally fails if either—

(a) any arithmetic mean is above 30(15); or

(b) three or more arithmetic means are above 10;

in which case the following procedure must be followed to establish whether or not the colonies are Enterobacteriaceae.

6.  After counting the colonies, characteristic colonies must be taken at random from the agar plates, the number being at least the square root of the colonies counted. The colonies must be subcultured onto a blood agar plate and incubated aerobically at 37°C±1°C for 20 hours±2 hours.

Examination of subcultures

7.  An oxidase test and a glucose fermentation test must be performed on each of the five subcultured colonies. Colonies which are oxidase-negative and glucose fermentation-positive must be considered to be Enterobacteriaceae.

8.  If not all of the colonies prove to be Enterobacteriaceae, the total count in paragraph 5 must be reduced in proportion prior to establishing whether or not the sample should fail.

Controls

9.  Control tests must be carried out each day that a test is initiated using —

(a) Escherichia coli NCTC 10418 no more than seven days old at time of use; and

(b) processed animal protein or compost or digestive residue which is free of Enterobacteriaceae.

10.  A 10 gram portion of the rendered animal protein must be placed aseptically in a sterile container containing 90 ml BPW and mixed thoroughly until the sample is evenly suspended.

11.  One colony of Escherichia coli must be placed in 10 ml BPW and mixed to form an even suspension. 0.1 ml of the suspension must be added to the suspension in the preceding paragraph.

12.  This is then treated and examined in the same way as test samples. If no typical colonies are formed then that day’s testing must be invalid and must be repeated.

regulation 50

SCHEDULE 3 Transitional Measures

PART I INTRA-SPECIES RECYCLING BAN FOR FISH(16)

1.  In accordance with Article 1 of Commission Regulation (EC) No. 811/2003 implementing Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the intra-species recycling ban for fish, the burial and burning of animal by-products and certain transitional measures, the prohibition on the feeding of fish with processed animal protein derived from the bodies or parts of bodies of fish of the same species in Article 22(1)(a) of the Community Regulation shall not apply.

PART II COLLECTION, TRANSPORTATION AND DISPOSAL OF FORMER FOODSTUFFS(17)

1.—(1) The National Assembly will be the competent authority for granting approvals under Commission Regulation (EC) No. 813/2003 on transitional measures under Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the collection, transport and disposal of former foodstuffs.

(2) Instructions, for the purposes of Article 3(3) of that Regulation, of the competent authority may be issued by an inspector.

2.  For the purposes of Article 1(1) of Commission Regulation (EC) No. 813/2003, by way of derogation from Article 6(2)(f) and Article 7 of the Community Regulation, former foodstuffs which have not been mixed with any other animal by-products (other than Category 3 catering waste) may be collected, transported and disposed of or treated in the same way as catering waste.

3.  Where former foodstuffs are mixed with Category 1 or Category 2 material any person in possession or control of the material must ensure that it is disposed of in accordance with Article 1(2) of Commission Regulation (EC) No. 813/2003; and any person who fails to do so will be guilty of an offence.

4.  Where former foodstuffs are sent for disposal in an approved landfill site, any person in possession or control of the material must comply with Article 1(3) of Commission Regulation (EC) No. 813/2003 and any person who fails to do so will be guilty of an offence.

5.  Any person who fails to comply with any instructions given by an inspector under Article 3(3) of Commission Regulation (EC) No. 813/2003 will be guilty of an offence.

6.  In this Part “former foodstuffs” does not include waste from the production of products which are intended to be cooked before they are eaten.

PART III USKED COOKING OIL IN ANIMAL FEED(18)

Scope

1.  Notwithstanding the prohibition on feeding farmed animals with catering waste or feed materials containing or derived from catering waste used cooking oil may be used for the production of animal feed if it has been collected, treated and blended in accordance with this Part.

2.  This Part is confined to used cooking oil which —

(a) originates exclusively in restaurants, catering facilities and kitchens, including central kitchens and household kitchens; and

(b) is intended for the production of animal feed.

Approvals

3.—(1) The National Assembly may approve —

(a) collectors of used cooking oil if it is satisfied that the collector will comply with the requirements of this Part; and

(b) operators of premises on which used cooking oil is treated or mixed with other oils if it is satisfied that the premises and operation comply with the requirements of this Part.

(2) The approval shall only be granted if the collector or operator was collecting, treating or blending used cooking oils on 1st November 2002.

4.  The approval must specify —

(a) the name of the operator and the address of the approved premises;

(b) in the case of treatment premises, the parts of the premises in which used cooking oil may be received and treated; and

(c) the expiry date, which shall be no later than 31 October 2004.

5.—(1) Approval must be suspended immediately if the conditions under which it was granted are no longer fulfilled.

(2) Once suspended, the approval may only be reinstated subject to fulfilment of the requirements of the Community Regulation in their entirety.

General obligations

6.—(1) Used cooking oil must be collected, transported, stored, handled, treated, and used in accordance with this Part.

(2) Any person who fails to comply with paragraph (1) will be guilty of an offence.

(3) Any used cooking oil which does not comply with the provisions of this Part must be disposed of as directed by notice by an inspector.

7.  Used cooking oil must be —

(a) collected by an approved collector;

(b) treated by an approved operator on approved treatment premises; and

(c) mixed with other oils by an approved operator on approved blending premises.

Collection and transportation of used cooking oil

8.—(1) Used cooking oil must be collected and transported in lidded containers or leak proof vehicles and identified in such a way that the contents, even after mixing, are traceable to all the premises of origin.

(2) Collectors must take all necessary measures to ensure that the used cooking oil collected is free from contamination by harmful substances.

(3) Reusable containers, and all reusable items of equipment or appliances that come into contact with used cooking oil, must be cleaned, washed and disinfected after each use.

(4) Vehicles or containers which carry any material which could contaminate the used cooking oil must be thoroughly cleansed and disinfected before they are used to carry used cooking oil.

Approved premises and the operation of blending premises

9.  The operator of an approved premises must ensure that the premises comply with, and are operated in accordance with, the provisions of this Part.

10.—(1) Before mixing with other oil operators of blending premises must ensure that each batch of used cooking oil is tested to ensure compliance with the standards in paragraph 16 of this Part. A batch must be no greater than 30 tonnes.

(2) Collectors and operators of approved premises must ensure that used cooking oil that does not comply with the standards in paragraph 16 of this Part is not used for animal feed.

Approved premises

11.—(1) Approved premises must be constructed in such a way that they are easy to clean and disinfect.

(2) Unauthorised persons and animals must not have access to the premises.

(3) The premises must have adequate facilities for cleaning and disinfecting the containers or receptacles in which used cooking oil is received and, where appropriate, the vehicles in which it is transported.

(4) The premises must have adequate lavatories and washing facilities for staff.

(5) The premises must have a covered space, clearly marked, to receive used cooking oil.

(6) Where appropriate, the premises must have a separate storage area for any used cooking oil that is not suitable for use in animal feed.

(7) Tanks must be sealed with vents located and screened in a manner that prevents entry by contaminants or pests.

(8) Pipework must be sealed when not in use.

Operators'own-checks

12.—(1) Operators of approved premises must adopt all measures necessary to comply with the requirements of this Part.

(2) They must put in place, implement and maintain a procedure developed in accordance with the principles of the system of hazard analysis and critical control points (HACCP).

(3) They must in particular —

(a) identify and control the critical control points in the premises;

(b) establish and implement methods for monitoring and checking such critical control points and keep records of such checks for at least two years; and

(c) ensure the traceability of each batch received and despatched.

13.—(1) The operator of approved blending premises must carry out checks and take samples for the purposes of checking compliance with the standards in paragraph 16.

(2) Where the results of a check or a test show that the used cooking oil does not comply with the provisions of this Part, the operator must —

(a) establish the causes of failures of compliance;

(b) ensure that no oil is despatched for use in feedingstuffs;

(c) instigate appropriate decontamination and cleaning procedures; and

(d) where used cooking oil has already been despatched for use in feedingstuffs, or incorporated into feedingstuffs, take all necessary measures to ensure that feedingstuffs containing the oil are not fed to livestock.

14.—(1) The operator must record the results of the checks and tests.

(2) The operator must keep a sample of each consignment of used cooking oil despatched from the premises for at least six months from the date of despatch.

Hygiene requirements in approved premises

15.—(1) Containers, receptacles and, where appropriate, vehicles used for transporting used cooking oil must be cleaned in a designated area.

(2) Preventive measures against birds, rodents, insects or other vermin must be taken systematically.

(3) Used cooking oil intended for use in animal feed must not be stored in the same area as used cooking oil which is not suitable for use in animal feed or products which may pose a risk to animal or human health.

(4) Cleaning procedures must be established and documented for all parts of the premises.

(5) Hygiene control must include regular inspections of the environment and equipment.

(6) Inspection schedules and results must be recorded.

(7) Installations and equipment must be kept in a good state of repair.

(8) Measuring equipment must be calibrated at least once a year.

(9) Tanks and pipes must be cleaned internally at least once a year or when there is build-up of water and physical contaminants.

(10) Treated used cooking oil must be handled and stored in such a way as to preclude contamination.

Specification for used cooking oil for use in animal feed

16.—(1) Used cooking oil must meet the following minimum standards before use in animal feed.

(2) Physical contamination:

(a) moisture and impurities: <3%

(b) impurities: <0.15%.

(3) Presence of mineral oil: absence.

(4) Presence of oxidised fatty acids: >88% Elutable Fatty acid content.

(5) Presence of pesticide residues complies with Directive 2002/32/EC(19) of the European Parliament and the Council on undesirable substances in animal feed.

(6) Presence of PCBs: <100ppb for the 7 main congeners(20).

(7) Presence of Salmonella: absence.

(8) Presence of animal fat:

(a) Pentadecanoic acid (C15): <0.2%

(b) Cis.9 – hexadecanoic acid (C16:1): <2%

(c) Heptadeconic acid (C17): <0.4%

(d) Cis.9 – heptadecanoic acid (C17:1) <0.3%

(e) Fatty acides with a chain length of 20 carbon atoms or more (C20+): <5%

Commercial documents

17.—(1) Commercial documents may be in written or electronic form.

(2) A written commercial document or a printout of an electronic document must accompany the consignment of used cooking oil during transportation.

(3) The producer, receiver and carrier must each retain a copy of a written commercial document or, for electronic information, a record of that information.

(4) Commercial documents must contain the following information —

(a) the address of the premises from which the used cooking oil was taken;

(b) the date on which the used cooking oil was taken from the premises;

(c) the quality and description of the used cooking oil;

(d) the quantity of the used cooking oil;

(e) the name and the address of the carrier;

(f) the destination of the used cooking oil; and

(g) a unique reference number that links the collector and the container or vehicle to the premises from which the used cooking oil was taken.

Records

18.—(1) Any person consigning, transporting or receiving used cooking oil must keep a record containing the information specified in the commercial document.

(2) For used cooking oil which is sutable for use in animal feed, the records must in addition provide for full traceability of the oil from the premises of origin to its incorporation into animal feed.

(3) For used cooking oil which is not suitable for use in animal feed, the person consigning the oil for disposal must in addition keep a record showing the method and place of disposal and the date the oil was consigned for disposal.

List of premises

19.—(1) The National Assembly shall maintain a list of the names and addresses of approved:

(a) collectors of used cooking oil;

(b) operators of treatment premises; and

(c) operators of blending premises.

(2) Each collector and operator of approved premises must be assigned an official identification number.

(3) The National Assembly will make this list publicly available.

PART IV MAMMALIAN BLOOD(21)

General

1.  By way of derogation from Annex VII, Chapter 11, paragraph 1 of the Community Regulation, mammalian blood may be processed in accordance with this Part.

2.  The National Assembly may approve the use of processing methods 2 to 5 or 7 of Annex V of the Community Regulation for the processing of mammalian blood.

3.—(1) Approval must be suspended immediately if the conditions under which it was granted are not fulfilled.

(2) Once suspended, the approval may only be reinstated subject to fulfilment of the requirements of the Community Regulation in their entirety.

(3) Any material not processed in accordance with this Part or the Community Regulation must be disposed of as instructed by an inspector.

4.  The approval may only be granted if the operator was processing at these premises, using that equipment and using those methods on 1st November 2002.

5.  All other relevant provisions of the Community Regulation must be complied with.

PART V OLEOCHEMICAL PLANTS USING RENDERED FATS FROM CATEGORY 2 AND CATEGORY 3 MATERIALS(22)

General obligations

1.  By way of derogation from article 14 of the Community Regulation, the National Assembly may approve the use of oleochemical plants to process rendered fats derived from both Category 2 and Category 3 material providing they comply with the following conditions.

2.—(1) Approval must be suspended immediately if the conditions under which it was granted are not fulfilled.

(2) Once suspended, the approval may only be reinstated subject to fulfilment of the requirements of the Community Regulation in their entirety.

(3) Any material not processed in accordance with this Part or the Community Regulation must be disposed of as instructed by an inspector.

3.  The approval may only be granted to premises and facilities that operated in that way on 1st November 2002.

Specific requirements

4.—(1) Only rendered fats derived from Category 2 and Category 3 materials may be used.

(2) Rendered fats derived from Category 2 materials must be processed in accordance with the standards in Chapter III of Annex VI of the Community Regulation.

(3) Additional processes such as distillation, filtration and processing with absorbents must be used to further improve the safety of the tallow derivatives.

PART VI LOW CAPACITY INCINERATION OR CO-INCINERATION PLANTS WHICH DO NOT INCINERATE OR CO-INCINERATE SPECIFIED RISK MATERIALS OR CARCASES CONTAINING THEM(23)

General obligations

1.  By way of derogation from Article 12(3) of the Community Regulation, the National Assembly may approve the use of low capacity incineration or co-incineration plants which do not meet the requirements laid down in Annex IV to the Community Regulation if they are operated in accordance with this Part.

2.—(1) Approval must be suspended immediately if the conditions under which it was granted are not fulfilled.

(2) Once suspended, the approval may only be reinstated subject to fulfilment of the requirements of the Community Regulation in their entirety, including Annex IV.

(3) Any material not incinerated in accordance with this Part or the Community Regulation must be disposed of as instructed by an inspector.

3.  The approval may only be granted to incinerators that were in operation on 1st November 2002.

Specific requirements

4.  The operator shall take all necessary measures to ensure that —

(a) animal by-products are handled and stored safely and incinerated or co-incinerated without undue delay in such a way that they are reduced to dry ash;

(b) the dry ash is disposed of properly and records are kept of the quantity and description of the animal by-products incinerated and the date of incineration;

(c) the dry ash is not removed from the combustion chamber unless combustion is complete; and

(d) transport and intermediate storage of the dry ash takes place in a closed container to prevent dispersal in the environment and is disposed of safely;

and failure to do so will be an offence.

5.  In the case of a breakdown or malfunction, the operator must reduce or close down operations as soon as practicable until normal operations can be resumed and failure to do so will be an offence.

Regulation 51

SCHEDULE 4

Amendments

Amendments to the TSE (Wales) Regulations 2002

1.  The TSE (Wales) Regulations 2002(24) are amended in accordance with this Schedule.

2.  Regulations 33(4), 34(2),52, 54, 56(1)(a), 56(2)(b), 56(4)(c) and (d), 63 to 68, 69(1), (3), (4) and (5) and Schedule 6 are revoked.

3.  At the end of the regulation 13 there shall be added —

“(7) In this Regulation mammalian meat and bone meal does not include any compost or digestion residues resulting from the treatment of animal by-products in a composting or biogas plant in accordance with the Animal By-Products Regulations 2003.”.

4.  After regulation 34 there shall be inserted —

“Mixing specified risk material with other animal material

34A.   Any animal material that comes into contact with, or is mixed with, specified risk material must be treated as specified risk material.”.

5.  For regulation 40 there is substituted the following regulation—

“40.   Once specified risk material has been removed from the carcase and treated in accordance with this Part of these Regulations, including any material treated as if it were specified risk material in accordance with regulation 33(5) or 34(4) above, or, in the case of specified solid waste, recovered from the drainage system, the person responsible for its removal or recovery must, without unreasonable delay, send it directly to —

(a) be handled in accordance with the Animal By-Products Regulations 2003; or

(b) to premises licenced under regulation 57.”

6.  For Schedule 5 (Application of Part IV of the Regulations to scheme animals) there shall be substituted the following Schedule —

SCHEDULE 5 APPLICATION OF PART IV OF THE REGULATIONS TO SCHEME ANIMALS

PROVISION OF THE REGULATIONS	EXTENT TO WHICH THE PROVISON APPLIES TO SCHEME ANIMALS
Regulation 33(3)	Not applicable
Regulation 33(4)	Subject to the modification that from the point at which specified risk material derived from a scheme animal is removed from the slaughterhouse, it may come into contact with any other animal material from such an animal
Regulation 34	Not applicable
Regulation 39(3)(b)	Not applicable
Regulation 57	Not applicable

(1)

Shahidi-Ferguson agar- See Shahidi, S. A. and Ferguson, A. R. (1971) Applied Microbiology 21:500-506.American Society for Microbiology, 1913 1 St N.W., Washington DC 20006, USA.

(2)

Motility nitrate medium-See Hauschild AHW, Gilbert RJ, Harmon SM, O'Keefe MF, Vahlefeld R, (1997) ICMSF Methods Study VIII, Canadian Journal of Microbiology 23, 884-892. National Research Council of Canada, Ottawa ON K1A oR6, Canada.

(3)

Lactose gelatin medium- See Hauschild AHW, Gilbert RJ, Harmon SM, O'Keefe MF, Vahlefield R, (1997) ICMSF Methods Study VIII, Canadian Journal of Microbiology 23, 884-892.

(4)

Carcoal gelatin discs- See Mackie and McCartney, (1996) Practical Medical Microbiology 14, 509. Churchill Livingstone, Robert Stevenson House, 1-3 Baxter’s Place, Leith Walk, Edinburgh EH1 3AF.

(5)

The National Collection of Type Cultures, Central Public Health Laboratory, 61 Colindale Ave, London NW9 5HT.

(6)

Buffered Peptone Water — See Edel, W. and Kampelmacher, E.H. (1973) Bulletin of World Health Organisation, 48: 167-174, World Health Organisation Distribution and Sales, CH-1211, Geneva 27, Switzerland (ISSN 0042-9686).

(7)

Rappaports Vassiliadis Broth — See Vassiliadis P, Pateraki E, Papaiconomou N, Papadkis J A, and Trichopoulos D (1976) Annales de Microbiologie (Institute Pasteur) 127B: 195-200, Elsevier, 23 rue Linois, 75724 Paris, Cedex 15, France.

(8)

Brilliant Green Agar — See Edel W and Kampelmacher E H (1969) Bulletin of World Health Organisation 41:297-306, World Health Organisation Distribution and Sales, CH-1211, Geneva 27, Switzerland (ISSN 0042-9686).

(9)

Xylose Lisene Deoxycholate Agar — See Taylor W I, (1965) American Journal of Clinical Pathology, 44:471-475, Lippincott and Raven, 227E Washington Street, Philadelphia PA 19106, USA.

(10)

MacConkey agar — See (1963) International Standards for Drinking Water, World Health Distribution and Sales, CH-1211, Geneva 27, Switzerland.

(11)

Buffered Peptone Water/Lysine/Glucose — See Ogden I D (1988) International Journal of Food Microbiology 7:287-297, Elsevier Science BV, PO Box 211, 1000 AE, Amsterdam, Netherlands (ISSN 0168-1695).

(12)

Selenite Cystine Trimethylamine-N-Oxide Dulcitol — See Easter, M C and Gibson, D M, (1985) Journal of Hygiene 94:245-262, Cambridge University Press, Cambridge.

(13)

Lysine Decarboxylase Glucose — See Ogden I D (1988) International Journal of Food Microbilogy 7:287-297, Elsevier Science BV, PO Box 211, 1000 AE, Amsterdam, Netherlands (ISSN 0168-1695).

(14)

Violet Red Bile Glucose Agar — See Mossell D A A, Eelderink I, Koopmans M, van Rossem F (1978) Laboratory practice 27 No. 12 1049-1050; Emap Maclaren, PO Box 109, Maclaren House, 19 Scarbrook Road, Croydon CR9 1QH.

(15)

An arithmetic mean of 30 is equivalent to 3x10² colony forming units per gram of original sample.

(16)

This Part of the Schedule enforces Article 1 of Commission Regulation (EC) No. 811/2003 implementing Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the intra-species recycling ban for fish, the burial and burning of animal by-products and certain transitional measures, OJ No. L117, 13.5.2003, p.14.

(17)

This Part of the Schedule enforces Commission Regulation (EC) No. 813/2003 on transitional measures under Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the collection, transport and disposal of former foodstuffs, OJ No. L117, 13.5.2003, p.22.

(18)

This Part of the Schedule enforces Commission Decision 2003/320/EC on transitional measures under Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the use in feed of used cooking oils, OJ No. L117, 13.5.2003, p.24.

(19)

OJ No. L 140, 30.05.2002, p.10.

(20)

ICES7 polychlorinated biphenyls.

(21)

This Part of the Schedule implements Commission Decision 2003/321/EC on transitional measures under Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the processing standards mammalian blood, OJ No. L117, 13.5.2003, p.30.

(22)

This Part of the Schedule implements Commission Decision 2003/326/EC on transitional measures under Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the separation of Category 2 and Category 3 oleochemical plants, OJ No. L117, 13.5.2003, p.42.

(23)

This Part of the Schedule implements Commission Decision 2003/326/EC on transitional measures under Regulation (EC) No. 1774/2002 of the European Parliament and of the Council as regards the low capacity incineration on co-incineration plants which do not incinerate or co-incinerate specified risk material or carcases containing them, OJ No. L117, 13.5.2003, p.44.

(24)

S.I. 2002/1416 (W.142).