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Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC)
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- Directives originating from the EU
- 1964 No. 432
- ANNEX C
- Division 2.
- Division 2.3.
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Changes over time for: Division 2.3.
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[F1 [F2 [F32.3. Complement fixation test (CFT) U.K.
2.3.1. The antigen represents a bacterial suspension in phenol-saline (NaCl 0,85 % (m/v) and phenol at 0,5 % (v/v)) or in a veronal buffer. Antigens may be delivered in the concentrated state provided the dilution factor to be used is indicated on the bottle label. The antigen must be stored at 4 °C and not frozen. U.K.
2.3.2. Serums must be inactivated as follows: U.K.
bovine serum: 56 to 60 °C for 30 to 50 minutes,
porcine serum: 60 °C for 30 to 50 minutes.
2.3.3. In order to carry out the genuine reaction within the test procedure, a complement dose higher than the minimum necessary for total haemolysis shall be used. U.K.
2.3.4. In carrying out the complement fixation test, the following controls must be made each time: U.K.
(a)
control of the anti-complementary effect of the serum;
(b)
control of the antigen;
(c)
control of sensitised red blood cells;
(d)
control of the complement;
(e)
control using a positive serum of sensitivity at the start of the reaction;
(f)
control of the specificity of the reaction using a negative serum.
2.3.5. Calculation of results U.K.
The OIEISS contains 1 000 international CFT units (ICFTU) per ml. If the OIEISS is tested in a given method the result is given as a titre (i.e. highest direct dilution of the OIEISS giving 50 % haemolysis, T OIEISS ). The test result for the test serum given as titre (T TESTSERUM ) must be expressed in ICFTU per ml. In order to convert the expression of a titre into ICFTU, the factor (F) necessary to convert a titre of an unknown test serum (T TESTSERUM ) tested by that method into the ICFTU expression can be found from the formula:
F = 1 000 × 1/T OIEISS
and the content of international CFT units per ml of test serum (ICFTU TESTSERUM ) from the formula:
ICFTU TESTSERUM = F × T TESTSERUM
2.3.6. Interpretation of results U.K.
A serum containing 20 or more ICFTU per ml shall be considered to be positive.] ] ]
Textual Amendments
F1 Substituted by Council Directive 97/12/EC of 17 March 1997 amending and updating Directive 64/432/EEC on health problems affecting intra-Community trade in bovine animals and swine.
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