Commission Directive 2008/128/EC (repealed)Show full title

ANNEX I U.K.

A. GENERAL SPECIFICATIONS FOR ALUMINIUM LAKES OF COLOURS U.K.

Definition	Aluminium lakes are prepared by reacting colours complying with the purity criteria set out in the appropriate specification monograph with alumina under aqueous conditions. The alumina is usually freshly prepared undried material made by reacting aluminium sulfate or chloride with sodium or calcium carbonate or bicarbonate or ammonia. Following lake formation, the product is filtered, washed with water and dried. Unreacted alumina may also be present in the finished product.
HCl insoluble matter	Not more than 0,5 %
Ether extractable matter	Not more than 0,2 % (under neutral conditions) Specific purity criteria for the corresponding colours are applicable.

B. SPECIFIC CRITERIA OF PURITY U.K.

a Colour intensity is defined as the absorbance of a 0,1 % (w/v) solution of caramel colour solids in water in a 1 cm cell at 610 nm.
b Expressed on equivalent colour basis i.e. is expressed in terms of a product having a colour intensity of 0,1 absorbance units.
c Absorbance ratio of alcohol precipitate is defined as the absorbance of the precipitate at 280 nm divided by the absorbance at 560 nm (1 cm cell).
d Benzene not more than 0,05 % v/v.
E 100 CURCUMIN
Synonyms	CI Natural Yellow 3, Turmeric Yellow, Diferoyl Methane
Definition	Curcumin is obtained by solvent extraction of turmeric i.e. the ground rhizomes of natural strains of Curcuma longa L. In order to obtain a concentrated curcumin powder, the extract is purified by crystallisation. The product consists essentially of curcumins; i.e. the colouring principle (1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,6-dien-3,5-dione) and its two desmethoxy derivatives in varying proportions. Minor amounts of oils and resins naturally occuring in turmeric may be present. Only the following solvents may be used in the extraction: ethylacetate, acetone, carbon dioxide, dichloromethane, n-butanol, methanol, ethanol, hexane.
Class	Dicinnamoylmethane
Colour Index No	75300
Einecs	207-280-5
Chemical names	I 1,7-Bis(4-hydroxy-3-methoxyphenyl)hepta-1,6-diene-3,5-dione II 1-(4-Hydroxyphenyl)-7-(4-hydroxy-3-methoxy-phenyl-)hepta-1,6-diene-3,5-dione III 1,7-Bis(4-hydroxyphenyl)hepta-1,6-diene-3,5-dione
Chemical formula	I C21H20O6 II C20H18O5 III C19H16O4
Molecular weight	I. 368,39 II. 338,39 III. 308,39
Assay	Content not less than 90 % total colouring matters E1 cm 1 %1 607 at ca 426 nm in ethanol
Description	Orange-yellow crystalline powder
Identification
A. Spectrometry	Maximum in ethanol at ca 426 nm
B. Melting Range	179 °C-182 °C
Purity
Solvent residues	Ethylacetate Acetone n-butanol Methanol Ethanol Hexane	Not more than 50 mg/kg, singly or in combination
	Dichloromethane: not more than 10 mg/kg
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 101 (i) RIBOFLAVIN
Synonyms	Lactoflavin
Class	Isoalloxazine
Einecs	201-507-1
Chemical names	7,8-Dimethyl-10-(D-ribo-2,3,4,5-tetrahydroxypentyl)benzo(g)pteridine-2,4(3H,10H)-dione 7,8-dimethyl-10-(1′-D-ribityl)isoalloxazine
Chemical formula	C17H20N4O6
Molecular weight	376,37
Assay	Content not less than 98 % on the anhydrous basis E1 cm 1 % 328 at ca 444 nm in aqueous solution
Description	Yellow to orange-yellow crystalline powder, with slight odour
Identification
A. Spectrometry	The ratio A375/A267 is between 0,31 and 0,33 The ratio A444/A267 is between 0,36 and 0,39	in aqueous solution
	Maximum in water at ca 444 nm
B. Specific rotation	[α]D20 between – 115° and – 140° in a 0,05 N sodium hydroxide solution
Purity
Loss on drying	Not more than 1,5 % after drying at 105 °C for 4 hrs
Sulfated ash	Not more than 0,1 %
Primary aromatic amines	Not more than 100 mg/kg (calculated as aniline)
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 101 (ii) RIBOFLAVIN-5′-PHOSPHATE
Synonyms	Riboflavin-5′-phosphate sodium
Definition	These specifications apply to riboflavin 5′-phosphate together with minor amounts of free riboflavin and riboflavin diphosphate
Class	Isoalloxazine
Einecs	204-988-6
Chemical names	Monosodium (2R,3R,4S)-5-(3′)10′-dihydro-7′,8′-dimethyl-2′,4′-dioxo-10′-benzo[γ]pteridinyl)-2,3,4-trihydroxypentyl phosphate; monosodium salt of 5′-monophosphate ester of riboflavin
Chemical formula	For the dihydrate form : C17H20N4NaO9P·2H2O For the anhydrous form : C17H20N4NaO9P
Molecular weight	541,36
Assay	Content not less than 95 % total colouring matters calculated as C17H20N4NaO9P·2H2O E1 cm 1 % 250 at ca 375 nm in aqueous solution
Description	Yellow to orange crystalline hygroscopic powder, with slight odour and a bitter taste
Identification
A. Spectrometry	The ratio A375/A267 is between 0,30 and 0,34 The ratio A444/A267 is between 0,35 and 0,40	in aqueous solution
	Maximum in water at ca 444 nm
B. Specific rotation	[α]D20 between + 38° and + 42° in a 5 molar HCl solution
Purity
Loss on drying	Not more than 8 % (100 °C, 5 hrs in vacuum over P2O5) for the dihydrate form
Sulfated ash	Not more than 25 %
Inorganic phosphate	Not more than 1,0 % (calculated as PO4 on the anhydrous basis)
Subsidiary colouring matters	Riboflavin (free) : Not more than 6 % Riboflavine diphosphate : Not more than 6 %
Primary aromatic amines	Not more than 70 mg/kg (calculated as aniline)
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 102 TARTRAZINE
Synonyms	CI Food Yellow 4
Definition	Tartrazine consists essentially of trisodium 5-hydroxy-1-(4-sulfonatophenyl)-4-(4-sulfonatophenylazo)-H-pyrazole-3-carboxylate and subsidiary colouring matters together with sodium chloride and/or sodium sulfate as the principal uncoloured components. Tartrazine is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Monoazo
Colour Index No	19140
Einecs	217-699-5
Chemical names	Trisodium-5-hydroxy-1-(4-sulfonatophenyl)-4-(4-sulfonatophenylazo)-H-pyrazole-3-carboxylate
Chemical formula	C16H9N4Na3O9S2
Molecular weight	534,37
Assay	Content not less than 85 % total colouring matters calculated as the sodium salt E1 cm 1 % 530 at ca 426 nm in aqueous solution
Description	Light orange powder or granules
Identification
A. Spectrometry	Maximum in water at ca 426 nm
B. Yellow solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 1,0 %
Organic compounds other than colouring matters:
4-hydrazinobenzene sulfonic acid 4-aminobenzene-1-sulfonic acid 5-oxo-1-(4-sulfophenyl)-2-pyrazoline-3-carboxylic acid 4,4′-diazoaminodi (benzene sulfonic acid) Tetrahydroxysuccinic acid	Total not more than 0,5 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	Not more than 0,2 % under neutral conditions
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 104 QUINOLINE YELLOW
Synonyms	CI Food Yellow 13
Definition	Quinoline Yellow is prepared by sulfonating 2-(2-quinolyl) indan-1,3-dione. Quinoline Yellow consists essentially of sodium salts of a mixture of disulfonates (principally), monosulfonates and trisulfonates of the above compound and subsidiary colouring matters together with sodium chloride and/or sodium sulfate as the principal uncoloured components. Quinoline Yellow is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Chinophthalone
Colour Index No	47005
Einecs	305-897-5
Chemical name	The disodium salts of the disulfonates of 2-(2-quinolyl) indan-1,3-dione (principal component)
Chemical formula	C18H9N Na2O8S2 (principal component)
Molecular weight	477,38 (principal component)
Assay	Content not less than 70 % total colouring matters calculated as the sodium salt Quinoline Yellow shall have the following composition: Of the total colouring matters present: not less than 80 % shall be disodium 2-(2-quinolyl) indan-1,3-dione-disulfonates not more than 15 % shall be sodium 2-(2-quinolyl) indan-1,3-dione-monosulfonates not more than 7,0 % shall be trisodium 2-(2-quinolyl) indan-1,3-dione-trisulfonate E1 cm 1 % 865 (principal component) at ca 411 nm in aqueous acetic acid solution
Description	Yellow powder or granules
Identification
A. Spectrometry	Maximum in aqueous acetic acid solution of pH 5 at ca 411 nm
B. Yellow solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 4,0 %
Organic compounds other than colouring matters:
2-methylquinoline 2-methylquinoline-sulfonic acid Phthalic acid 2,6-dimethyl quinoline 2,6-dimethyl quinoline sulfonic acid	Total not more than 0,5 %
2-(2-quinolyl)indan-1,3-dione	Not more than 4 mg/kg
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	Not more than 0,2 % under neutral conditions
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 110 SUNSET YELLOW FCF
Synonyms	CI Food Yellow 3, Orange Yellow S
Definition	Sunset Yellow FCF consists essentially of disodium 2-hydroxy-1-(4-sulfonatophenylazo) naphthalene-6-sulfonate and subsidiary colouring matters together with sodium chloride and/or sodium sulfate as the principal uncoloured components. Sunset Yellow FCF is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Monoazo
Colour Index No	15985
Einecs	220-491-7
Chemical names	Disodium 2-hydroxy-1-(4-sulfonatophenylazo)naphthalene-6-sulfonate
Chemical formula	C16H10N2Na2O7S2
Molecular weight	452,37
Assay	Content not less than 85 % total colouring matters calculated as the sodium salt E1 cm 1 % 555 at ca 485 nm in aqueous solution at pH 7
Description	Orange-red powder or granules
Identification
A. Spectrometry	Maximum in water at ca 485 nm at pH 7
B. Orange solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 5,0 %
1-(Phenylazo)-2-naphthalenol (Sudan I)	Not more than 0,5 mg/kg
Organic compounds other than colouring matters:
4-aminobenzene-1-sulfonic acid 3-hydroxynaphthalene-2,7-disulfonic acid 6-hydroxynaphthalene-2-sulfonic acid 7-hydroxynaphthalene-1,3-disulfonic acid 4,4′-diazoaminodi(benzene sulfonic acid) 6,6′-oxydi(naphthalene-2-sulfonic acid)	Total not more than 0,5 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	Not more than 0,2 % under neutral conditions
Arsenic	Not more than 3 mg/kg
Lead	Not more than 2 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
E 120 COCHINEAL, CARMINIC ACID, CARMINES
Definition	Carmines and carminic acid are obtained from aqueous, aqueous alcoholic or alcoholic extracts from Cochineal, which consists of the dried bodies of the female insect Dactylopius coccus Costa. The colouring principle is carminic acid. Aluminium lakes of carminic acid (carmines) can be formed in which aluminium and carminic acid are thought to be present in the molar ratio 1:2. In commercial products the colouring principle is present in association with ammonium, calcium, potassium or sodium cations, singly or in combination, and these cations may also be present in excess. Commercial products may also contain proteinaceous material derived from the source insect, and may also contain free carminate or a small residue of unbound aluminium cations.
Class	Anthraquinone
Colour Index No	75470
Einecs	Cochineal: 215-680-6; carminic acid: 215-023-3; carmines: 215-724-4
Chemical names	7-β-D-glucopyranosyl-3,5,6,8-tetrahydroxy-1-methyl-9,10-dioxoanthracene-2-carboxylic acid (carminic acid); carmine is the hydrated aluminium chelate of this acid
Chemical formula	C22H20O13 (carminic acid)
Molecular weight	492,39 (carminic acid)
Assay	Content not less than 2,0 % carminic acid in the extracts containing carminic acid; not less than 50 % carminic acid in the chelates.
Description	Red to dark red, friable, solid or powder. Cochineal extract is generally a dark red liquid but can also be dried as a powder.
Identification
Spectrometry	Maximum in aqueous ammonia solution at ca 518 nm Maximum in dilute hydrochloric solution at ca 494 nm for carminic acid
Purity
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 122 AZORUBINE, CARMOISINE
Synonyms	CI Food Red 3
Definition	Azorubine consists essentially of disodium 4-hydroxy-3-(4-sulfonato-1-naphthylazo) naphthalene-1-sulfonate and subsidiary colouring matters together with sodium chloride and/or sodium sulfate as the principal uncoloured components. Azorubine is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Monoazo
Colour Index No	14720
Einecs	222-657-4
Chemical name	Disodium 4-hydroxy-3-(4-sulfonato-1-naphthylazo) naphthalene-1-sulfonate
Chemical formula	C20H12N2Na2O7S2
Molecular weight	502,44
Assay	Content not less than 85 % total colouring matters, calculated as the sodium salt E1 cm 1 % 510 at ca 516 nm in aqueous solution
Description	Red to maroon powder or granules
Identification
A. Spectrometry	Maximum in water at ca 516 nm
B. Red solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 2,0 %
Organic compounds other than colouring matters:
4-aminonaphthalene-1-sulfonic acid 4-hydroxynaphthalene-1-sulfonic acid	Total not more than 0,5 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	Not more than 0,2 % under neutral conditions
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 123 AMARANTH
Synonyms	CI Food Red 9
Definition	Amaranth consists essentially of trisodium 2-hydroxy-1-(4-sulfonato-1-naphthylazo) naphthalene-3,6-disulfonate and subsidiary colouring matters together with sodium chloride and/or sodium sulfate as the principal uncoloured components. Amaranth is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Monoazo
Colour Index No	16185
Einecs	213-022-2
Chemical name	Trisodium 2-hydroxy-1-(4-sulfonato-1-naphthylazo) naphthalene-3,6-disulfonate
Chemical formula	C20H11N2Na3O10S3
Molecular weight	604,48
Assay	Content not less than 85 % total colouring matters, calculated as the sodium salt E1 cm 1 % 440 at ca 520 nm in aqueous solution
Description	Reddish-brown powder or granules
Identification
A. Spectrometry	Maximum in water at ca 520 nm
B. Red solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 3,0 %
Organic compounds other than colouring matters:
4-aminonaphthalene-1-sulfonic acid 3-hydroxynaphthalene-2,7-disulfonic acid 6-hydroxynaphthalene-2-sulfonic acid 7-hydroxynaphthalene-1,3-disulfonic acid 7-hydroxynaphthalene-1,3-6-trisulfonic acid	Total not more than 0,5 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	Not more than 0,2 % under neutral conditions
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 124 PONCEAU 4R, COCHINEAL RED A
Synonyms	CI Food Red 7, New Coccine
Definition	Ponceau 4R consists essentially of trisodium 2-hydroxy-1-(4-sulfonato-1-naphthylazo) naphthalene-6,8-disulfonate and subsidiary colouring matters together with sodium chloride and/or sodium sulfate as the principal uncoloured components. Ponceau 4R is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Monoazo
Colour Index No	16255
Einecs	220-036-2
Chemical name	Trisodium 2-hydroxy-1-(4-sulfonato-1-naphthylazo) naphthalene-6,8-disulfonate
Chemical formula	C20H11N2Na3O10S3
Molecular weight	604,48
Assay	Content not less than 80 % total colouring matters, calculated as the sodium salt. E1 cm 1 % 430 at ca 505 nm in aqueous solution
Description	Reddish powder or granules
Identification
A. Spectrometry	Maximum in water at ca 505 nm
B. Red solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 1,0 %
Organic compounds other than colouring matters:
4-aminonaphthalene-1-sulfonic acid 7-hydroxynaphthalene-1,3-disulfonic acid 3-hydroxynaphthalene-2,7-disulfonic acid 6-hydroxynaphthalene-2-sulfonic acid 7-hydroxynaphthalene-1,3-6-trisulfonic acid	Total not more than 0,5 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	Not more than 0,2 % under neutral conditions
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 127 ERYTHROSINE
Synonyms	CI Food Red 14
Definition	Erythrosine consists essentially of disodium 2-(2,4,5,7-tetraiodo-3-oxido-6-oxoxanthen-9-yl) benzoate monohydrate and subsidiary colouring matters together with water, sodium chloride and/or sodium sulfate as the principal uncoloured components. Erythrosine is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Xanthen
Colour Index No	45430
Einecs	240-474-8
Chemical name	Disodium 2-(2,4,5,7-tetraiodo-3-oxido-6-oxoxanthen-9-yl)benzoate monohydrate
Chemical formula	C20H6I4Na2O5.H2O
Molecular weight	897,88
Assay	Content not less than 87 % total colouring matters, calculated as the anhydrous sodium salt E1 cm 1 %1 100 at ca 526 nm in aqueous solution at pH 7
Description	Red powder or granules.
Identification
A. Spectrometry	Maximum in water at ca 526 nm at pH 7
B. Red solution in water
Purity
Inorganic iodides calculated as sodium iodide	Not more than 0,1 %
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters (except fluorescein)	Not more than 4,0 %
Fluorescein	Not more than 20 mg/kg
Organic compounds other than colouring matters:
Tri-iodoresorcinol	Not more than 0,2 %
2-(2,4-dihydroxy-3,5-diodobenzoyl) benzoic acid	Not more than 0,2 %
Ether extractable matter	From a solution of pH from 7 through 8, not more than 0,2 %
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
Aluminium Lakes	The hydrochloric acid insoluble matter method is not applicable. It is replaced by a sodium hydroxide insoluble matter, at not more than 0,5 %, for this colour only.
E 128 RED 2G
Synonyms	CI Food Red 10, Azogeranine
Definition	Red 2G consists essentially of disodium 8-acetamido-1-hydroxy-2-phenylazonaphthalene-3,6-disulfonate and subsidiary colouring matters together with sodium chloride and/or sodium sulfate as the principal uncoloured components. Red 2G is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Monoazo
Colour Index No	18050
Einecs	223-098-9
Chemical name	Disodium 8-acetamido-1-hydroxy-2-phenylazo-naphthalene-3,6-disulfonate
Chemical formula	C18H13N3Na2O8S2
Molecular weight	509,43
Assay	Content not less than 80 % total colouring matters, calculated as the sodium salt E1 cm 1 % 620 at ca 532 nm in aqueous solution
Description	Red powder or granules
Identification
A. Spectrometry	Maximum in water at ca 532 nm
B. Red solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 2,0 %
Organic compounds other than colouring matters:
5-acetamido-4-hydroxynaphthalene-2,7-disulfonic acid 5-amino-4-hydroxynaphthalene-2,7-disulfonic acid	Total not more than 0,5 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	Not more than 0,2 % under neutral conditions
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 129 ALLURA RED AC
Synonyms	CI Food Red 17
Definition	Allura Red AC consists essentially of disodium 2-hydroxy-1-(2-methoxy-5-methyl-4-sulfonato-phenylazo) naphthalene-6-sulfonate and subsidiary colouring matters together with sodium chloride and/or sodium sulfate as the principal uncoloured components. Allura Red AC is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Monoazo
Colour Index No	16035
Einecs	247-368-0
Chemical name	Disodium 2-hydroxy-1-(2-methoxy-5-methyl-4-sulfonatophenylazo) naphthalene-6-sulfonate
Chemical formula	C18H14N2Na2O8S2
Molecular weight	496,42
Assay	Content not less than 85 % total colouring matters, calculated as the sodium salt E1 cm 1 % 540 at ca 504 nm in aqueous solution at pH 7
Description	Dark red powder or granules
Identification
A. Spectrometry	Maximum in water at ca 504 nm
B. Red solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 3,0 %
Organic compounds other than colouring matters:
6-hydroxy-2-naphthalene sulfonic acid, sodium salt	Not more than 0,3 %
4-amino-5-methoxy-2-methylbenezene sulfonic acid	Not more than 0,2 %
6,6-oxybis (2-naphthalene sulfonic acid) disodium salt	Not more than 1,0 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	From a solution of pH 7, not more than 0,2 %
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 131 PATENT BLUE V
Synonyms	CI Food Blue 5
Definition	Patent Blue V consists essentially of the calcium or sodium compound of [4-(α-(4-diethylaminophenyl)-5-hydroxy-2,4-disulfophenyl-methylidene)2,5-cyclohexadien-1-ylidene] diethylammonium hydroxide inner salt and subsidiary colouring matters together with sodium chloride and/or sodium sulfate and/or calcium sulfate as the principal uncoloured components. The potassium salt is also permitted.
Class	Triarylmethane
Colour Index No	42051
Einecs	222-573-8
Chemical names	The calcium or sodium compound of [4-(α-(4-diethylaminophenyl)-5-hydroxy-2,4-disulfophenyl-methylidene) 2,5-cyclohexadien-1-ylidene] diethyl-ammonium hydroxide inner salt
Chemical formula	Calcium compound: C27H31N2O7S2Ca Sodium compound: C27H31N2O7S2Na
Molecular weight	Calcium compound: 579,72 Sodium compound: 582,67
Assay	Content not less than 85 % total colouring matters, calculated as the sodium salt E1 cm 1 %2 000 at ca 638 nm in aqueous solution at pH 5
Description	Dark-blue powder or granules
Identification
A. Spectrometry	Maximum in water at 638 nm at pH 5
B. Blue solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 2,0 %
Organic compounds other than colouring matters:
3-hydroxy benzaldehyde 3-hydroxy benzoic acid 3-hydroxy-4-sulfobenzoic acid N,N-diethylamino benzene sulfonic acid	Total not more than 0,5 %
Leuco base	Not more than 4,0 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	From a solution of pH 5 not more than 0,2 %
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 132 INDIGOTINE, INDIGO CARMINE
Synonyms	CI Food Blue 1
Definition	Indigotine consists essentially of a mixture of disodium 3,3′dioxo-2,2′-bi-indolylidene-5,5′-disulfonate, and disodium 3,3′-dioxo-2,2′-bi-indolylidene-5,7′-disulfonate and subsidiary colouring matters together with sodium chloride and/or sodium sulfate as the principal uncoloured components. Indigotine is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Indigoid
Colour Index No	73015
Einecs	212-728-8
Chemical names	Disodium 3,3′-dioxo-2,2′-bi-indolylidene-5,5′-disulfonate
Chemical formula	C16H8N2Na2O8S2
Molecular weight	466,36
Assay	Content not less than 85 % total colouring matters, calculated as the sodium salt; disodium 3,3′-dioxo-2,2′-bi-indolylidene-5,7′-disulfonate: not more than 18 % E1 cm 1 % 480 at ca 610 nm in aqueous solution
Description	Dark-blue powder or granules
Identification
A. Spectrometry	Maximum in water at ca 610 nm
B. Blue solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Excluding disodium 3,3′-dioxo-2,2′-bi-indolylidene-5,7′-disulfonate: not more than 1,0 %
Organic compounds other than colouring matters:
Isatin-5-sulfonic acid 5-sulfoanthranilic acid Anthranilic acid	Total not more than 0,5 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	Not more than 0,2 % under neutral conditions
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 133 BRILLIANT BLUE FCF
Synonyms	CI Food Blue 2
Definition	Brilliant Blue FCF consists essentially of disodium α-(4-(N-ethyl-3-sulfonatobenzylamino) phenyl)-α-(4-N-ethyl-3-sulfonatobenzylamino) cyclohexa-2,5-dienylidene) toluene-2-sulfonate and its isomers and subsidiary colouring matters together with sodium chloride and/or sodium sulfate as the principal uncoloured components. Brilliant Blue FCF is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Triarylmethane
Colour Index No	42090
Einecs	223-339-8
Chemical names	Disodium α-(4-(N-ethyl-3-sulfonatobenzylamino) phenyl)-α-(4-N-ethyl-3-sulfonatobenzylamino) cyclohexa-2,5-dienylidene) toluene-2-sulfonate
Chemical formula	C37H34N2Na2O9S3
Molecular weight	792,84
Assay	Content not less than 85 % total colouring matters, calculated as the sodium salt E1 cm 1 %1 630 at ca 630 nm in aqueous solution
Description	Reddish-blue powder or granules
Identification
A. Spectrometry	Maximum in water at ca 630 nm
B. Blue solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 6,0 %
Organic compounds other than colouring matters:
Sum of 2-, 3- and 4-formyl benzene sulfonic acids	Not more than 1,5 %
3-((ethyl)(4-sulfophenyl) amino) methyl benzene sulfonic acid	Not more than 0,3 %
Leuco base	Not more than 5,0 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	Not more than 0,2 % at pH 7
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 140 (i) CHLOROPHYLLS
Synonyms	CI Natural Green 3, Magnesium Chlorophyll, Magnesium Phaeophytin
Definition	Chlorophylls are obtained by solvent extraction of natural strains of edible plant material, grass, lucerne and nettle. During the subsequent removal of solvent, the naturally present co-ordinated magnesium may be wholly or partly removed from the chlorophylls to give the corresponding phaeophytins. The principal colouring matters are the phaeophytins and magnesium chlorophylls. The extracted product, from which the solvent has been removed, contains other pigments such as carotenoids as well as oils, fats and waxes derived from the source material. Only the following solvents may be used for the extraction: acetone, methyl ethyl ketone, dichloromethane, carbon dioxide, methanol, ethanol, propan-2-ol and hexane.
Class	Porphyrin
Colour Index No	75810
Einecs	Chlorophylls: 215-800-7, chlorophyll a: 207-536-6, Chlorophyll b: 208-272-4
Chemical names	The major colouring principles are: Phytyl (132 R,17S,18S)-3-(8-ethyl-132-methoxycarbonyl-2,7,12,18-tetramethyl-13′-oxo-3-vinyl-131-132-17,18-tetrahydrocyclopenta [at]-porphyrin-17-yl)propionate, (Pheophytin a), or as the magnesium complex (Chlorophyll a) Phytyl (132 R,17S,18S)-3-(8-ethyl-7-formyl-132-methoxycarbonyl-2,12,18-trimethyl-13′-oxo-3-vinyl-131-132-17,18-tetrahydrocyclopenta[at]-porphyrin-17-yl)propionate, (Pheophytin b), or as the magnesium complex (Chlorophyll b)
Chemical formula	Chlorophyll a (magnesium complex): C55H72MgN4O5 Chlorophyll a: C55H74N4O5 Chlorophyll b (magnesium complex): C55H70MgN4O6 Chlorophyll b: C55H72N4O6
Molecular weight	Chlorophyll a (magnesium complex): 893,51 Chlorophyll a: 871,22 Chlorophyll b (magnesium complex): 907,49 Chlorophyll b: 885,20
Assay	Content of total combined Chlorophylls and their magnesium complexes is not less than 10 % E1 cm 1 % 700 at ca 409 nm in chloroform
Description	Waxy solid ranging in colour from olive green to dark green depending on the content of co-ordinated magnesium
Identification
Spectrometry	Maximum in chloroform at ca 409 nm
Purity
Solvent residues	Acetone Methyl Ethyl ketone Methanol Ethanol Propan-2-ol Hexane	Not more than 50 mg/kg, singly or in combination
	Dichloromethane: Not more than 10 mg/kg
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 140 (ii) CHLOROPHYLLINS
Synonyms	CI Natural Green 5, Sodium Chlorophyllin, Potassium Chlorophyllin
Definition	The alkali salts of chlorophyllins are obtained by the saponification of a solvent extract of natural strains of edible plant material, grass, lucerne and nettle. The saponification removes the methyl and phytol ester groups and may partially cleave the cyclopentenyl ring. The acid groups are neutralized to form the salts of potassium and/or sodium. Only the following solvents may be used for the extraction: acetone, methyl ethyl ketone, dichloromethane, carbon dioxide, methanol, ethanol, propan-2-ol and hexane.
Class	Porphyrin
Colour Index No	75815
Einecs	287-483-3
Chemical names	The major colouring principles in their acid forms are: 3-(10-carboxylato-4-ethyl-1,3,5,8-tetramethyl-9-oxo-2-vinylphorbin-7-yl)propionate (chlorophyllin a) and 3-(10-carboxylato-4-ethyl-3-formyl-1,5,8-trimethyl-9-oxo-2-vinylphorbin-7-yl)propionate (chlorophyllin b) Depending on the degree of hydrolysis the cyclopentenyl ring may be cleaved with the resultant production of a third carboxyl function. Magnesium complexes may also be present.
Chemical formula	Chlorophyllin a (acid form): C34H34N4O5 Chlorophyllin b (acid form): C34H32N4O6
Molecular weight	Chlorophyllin a: 578,68 Chlorophyllin b: 592,66 Each may be increased by 18 daltons if the cyclopentenyl ring is cleaved.
Assay	Content of total chlorophyllins is not less than 95 % of the sample dried at ca 100 °C for 1 hour. E1 cm 1 % 700 at ca 405 nm in aqueous solution at pH 9 E1 cm 1 % 140 at ca 653 nm in aqueous solution at pH 9
Description	Dark green to blue/black powder
Identification
Spectrometry	Maximum in aqueous phosphate buffer at pH 9 at ca 405 nm and at ca 653 nm
Purity
Solvent residues	Acetone Methyl ethyl ketone Methanol Ethanol Propan-2-ol Hexane	Not more than 50 mg/kg, singly or in combination
	Dichloromethane: not more than 10 mg/kg
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 141 (i) COPPER COMPLEXES OF CHLOROPHYLLS
Synonyms	CI Natural Green 3, Copper Chlorophyll, Copper Phaeophytin
Definition	Copper chlorophylls are obtained by addition of a salt of copper to the substance obtained by solvent extraction of natural strains of edible plant material, grass, lucerne, and nettle. The product, from which the solvent has been removed, contains other pigments such as carotenoids as well as fats and waxes derived from the source material. The principal colouring matters are the copper phaeophytins. Only the following solvents may be used for the extraction: acetone, methyl ethyl ketone, dichloromethane, carbon dioxide, methanol, ethanol, propan-2-ol and hexane.
Class	Porphyrin
Colour Index No	75815
Einecs	Copper chlorophyll a: 239-830-5; copper chlorophyll b: 246-020-5
Chemical names	[Phytyl (132 R,17S,18S)-3-(8-ethyl-132-methoxycarbonyl-2,7,12,18-tetramethyl-13′-oxo-3-vinyl-131-132-17,18-tetrahydrocyclopenta[at]-porphyrin-17-yl)propionate] copper (II) (Copper Chlorophyll a) [Phytyl (132 R,17S,18S)-3-(8-ethyl-7-formyl-132-methoxycarbonyl-2,12,18-trimethyl-13′-oxo-3-vinyl-131-132-17,18-tetrahydrocyclopenta[at]-porphyrin-17-yl)propionate] copper (II) (Copper chlorophyll b)
Chemical formula	Copper chlorophyll a: C55H72Cu N4O5 Copper chlorophyll b: C55H70Cu N4O6
Molecular weight	Copper chlorophyll a: 932,75 Copper chlorophyll b: 946,73
Assay	Content of total copper chlorophylls is not less than 10 %. E1 cm 1 % 540 at ca 422 nm in chloroform E1 cm 1 % 300 at ca 652 nm in chloroform
Description	Waxy solid ranging in colour from blue green to dark green depending on the source material
Identification
Spectrometry	Maximum in chloroform at ca 422 nm and at ca 652 nm
Purity
Solvent residues	Acetone Methyl ethyl ketone Methanol Ethanol Propan-2-ol Hexane	Not more than 50 mg/kg, singly or in combination
	Dichloromethane: not more than 10 mg/kg
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Copper ions	Not more than 200 mg/kg
Total copper	Not more than 8,0 % of the total copper phaeophytins
E 141 (ii) COPPER COMPLEXES OF CHLOROPHYLLINS
Synonyms	Sodium Copper Chlorophyllin, Potassium Copper Chlorophyllin, CI Natural Green 5
Definition	The alkali salts of copper chlorophyllins are obtained by the addition of copper to the product obtained by the saponification of a solvent extraction of natural strains of edible plant material, grass, lucerne, and nettle; the saponification removes the methyl and phytol ester groups and may partially cleave the cyclopentenyl ring. After addition of copper to the purified chlorophyllins, the acid groups are neutralized to form the salts of potassium and/or sodium. Only the following solvents may be used for the extraction: acetone, methyl ethyl ketone, dichloromethane, carbon dioxide methanol, ethanol, propan-2-ol and hexane.
Class	Porphyrin
Colour Index No	75815
Einecs
Chemical names	The major colouring principles in their acid forms are: 3-(10-Carboxylato-4-ethyl-1,3,5,8-tetramethyl-9-oxo-2-vinylphorbin-7-yl)propionate, copper complex (Copper chlorophyllin a) and 3-(10-Carboxylato-4-ethyl-3-formyl-1,5,8-trimethyl-9-oxo-2-vinylphorbin-7-yl) propionate, copper complex (Copper chlorophyllin b)
Chemical formula	Copper chlorophyllin a (acid form): C34H32Cu N4O5 Copper chlorophyllin b (acid form): C34H30Cu N4O6
Molecular weight	Copper chlorophyllin a: 640,20 Copper chlorophyllin b: 654,18 Each may be increased by 18 daltons if the cyclopentenyl ring is cleaved.
Assay	Content of total copper chlorophyllins is not less than 95 % of the sample dried at 100 °C for 1 h. E1 cm 1 % 565 at ca 405 nm in aqueous phosphate buffer at pH 7,5 E1 cm 1 % 145 at ca 630 nm in aqueous phosphate buffer at pH 7,5
Description	Dark green to blue/black powder
Identification
Spectrometry	Maximum in aqueous phosphate buffer at pH 7,5 at ca 405 nm and at ca 630 nm
Purity
Solvent residues	Acetone Methyl ethyl ketone Methanol Ethanol Propan-2-ol Hexane	Not more than 50 mg/kg, singly or in combination
	Dichloromethane: not more than 10 mg/kg
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Copper ions	Not more than 200 mg/kg
Total copper	Not more than 8,0 % of the total copper chlorophyllins
E 142 GREEN S
Synonyms	CI Food Green 4, Brilliant Green BS
Definition	Green S consists essentially of sodium N-[4-(dimethylamino)phenyl] 2-hydroxy-3,6-disulfo-1-naphthalenyl)methylene]-2,5-cyclohexadien-1-ylidene]-N-methylmethanaminium and subsidiary colouring matters together with sodium chloride and/or sodium sulphate as the principal uncoloured compounds. Green S is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Triarylmethane
Colour Index No	44090
Einecs	221-409-2
Chemical names	Sodium N-[4-[[4-(dimethylamino)phenyl](2-hydroxy-3,6-disulfo-1-naphthalenyl)-methylene]2,5-cyclohexadien-1-ylidene]-N-methylmethanaminium; Sodium 5-[4-dimethylamino-α-(4-dimethyliminocyclohexa-2,5-dienylidene) benzyl]-6-hydroxy-7-sulfonato-naphthalene-2-sulfonate (alternative chemical name).
Chemical formula	C27H25N2NaO7S2
Molecular Weight	576,63
Assay	Content not less than 80 % total colouring matters calculated as the sodium salt E1 cm 1 %1 720 at ca 632 nm in aqueous solution
Description	Dark blue or dark green powder or granules
Identification
A. Spectrometry	Maximum in water at ca 632 nm
B. Blue or green solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 1,0 %
Organic compounds other than colouring matters:
4,4′-bis(dimethylamino)-benzhydryl alcohol	Not more than 0,1 %
4,4′-bis(dimethylamino)-benzophenone	Not more than 0,1 %
3-hydroxynaphthalene-2,7-disulfonic acid	Not more than 0,2 %
Leuco base	Not more than 5,0 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	Not more than 0,2 % under neutral conditions
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 150a PLAIN CARAMEL
Definition	Plain caramel is prepared by the controlled heat treatment of carbohydrates (commercially available food grade nutritive sweeteners which are the monomers glucose and fructose and/or polymers thereof, e.g. glucose syrups, sucrose, and/or invert syrups, and dextrose). To promote caramelization, acids, alkalis and salts may be employed, with the exception of ammonium compounds and sulphites.
Einecs	232-435-9
Description	Dark brown to black liquids or solids
Purity
Colour bound by DEAE cellulose	Not more than 50 %
Colour bound by phosphoryl cellulose	Not more than 50 %
Colour intensitya	0,01-0,12
Total nitrogen	Not more than 0,1 %
Total sulphur	Not more than 0,2 %
Arsenic	Not more than 1 mg/kg
Lead	Not more than 2 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 25 mg/kg
E 150b CAUSTIC SULPHITE CARAMEL
Definition	Caustic sulphite caramel is prepared by the controlled heat treatment of carbohydrates (commercially available food grade nutritive sweeteners which are the monomers glucose and fructose and/or polymers thereof, e.g. glucose syrups, sucrose, and/or invert syrups, and dextrose) with or without acids or alkalis, in the presence of sulphite compounds (sulphurous acid, potassium sulphite, potassium bisulphite, sodium sulphite and sodium bisulphite); no ammonium compounds are used.
Einecs	232-435-9
Description	Dark brown to black liquids or solids
Purity
Colour bound by DEAE cellulose	More than 50 %
Colour intensitya	0,05-0,13
Total nitrogen	Not more than 0,3 %b
Sulphur dioxide	Not more than 0,2 %b
Total sulphur	0,3-3,5 %b
Sulphur bound by DEAE cellulose	More than 40 %
Absorbance ratio of colour bound by DEAE cellulose	19-34
Absorbance ratio (A 280/560)	Greater than 50
Arsenic	Not more than 1 mg/kg
Lead	Not more than 2 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 25 mg/kg
E 150c AMMONIA CARAMEL
Definition	Ammonia caramel is prepared by the controlled heat treatment of carbohydrates (commercially available food grade nutritive sweeteners which are the monomers glucose and fructose and/or polymers thereof, e.g. glucose syrups, sucrose, and/or invert syrups, and dextrose) with or without acids or alkalis, in the presence of ammonium compounds (ammonium hydroxide, ammonium carbonate, ammonium hydrogen carbonate and ammonium phosphate); no sulphite compounds are used.
Einecs	232-435-9
Description	Dark brown to black liquids or solids
Purity
Colour bound by DEAE cellulose	Not more than 50 %
Colour bound by phosphoryl cellulose	More than 50 %
Colour intensitya	0,08-0,36
Ammoniacal nitrogen	Not more than 0,3 %b
4-methylimidazole	Not more than 250 mg/kgb
2-acetyl-4-tetrahydroxy-butylimidazole	Not more than 10 mg/kgb
Total sulphur	Not more than 0,2 %b
Total nitrogen	0,7-3,3 %b
Absorbance ratio of colour bound by phosphoryl cellulose	13-35
Arsenic	Not more than 1 mg/kg
Lead	Not more than 2 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 25 mg/kg
E 150d SULPHITE AMMONIA CARAMEL
Definition	Sulphite ammonia caramel is prepared by the controlled heat treatment of carbohydrates (commercially available food grade nutritive sweeteners which are the monomers glucose and fructose and/or polymers thereof (e.g. glucose syrups, sucrose, and/or invert syrups, and dextrose) with or without acids or alkalis in the presence of both sulphite and ammonium compounds (sulphurous acid, potassium sulphite, potassium bisulphite, sodium sulphite, sodium bisulphite, ammonium hydroxide, ammonium carbonate, ammonium hydrogen carbonate, ammonium phosphate, ammonium sulphate, ammonium sulphite and ammonium hydrogen sulphite).
Einecs	232-435-9
Description	Dark brown to black liquids or solids
Purity
Colour bound by DEAE cellulose	More than 50 %
Colour intensitya	0,10-0,60
Ammoniacal nitrogen	Not more than 0,6 %b
Sulphur dioxide	Not more than 0,2 %b
4-methylimidazole	Not more than 250 mg/kgb
Total nitrogen	0,3-1,7 %b
Total sulphur	0,8-2,5 %b
Nitrogen/sulphur ratio of alcohol precipitate	0,7-2,7
Absorbance ratio of alcohol precipitatec	8-14
Absorbance ratio (A 280/560)	Not more than 50
Arsenic	Not more than 1 mg/kg
Lead	Not more than 2 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 25 mg/kg
E 151 BRILLIANT BLACK BN, BLACK PN
Synonyms	CI Food Black 1
Definition	Brilliant Black BN consists essentially of tetrasodium-4-acetamido-5-hydroxy-6-[7-sulfonato-4-(4-sulfonatophenylazo)-1-naphthylazo] naphthalene-1,7-disulfonate and subsidiary colouring matters together with sodium chloride and/or sodium sulfate as the principal uncoloured components. Brilliant Black BN is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Bisazo
Colour Index No	28440
Einecs	219-746-5
Chemical names	Tetrasodium 4-acetamido-5-hydroxy-6-[7-sulfonato-4-(4-sulfonatophenylazo)-1-naphthylazo] naphthalene-1,7-disulfonate
Chemical formula	C28H17N5Na4O14S4
Molecular weight	867,69
Assay	Content not less than 80 % total colouring matters calculated as the sodium salt E1 cm 1 % 530 at ca 570 nm in solution
Description	Black powder or granules
Identification
A. Spectrometry	Maximum in water at ca 570 nm
B. Black-bluish solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 10 % (expressed on the dye content)
Organic compounds other than colouring matters:
4-acetamido-5-hydroxynaphthalene-1,7-disulfonic acid 4-amino-5-hydroxynaphthalene-1,7-disulfonic acid 8-aminonaphthalene-2-sulfonic acid 4,4′-diazoaminodi-(benzenesulfonic acid)	Total not more than 0,8 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	Not more than 0,2 % under neutral conditions
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 153 VEGETABLE CARBON
Synonyms	Vegetable black
Definition	Vegetable carbon is produced by the carbonization of vegetable material such as wood, cellulose residues, peat and coconut and other shells. The raw material is carbonised at high temperatures. It consists essentially of finely divided carbon. It may contain minor amounts of nitrogen, hydrogen and oxygen. Some moisture may be absorbed on the product after manufacture.
Colour Index No	77266
Einecs	215-609-9
Chemical names	Carbon
Chemical formula	C
Molecular weight	12,01
Assay	Content not less than 95 % of carbon calculated on an anhydrous and ash-free basis
Description	Black powder, odourless and tasteless
Identification
A. Solubility	Insoluble in water and organic solvents
B. Burning	When heated to redness it burns slowly without a flame
Purity
Ash (Total)	Not more than 4,0 % (ignition temperature: 625 °C)
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
Polyaromatic hydrocarbons	The extract obtained by extraction of 1 g of the product with 10 g pure cyclohexane in a continuous extraction apparatus shall be colourless, and the fluorescence of the extract in ultraviolet light shall not be more intense than that of a solution of 0,100 mg of quinine sulfate in 1 000 ml of 0,01 M sulphuric acid.
Loss on drying	Not more than 12 % (120 °C, 4 hrs)
Alkali soluble matter	The filtrate obtained by boiling 2 g of the sample with 20 ml N sodium hydroxide and filtering shall be colourless
E 154 BROWN FK
Synonyms	CI Food Brown 1
Definition	Brown FK consists essentially of a mixture of: I sodium 4-(2,4-diaminophenylazo) benzenesulfonate II sodium 4-(4,6-diamino-m-tolylazo) benzenesulfonate III disodium 4,4′-(4,6-diamino-1,3-phenylenebisazo)di (benzenesulfonate) IV disodium 4,4′-(2,4-diamino-1,3-phenylenebisazo)di (benzenesulfonate) V disodium 4,4′-(2,4-diamino-5-methyl-1,3-phenylenebisazo)di (benzenesulfonate) VI trisodium 4,4′,4″-(2,4-diaminobenzene-1,3,5-trisazo)tri-(benzenesulfonate) and subsidiary colouring matters together with water, sodium chloride and/or sodium sulfate as the principal uncoloured components. Brown FK is described as the sodium salt. The calcium and the potassium salt are also permitted.
Class	Azo (a mixture of mono-, bis- and trisazo colours)
Einecs
Chemical names	A mixture of: I sodium 4-(2,4-diaminophenylazo) benzenesulfonate II sodium 4-(4,6-diamino-m-tolylazo) benzenesulfonate III disodium 4,4′-(4,6-diamino-1,3-phenylenebisazo)di (benzenesulfonate) IV disodium 4,4′-(2,4-diamino-1,3-phenylenebisazo)di (benzenesulfonate) V disodium 4,4′-(2,4-diamino-5-methyl-1,3-phenylenebisazo)di (benzenesulfonate) VI trisodium 4,4′,4″-(2,4-diaminobenzene-1,3,5-trisazo)tri-(benzenesulfonate)
Chemical formula	I C12H11N4NaO3S II C13H13N4NaO3S III C18H14N6Na2O6S2 IV C18H14N6Na2O6S2 V C19H16N6Na2O6S2 VI C24H17N8Na3O9S3
Molecular weight	I 314,30 II 328,33 III 520,46 IV 520,46 V 534,47 VI 726,59
Assay	Content not less than 70 % total colouring matters Of the total colouring matters present the proportions of the components shall not exceed: I 26 % II 17 % III 17 % IV 16 % V 20 % VI 16 %
Description	Red-brown powder or granules
Identification
Orange to reddish solution
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 3,5 %
Organic compounds other than colouring matters:
4-aminobenzene-1-sulfonic acid	Not more than 0,7 %
m-phenylenediamine and 4-methyl-m-phenylenediamine	Not more than 0,35 %
Unsulfonated primary aromatic amines other than m-phenylene diamine and 4-methyl-m-phenylene diamine	Not more than 0,007 % (calculated as aniline)
Ether extractable matter	From a solution of pH 7, not more than 0,2 %
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 155 BROWN HT
Synonyms	CI Food Brown 3
Definition	Brown HT consists essentially of disodium 4,4′-(2,4-dihydroxy-5-hydroxymethyl-1,3-phenylene bisazo) di (naphthalene-1-sulfonate) and subsidiary colouring matters together with sodium chloride and/or sulfate as the principal uncoloured components. Brown HT is described as the sodium salt. The calcium and potassium salt are also permitted.
Class	Bisazo
Colour Index No	20285
Einecs	224-924-0
Chemical names	Disodium 4,4′-(2,4-dihydroxy-5-hydroxymethyl-1,3-phenylene bisazo)di (naphthalene-1-sulfonate)
Chemical formula	C27H18N4Na2O9S2
Molecular Weight	652,57
Assay	Content not less than 70 % total colouring matters calculated as the sodium salt. E1 cm 1 % 403 at ca 460 nm in aqueous solution at pH 7
Description	Reddish-brown powder or granules
Identification
A. Spectrometry	Maximum in water of pH 7 at ca 460 nm
B. Brown solution in water
Purity
Water insoluble matter	Not more than 0,2 %
Subsidiary colouring matters	Not more than 10 % (TLC method)
Organic compounds other than colouring matters:
4-aminonaphthalene-1-sulfonic acid	Not more than 0,7 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (calculated as aniline)
Ether extractable matter	Not more than 0,2 % in a solution of pH 7
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 160a (i) MIXED CAROTENES
1. Plant carotenes
Synonyms	CI Food Orange 5
Definition	Mixed carotenes are obtained by solvent extraction of natural strains of edible plants, carrots, vegetable oils, grass, alfalfa (lucerne) and nettle. The main colouring principle consists of carotenoids of which beta-carotene accounts for the major part. Alpha, gamma-carotene and other pigments may be present. Besides the colour pigments, this substance may contain oils, fats and waxes naturally occurring in the source material. Only the following solvents may be used in the extraction: acetone, methyl ethyl ketone, methanol, ethanol, propan-2-ol, hexaned, dichloromethane and carbon dioxide.
Class	Carotenoid
Colour Index No	75130
Einecs	230-636-6
Chemical formula	Beta-carotene: C40H56
Molecular weight	Beta-carotene: 536,88
Assay	Content of carotenes (calculated as beta-carotene) is not less than 5 %. For products obtained by extraction of vegetables oils: not less than 0,2 % in edible fats. E1 cm 1 %2 500 at approximately 440 nm to 457 nm in cyclohexane
Identification
Spectrometry	Maximum in cyclohexane at 440 nm to 457 nm and 470 nm to 486 nm
Purity
Solvent residues	Acetone Methyl ethyl ketone Methanol Propan-2-ol Hexane Ethanol	Not more than 50 mg/kg, singly or in combination
	Dichloromethane: Not more than 10 mg/kg
Lead	Not more than 5 mg/kg
2. Algal carotenes
Synonyms	CI Food Orange 5
Definition	Mixed carotenes may also be produced from natural strains of the algae Dunaliella salina, grown in large saline lakes located in Whyalla, South Australia. Beta-carotene is extracted using an essential oil. The preparation is a 20 to 30 % suspension in edible oil. The ratio of trans-cis isomers is in the range of 50/50 to 71/29. The main colouring principle consists of carotenoids of which beta-carotene accounts for the major part. Alpha-carotene, lutein, zeaxanthin and beta-cryptoxanthin may be present. Besides the colour pigments, this substance may contain oils, fats and waxes naturally occurring in the source material.
Class	Carotenoid
Colour Index No	75130
Chemical formula	Beta-Carotene: C40H56
Molecular weight	Beta-Carotene: 536,88
Assay	Content of carotenes (calculated as beta-carotene) is not less than 20 % E1 cm 1 %2 500 at approximately by 440 nm to 457 nm in cyclohexane
Identification
Spectrometry	Maximum in cyclohexane at 440 nm to 457 nm and 474 nm to 486 nm
Purity
Natural tocopherols in edible oil	Not more than 0,3 %
Lead	Not more than 5 mg/kg
E 160a (ii) BETA-CAROTENE
1. Beta-carotene
Synonyms	CI Food Orange 5
Definition	These specifications apply predominantly to all trans isomer of beta-carotene together with minor amounts of other carotenoids. Diluted and stabilised preparations may have different trans-cis isomer ratios.
Class	Carotenoid
Colour Index No	40800
Einecs	230-636-6
Chemical names	Beta-carotene, beta, beta-carotene
Chemical formula	C40H56
Molecular weight	536,88
Assay	Not less than 96 % total colouring matters (expressed as beta-carotene) E1 cm 1 %2 500 at approximately by 440 nm to 457 nm in cyclohexane
Description	Red to brownish-red crystals or crystalline powder
Identification
Spectrometry	Maximum in cyclohexane at 453 nm to 456 nm
Purity
Sulfated ash	Not more than 0,2 %
Subsidiary colouring matters	Carotenoids other than beta-carotene: not more than 3,0 % of total colouring matters
Lead	Not more than 2 mg/kg
2. Beta-carotene from Blakeslea trispora
Synonyms	CI Food Orange 5
Definition	Obtained by a fermentation process using a mixed culture of the two sexual mating types (+) and (–) of natural strains of the fungus Blakeslea trispora. The beta-carotene is extracted from the biomass with ethyl acetate, or isobutyl acetate followed by isopropyl alcohol, and crystallised. The crystallised product consists mainly of trans beta-carotene. Because of the natural process approximately 3 % of the product consists of mixed carotenoids, which is specific for the product.
Class	Carotenoid
Colour Index No	40800
Einecs	230-636-6
Chemical names	Beta-carotene, beta,beta-carotene
Chemical formula	C40H56
Molecular weight	536,88
Assay	Not less than 96 % total colouring matters (expressed as beta-carotene) E1 cm 1 %2 500 at approximately 440 nm to 457 nm in cyclohexane
Description	Red, brownish-red or purple-violet crystals or crystalline powder (colour varies according to extraction solvent used and conditions of crystallisation)
Identification
Spectrometry	Maximum in cyclohexane at 453 nm to 456 nm
Purity
Solvent residues	Ethyl acetate Ethanol	Not more than 0,8 %, singly or in combination
	Isobutyl acetate: Not more than 1,0 %
	Isopropyl alcohol: Not more than 0,1 %
Sulfated ash	Not more than 0,2 %
Subsidiary colouring matters	Carotenoids other than beta-carotene: not more than 3,0 % of total colouring matters
Lead	Not more than 2 mg/kg
Mycotoxins:
Aflatoxin B1	Absent
Trichothecene (T2)	Absent
Ochratoxin	Absent
Zearalenone	Absent
Microbiology:
Moulds	Not more than 100/g
Yeasts	Not more than 100/g
Salmonella	Absent in 25 g
Escherichia coli	Absent in 5 g
E 160b ANNATTO, BIXIN, NORBIXIN
Synonyms	CI Natural Orange 4
Definition
Class	Carotenoid
Colour Index No	75120
Einecs	Annatto: 215-735-4, annatto seed extract: 289-561-2; bixin: 230-248-7
Chemical names	Bixin : 6′-Methylhydrogen-9′-cis-6,6′-diapocarotene-6,6′-dioate 6′-Methylhydrogen-9′-trans-6,6′-diapocarotene-6,6′-dioate Norbixin : 9′Cis-6,6′-diapocarotene-6,6′-dioic acid 9′-Trans-6,6′-diapocarotene-6,6′-dioic acid
Chemical formula	Bixin : C25H30O4 Norbixin : C24H28O4
Molecular weight	Bixin : 394,51 Norbixin : 380,48
Description	Reddish-brown powder, suspension or solution
Identification
Spectrometry	Bixin : maximum in chloroform at ca 502 nm Norbixin : maximum in dilute KOH solution at ca 482 nm
(i) Solvent extracted bixin and norbixin
Definition	Bixin is prepared by the extraction of the outer coating of the seeds of the annatto tree (Bixa orellana L.) with one or more of the following solvents: acetone, methanol, hexane or dichloromethane, carbon dioxide followed by the removal of the solvent. Norbixin is prepared by hydrolysis by aqueous alkali of the extracted bixin. Bixin and norbixin may contain other materials extracted from the annatto seed. The bixin powder contains several coloured components, the major single one being bixin, which may be present in both cis- and trans- forms. Thermal degradation products of bixin may also be present. The norbixin powder contains the hydrolysis product of bixin, in the form of the sodium or potassium salts as the major colouring principle. Both cis- and trans-forms may be present.
Assay	Content of bixin powders not less than 75 % total carotenoids calculated as bixin. Content of norbixin powders not less than 25 % total carotenoids calculated as norbixin Bixin : E1 cm 1 %2 870 at ca 502 nm in chloroform Norbixin : E1 cm 1 %2 870 at ca 482 nm in KOH solution
Purity
Solvent residues	Acetone Methanol Hexane	not more than 50 mg/kg, singly or in combination
	Dichloromethane: not more than 10 mg/kg
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
(ii) Alkali extracted annatto
Definition	Water soluble annatto is prepared by extraction with aqueous alkali (sodium or potassium hydroxide) of the outer coating of the seeds of the annatto tree (Bixa orellana L.) Water soluble annatto contains norbixin, the hydrolysis product of bixin, in the form of the sodium or potassium salts, as the major colouring principle. Both cis- and trans- forms may be present.
Assay	Contains not less than 0,1 % of total carotenoids expressed as norbixin Norbixin : E1 cm 1 %2 870 at ca 482 nm in KOH solution
Purity
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
(iii) Oil extracted annatto
Definition	Annatto extracts in oil, as solution or suspension, are prepared by extraction of the outer coating of the seeds of the annatto tree (Bixa orellana L.) with edible vegetable oil. Annatto extract in oil contains several coloured components, the major single one being bixin, which may be present in both cis- and transforms. Thermal degradation products of bixin may also be present.
Assay	Contains not less than 0,1 % of total carotenoids expressed as bixin Bixin : E1 cm 1 %2 870 at ca 502 nm in chloroform
Purity
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 160c PAPRIKA EXTRACT, CAPSANTHIN, CAPSORUBIN
Synonyms	Paprika Oleoresin
Definition	Paprika extract is obtained by solvent extraction of the natural strains of paprika, which consists of the ground fruits pods, with or without seeds, of Capsicum annuum L., and contains the major colouring principles of this spice. The major colouring principles are capsanthin and capsorubin. A wide variety of other coloured compounds is known to be present. Only the following solvents may be used in the extraction: methanol, ethanol, acetone, hexane, dichloromethane, ethyl acetate and carbon dioxide.
Class	Carotenoid
Einecs	Capsanthin: 207-364-1, capsorubin: 207-425-2
Chemical names	Capsanthin: (3R, 3′S, 5′R)-3,3′-dihydroxy-β,k-carotene-6-one Capsorubin: (3S, 3′S, 5R, 5R′)-3,3′-dihydroxy-k,k-carotene-6,6′-dione
Chemical formula	Capsanthin: C40H56O3 Capsorubin: C40H56O4
Molecular weight	Capsanthin: 584,85 Capsorubin: 600,85
Assay	Paprika extract: content not less than 7,0 % carotenoids Capsanthin/capsorubin: not less than 30 % of total carotenoids E1 cm 1 %2 100 at ca 462 nm in acetone
Description	Dark-red viscous liquid
Identification
A. Spectrometry	Maximum in acetone at ca 462 nm
B. Colour reaction	A deep blue colour is produced by adding one drop of sulfuric acid to one drop of sample in 2-3 drops of chloroform
Purity
Solvent residues	Ethyl acetate Methanol Ethanol Acetone Hexane	Not more than 50 mg/kg, singly or in combination
	Dichloromethane: not more than 10 mg/kg
Capsaicin	Not more than 250 mg/kg
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
[F1 E 160 D LYCOPENE
(i) synthetic lycopene
Synonyms	Lycopene from chemical synthesis
Definition	Synthetic lycopene is a mixture of geometric isomeres of lycopenes and is produced by the Wittig condensation of synthetic intermediates commonly used in the production of other carotenoids used in food. Synthetic lycopene consists predominantly of all-trans-lycopene together with 5-cis-lycopene and minor quantities of other isomers. Commercial lycopene preparations intended for use in food are formulated as suspensions in edible oils or water-dispersible or water- soluble powder.
Colour Index No	75125
EINECS	207-949-1
Chemical name	Ψ,Ψ-carotene, all-trans-lycopene, (all-E)-lycopene, (all-E)-2,6,10,14,19,23,27,31-octamethyl-2,6,8,10,12,14,16,18,20,22,24,26,30-dotriacontatridecaene
Chemical formula	C 40 H 56
Molecular weight	536,85
Assay	Not less than 96 % total lycopenes (not less than 70 % all-translycopene) E 1 cm 1 % at 465 - 475 nm in hexane (for 100 % pure all-translycopene) is 3 450
Description	Red crystalline powder
Identification
Spectrophotometry	A solution in hexane shows an absorption maximum at approximately 470 nm
Test for carotenoids	The colour of the solution of the sample in acetone disappears after successive additions of a 5 % solution of sodium nitrite and 1N sulphuric acid
Solubility	Insoluble in water, freely soluble in chloroform
Properties of 1 % solution in chloroform	Is clear and has intensive red-orange colour
Purity
Loss on drying	Not more than 0,5 % (40 °C, 4 h at 20 mm Hg)
Apo-12’-lycopenal	Not more than 0,15 %
Triphenyl phosphine oxide	Not more than 0,01 %
Solvent residues	Methanol not more than 200 mg/kg, Hexane, Propan-2-ol: Not more than 10 mg/kg each. Dichloromethane: Not more than 10 mg/kg (in commercial preparations only)
Lead	Not more than 1 mg/kg
(ii) from red tomatoes
Synonyms	Natural Yellow 27
Definition	Lycopene is obtained by solvent extraction of red tomatoes ( Lycopersicon esculentum L.) with subsequent removal of the solvent. Only the following solvent may be used: carbon dioxide, ethyl acetate, acetone, propan-2-ol, methanol, ethanol, hexane. The major colouring principle of tomatoes is lycopene, minor amounts of other carotenoid pigments may be present. Besides the colour pigments the product may contain oil, fats, waxes and flavour components naturally occurring in tomatoes.
Colour Index No	75125
EINECS	207-949-1
Chemical name	Ψ,Ψ-carotene, all-trans-lycopene, (all-E)-lycopene, (all-E)-2,6,10,14,19,23,27,31-octamethyl-2,6,8,10,12,14,16,18,20,22,24,26,30-dotriacontatridecaene
Chemical formula	C 40 H 56
Molecular weight	536,85
Assay	E 1 cm 1 % at 465 - 475 nm in hexane (for 100 % pure all-translycopene) is 3 450 . Content not less than 5 % total colouring matters
Description	Dark red viscous liquid
Identification
Spectrophotometry	Maximum in hexane at ca 472 nm
Purity
Solvent residues	Propane-2-ol Hexane Acetone Ethanol Methanol Ethylacetate Not more than 50 mg/kg, singly or in combination
Sulphated ash	Not more than 1 %
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Arsenic	Not more than 3 mg/kg
Lead	Not more than 2 mg/kg
(iii) from Blakeslea trispora
Synonyms	Natural Yellow 27
Definition	Lycopene from Blakeslea trispora is extracted from the fungal biomass and purified by crystallisation and filtration. It consists predominantly of all-trans-lycopene. It also contains minor quantities of other carotenoids. Isopropanol and isobutyl acetate are the only solvents used in the manufacture. Commercial lycopene preparations intended for use in food are formulated as suspensions in edible oils or water-dispersible or water-soluble powder.
Colour Index No	75125
EINECS	207-949-1
Chemical name	Ψ,Ψ-carotene, all-trans-lycopene, (all-E)-lycopene, (all-E)-2,6,10,14,19,23,27,31-octamethyl-2,6,8,10,12,14,16,18,20,22,24,26,30-dotriacontatridecaene
Chemical formula	C 40 H 56
Molecular weight	536,85
Assay	Not less than 95 % total lycopenes and not less than 90 % all-translycopene of all colouring matters E 1 cm 1 % at 465 - 475 nm in hexane (for 100 % pure all-translycopene) is 3 450
Description	Red crystalline powder
Identification
Spectrophotometry	A solution in hexane shows an absorption maximum at approximately 470 nm
Test of carotenoids	The colour of the solution of the sample in acetone disappears after successive additions of a 5 % solution of sodium nitrite and 1N sulphuric acid
Solubility	Insoluble in water, freely soluble in chloroform
Properties of 1 % solution in chloroform	Is clear and has intensive red-orange colour
Purity
Loss on drying	Not more than 0,5 % (40 °C, 4 h at 20 mm Hg)
Other carotenoids	Not more than 5 %
Solvent residues	Propan-2-ol: Not more than 0,1 % Isobutyl acetate: Not more than 1,0 % Dichloromethane: Not more than 10 mg/kg (in commercial preparations only)
Sulphated ash	Not more than 0,3 %
Lead	Not more than 1 mg/kg]
E 160e BETA-APO-8′-CAROTENAL (C30)
Synonyms	CI Food Orange 6
Definition	These specifications apply to predominantly all trans isomer of β-apo-8′-carotenal together with minor amounts of other carotenoids. Diluted and stabilized forms are prepared from β-apo-8′-carotenal meeting these specifications and include solutions or suspensions of ß-apo-8′carotenal in edible fats or oils, emulsions and water dispersible powders. These preparations may have different cis/trans isomer ratios.
Class	Carotinoid
Colour Index No	40820
Einecs	214-171-6
Chemical names	β-apo-8′-carotenal, Trans-β-apo-8′carotene-aldehyde
Chemical formula	C30H40O
Molecular weight	416,65
Assay	Not less than 96 % of total colouring matters E1 cm 1 %2 640 at ca 460-462 nm in cyclohexane
Description	Dark violet crystals with metallic lustre or crystalline powder
Identification
Spectrometry	Maximum in cyclohexane at 460-462 nm
Purity
Sulfated ash	Not more than 0,1 %
Subsidiary colouring matters	Carotenoids other than β-apo-8′-carotenal: not more than 3,0 % of total colouring matters
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 160f ETHYL ESTER OF BETA-APO-8′-CAROTENOIC ACID (C30)
Synonyms	CI Food Orange 7, β-apo-8′-carotenoic ester
Definition	These specifications apply to predominantly all trans isomer of β-apo-8′-carotenoic acid ethyl ester together with minor amounts of other carotenoids. Diluted and stabilized forms are prepared from β-apo-8′-carotenoic acid ethyl ester meeting these specifications and include solutions or suspensions of β-apo-8′-carotenoic acid ethyl ester in edible fats or oils, emulsions and water dispersible powders. These preparations may have different cis/trans isomer ratios.
Class	Carotenoid
Colour Index No	40825
Einecs	214-173-7
Chemical names	β-apo-8′-carotenoic acid ethyl ester, ethyl 8′-apo-β-caroten-8′-oate
Chemical formula	C32H44O2
Molecular weight	460,7
Assay	Not less than 96 % of total colouring matters E1 cm 1 %2 550 at ca 449 nm in cyclohexane
Description	Red to violet-red crystals or crystalline powder
Identification
Spectrometry	Maximum in cyclohexane at ca 449 nm
Purity
Sulfated ash	Not more than 0,1 %
Subsidiary colouring matters	Carotenoids other than β-apo-8′-carotenoic acid ethyl ester: not more than 3,0 % of total colouring matters
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 161b LUTEIN
Synonyms	Mixed Carotenoids, Xanthophylls
Definition	Lutein is obtained by solvent extraction of the natural strains of edible fruits and plants, grass, lucerne (alfalfa) and tagetes erecta. The main colouring principle consists of carotenoids of which lutein and its fatty acid esters account for the major part. Variable amounts of carotenes will also be present. Lutein may contain fats, oils and waxes naturally occurring in the plant material. Only the following solvents may be used for the extraction: methanol, ethanol, propan-2-ol, hexane, acetone, methyl ethyl ketone, dichloromethane and carbon dioxide
Class	Carotenoid
Einecs	204-840-0
Chemical names	3,3′-dihydroxy-d-carotene
Chemical formula	C40H56O2
Molecular weight	568,88
Assay	Content of total colouring matter not less than 4 % calculated as lutein E1 cm 1 %2 550 at ca 445 nm in chloroform/ethanol (10 + 90) or in hexane/ethanol/acetone (80 + 10 + 10)
Description	Dark, yellowish brown liquid
Identification
Spectrometry	Maximum in chloroform/ethanol (10 + 90) at ca 445 nm
Purity
Solvent residues	Acetone Methyl ethyl ketone Methanol Ethanol Propan-2-ol Hexane	Not more than 50 mg/kg, singly or in combination
	Dichloromethane: not more than 10 mg/kg
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 161g CANTHAXANTHIN
Synonyms	CI Food Orange 8
Definition	These specifications apply to predominantly all trans isomers of canthaxanthin together with minor amounts of other carotenoids. Diluted and stabilized forms are prepared from canthaxanthin meeting these specifications and include solutions or suspensions of canthaxanthin in edible fats or oils, emulsions and water dispersible powders. These preparations may have different cis/trans isomer ratios.
Class	Carotinoid
Colour Index No	40850
Einecs	208-187-2
Chemical names	β-Carotene-4,4′-dione, canthaxanthin, 4,4′-dioxo-β-carotene
Chemical formula	C40H52O2
Molecular weight	564,86
Assay	Not less than 96 % of total colouring matters (expressed as canthaxanthin) E1 cm 1 %2 200 at ca 485 nm in chloroform at 468-472 nm in cyclohexane at 464-467 nm in petroleum ether
Description	Deep violet crystals or crystalline powder
Identification
Spectrometry	Maximum in chloroform at ca 485 nm Maximum in cyclohexane at 468-472 nm Maximum in petroleum ether at 464-467 nm
Purity
Sulfated ash	Not more than 0,1 %
Subsidiary colouring matters	Carotenoids other than canthaxanthin: not more than 5,0 % of total colouring matters
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 162 BEETROOT RED, BETANIN
Synonyms	Beet Red
Definition	Beet red is obtained from the roots of natural strains of red beets (Beta vulgaris L. var. rubra) by pressing crushed beet as press juice or by aqueous extraction of shredded beet roots and subsequent enrichment in the active principle. The colour is composed of different pigments all belonging to the class betalaine. The main colouring principle consists of betacyanins (red) of which betanin accounts for 75-95 %. Minor amounts of betaxanthin (yellow) and degradation products of betalaines (light brown) may be present. Besides the colour pigments the juice or extract consists of sugars, salts, and/or proteins naturally occurring in red beets. The solution may be concentrated and some products may be refined in order to remove most of the sugars, salts and proteins.
Class	Betalaine
Einecs	231-628-5
Chemical names	(S-(R′,R′)-4-(2-(2-Carboxy-5(β-D-glucopyranosyloxy)-2,3-dihydro-6-hydroxy-1H-indol-1-yl)ethenyl)-2,3-dihydro-2,6-pyridine-dicarboxylic acid; 1-(2-(2,6-dicarboxy-1,2,3,4-tetrahydro-4-pyridylidene)ethylidene)-5-β-D-glucopyranosyloxy)-6-hydroxyindolium-2-carboxylate
Chemical formula	Betanin: C24H26N2O13
Molecular weight	550,48
Assay	Content of red colour (expressed as betanine) is not less than 0,4 % E1 cm 1 %1 120 at ca 535 nm in aqueous solution at pH 5
Description	Red or dark red liquid, paste, powder or solid
Identification
Spectrometry	Maximum in water of pH 5 at ca 535 nm
Purity
Nitrate	Not more than 2 g nitrate anion/g of red colour (as calculated from assay).
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 163 ANTHOCYANINS
Definition	Anthocyanins are obtained by extraction with sulphited water, acidified water, carbon dioxide, methanol or ethanol from the natural strains of vegetables and edible fruits. Anthocyanins contain common components of the source material, namely anthocyanine, organic acids, tannins, sugars, minerals etc., but not necessarily in the same proportions as found in the source material.
Class	Anthocyanin
Einecs	208-438-6 (cyanidin); 205-125-6 (peonidin); 208-437-0 (delphinidin); 211-403-8 (malvidin); 205-127-7 (pelargonidin)
Chemical names	3,3′,4′,5,7-Pentahydroxy-flavylium chloride (cyanidin) 3,4′,5,7-Tetrahydroxy-3′-methoxyflavylium chloride (peonidin) 3,4′,5,7-Tetrahydroxy-3′,5′-dimethoxyflavylium chloride (malvidin) 3,5,7-Trihydroxy-2-(3,4,5,trihydroxyphenyl)-1-benzopyrylium chloride (delphinidin) 3,3′4′,5,7-Pentahydroxy-5′-methoxyflavylium chloride (petunidin) 3,5,7-Trihydroxy-2-(4-hydroxyphenyl)-1-benzopyrilium chloride (pelargonidin)
Chemical formula	Cyanidin: C15H11O6Cl Peonidin: C16H13O6Cl Malvidin: C17H15O7Cl Delphinidin: C15H11O7Cl Petunidin: C16H13O7Cl Pelargonidin: C15H11O5Cl
Molecular weight	Cyanidin: 322,6 Peonidin: 336,7 Malvidin: 366,7 Delphinidin: 340,6 Petunidin: 352,7 Pelargonidin: 306,7
Assay	E1 cm 1 % 300 for the pure pigment at 515-535 nm at pH 3,0
Description	Purplish-red liquid, powder or paste, having a slight characteristic odour
Identification
Spectrometry	Maximum in methanol with 0,01 % conc. HCl Cyanidin: 535 nm Peonidin: 532 nm Malvidin: 542 nm Delphinidin: 546 nm Petunidin: 543 nm Pelargonidin: 530 nm
Purity
Solvent residues	Methanol Ethanol	Not more than 50 mg/kg, singly or in combination
Sulfur dioxide	Not more than 1 000 mg/kg per percent pigment
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 170 CALCIUM CARBONATE
Synonyms	CI Pigment White 18, Chalk
Definition	Calcium carbonate is the product obtained from ground limestone or by the precipitation of calcium ions with carbonate ions.
Class	Inorganic
Colour Index No	77220
Einecs	Calcium carbonate: 207-439-9 Limestone: 215-279-6
Chemical names	Calcium carbonate
Chemical formula	CaCO3
Molecular weight	100,1
Assay	Content not less than 98 % on the anhydrous basis
Description	White crystalline or amorphous, odourless and tasteless powder
Identification
Solubility	Practically insoluble in water and in alcohol. Dissolves with effervescence in diluted acetic acid, in diluted hydrochloric acid and in diluted nitric acid, and the resulting solutions, after boiling, give positive tests for calcium.
Purity
Loss on drying	Not more than 2,0 % (200 °C, 4 hours)
Acid-insoluble substances	Not more than 0,2 %
Magnesium and alkali salts	Not more than 1,5 %
Fluoride	Not more than 50 mg/kg
Antimony (as Sb) Copper (as Cu) Chromium (as Cr) Zinc (as Zn) Barium (as Ba)	Not more than 100 mg/kg, singly or in combination
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Cadmium	Not more than 1 mg/kg
E 171 TITANIUM DIOXIDE
Synonyms	CI Pigment White 6
Definition	Titanium dioxide consists essentially of pure anatase and/or rutile titanium dioxide which may be coated with small amounts of alumina and/or silica to improve the technological properties of the product.
Class	Inorganic
Colour Index No	77891
Einecs	236-675-5
Chemical names	Titanium dioxide
Chemical formula	TiO2
Molecular weight	79,88
Assay	Content not less than 99 % on an alumina and silica-free basis
Description	White to slightly coloured powder
Identification
Solubility	Insoluble in water and organic solvents. Dissolves slowly in hydrofluoric acid and in hot concentrated sulfuric acid.
Purity
Loss on drying	Not more than 0,5 % (105 °C, 3 hours)
Loss on ignition	Not more than 1,0 % on a volatile matter free basis (800 °C)
Aluminium oxide and/or silicon dioxide	Total not more than 2,0 %
Matter soluble in 0,5 N HCl	Not more than 0,5 % on an alumina and silica-free basis and, in addition, for products containing alumina and/or silica, not more than 1,5 % on the basis of the product as sold.
Water soluble matter	Not more than 0,5 %
Cadmium	Not more than 1 mg/kg
Antimony	Not more than 50 mg/kg by total dissolution
Arsenic	Not more than 3 mg/kg by total dissolution
Lead	Not more than 10 mg/kg by total dissolution
Mercury	Not more than 1 mg/kg by total dissolution
Zink	Not more than 50 mg/kg by total dissolution
E 172 IRON OXIDES AND IRON HYDROXIDES
Synonyms	Iron Oxide Yellow : CI Pigment Yellow 42 and 43 Iron Oxide Red : CI Pigment Red 101 and 102 Iron Oxide Black : CI Pigment Black 11
Definition	Iron oxides and iron hydroxides are produced synthetically and consist essentially of anhydrous and/or hydrated iron oxides. The range of hues includes yellows, reds, browns and blacks. Food quality iron oxides are primarily distinguished from technical grades by the comparatively low levels of contamination by other metals. This is achieved by the selection and control of the source of the iron and/or by the extent of chemical purification during the manufacturing process.
Class	Inorganic
Colour Index No	Iron Oxide Yellow : 77492 Iron Oxide Red : 77491 Iron Oxide Black : 77499
Einecs	Iron Oxide Yellow : 257-098-5 Iron Oxide Red : 215-168-2 Iron Oxide Black : 235-442-5
Chemical names	Iron Oxide Yellow : hydrated ferric oxide, hydrated iron (III) oxide Iron Oxide Red : anhydrous ferric oxide, anhydrous iron (III) oxide Iron Oxide Black : ferroso ferric oxide, iron (II, III) oxide
Chemical formula	Iron Oxide Yellow : FeO(OH)·H2O Iron Oxide Red : Fe2O3 Iron Oxide Black : FeO·Fe2O3
Molecular weight	88,85 : FeO(OH) 159,70 : Fe2O3 231,55 : FeO·Fe2O3
Assay	Yellow not less than 60 %, red and black not less than 68 % total iron, expressed as iron
Description	Powder; yellow, red, brown or black in hue
Identification
Solubility	Insoluble in water and in organic solvents Soluble in concentrated mineral acids
Purity
Water soluble matter	Not more than 1,0 %	By total dissolution
Arsenic	Not more than 5 mg/kg
Barium	Not more than 50 mg/kg
Cadmium	Not more than 5 mg/kg
Chromium	Not more than 100 mg/kg
Copper	Not more than 50 mg/kg
Lead	Not more than 20 mg/kg
Mercury	Not more than 1 mg/kg
Nickel	Not more than 200 mg/kg
Zinc	Not more than 100 mg/kg
E 173 ALUMINIUM
Synonyms	CI Pigment Metal, Al
Definition	Aluminium powder is composed of finely divided particles of aluminium. The grinding may or may not be carried out in the presence of edible vegetable oils and/or food additive quality fatty acids. It is free from admixture with substances other than edible vegetable oils and/or food additive quality fatty acids.
Colour Index No	77000
Einecs	231-072-3
Chemical names	Aluminium
Chemical formula	Al
Atomic weight	26,98
Assay	Not less than 99 % calculated as Al on an oil-free basis
Description	A silvery-grey powder or tiny sheets
Identification
Solubility	Insoluble in water and in organic solvents. Soluble in dilute hydrochloric acid. The resulting solution gives positive tests for aluminium.
Purity
Loss on drying	Not more than 0,5 % (105 °C, to constant weight)
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg
E 174 SILVER
Synonyms	Argentum, Ag
Class	Inorganic
Colour Index No	77820
Einecs	231-131-3
Chemical names	Silver
Chemical formula	Ag
Atomic weight	107,87
Assay	Content not less than 99,5 % Ag
Description	Silver-coloured powder or tiny sheets
E 175 GOLD
Synonyms	Pigment Metal 3, Aurum, Au
Class	Inorganic
Colour Index No	77480
Einecs	231-165-9
Chemical names	Gold
Chemical formula	Au
Atomic weight	197,0
Assay	Content not less than 90 % Au
Description	Gold-coloured powder or tiny sheets
Purity
Silver	Not more than 7,0 %	After complete dissolution
Copper	Not more than 4,0 %
E 180 LITHOLRUBINE BK
Synonyms	CI Pigment Red 57, Rubinpigment, Carmine 6B
Definition	Lithol Rubine BK consists essentially of calcium 3-hydroxy-4-(4-methyl-2-sulfonatophenylazo)-2-naphthalenecarboxylate and subsidiary colouring matters together with water, calcium chloride and/or calcium sulfate as the principal uncoloured components.
Class	Monoazo
Colour Index No	15850:1
Einecs	226-109-5
Chemical names	Calcium 3-hydroxy-4-(4-methyl-2-sulfonatophenylazo)-2-naphthalene-carboxylate
Chemical formula	C18H12CaN2O6S
Molecular weight	424,45
Assay	Content not less than 90 % total colouring matters E1 cm 1 % 200 at ca 442 nm in dimethylformamide
Description	Red powder
Identification
Spectrometry	Maximum in dimethylformamide at ca 442 nm
Purity
Subsidiary colouring matters	Not more than 0,5 %
Organic compounds other than colouring matters:
2-Amino-5-methylbenzenesulfonic acid, calcium salt	Not more than 0,2 %
3-hydroxy-2-naphthalenecarboxylic acid, calcium salt	Not more than 0,4 %
Unsulfonated primary aromatic amines	Not more than 0,01 % (expressed as aniline)
Ether extractable matter	From a solution of pH 7, not more than 0,2 %
Arsenic	Not more than 3 mg/kg
Lead	Not more than 10 mg/kg
Mercury	Not more than 1 mg/kg
Cadmium	Not more than 1 mg/kg
Heavy metals (as Pb)	Not more than 40 mg/kg

Textual Amendments

F1 Substituted by Commission Directive 2011/3/EU of 17 January 2011 amending Directive 2008/128/EC laying down specific purity criteria on colours for use in foodstuffs (Text with EEA relevance).

ANNEX II U.K.

PART A U.K.

Repealed Directive with list of its successive amendments

(referred to in Article 2)

Commission Directive 95/45/EC	(OJ L 226, 22.9.1995, p. 1)
Commission Directive 1999/75/EC	(OJ L 206, 5.8.1999, p. 19)
Commission Directive 2001/50/EC	(OJ L 190, 12.7.2001, p. 14)
Commission Directive 2004/47/EC	(OJ L 113, 20.4.2004, p. 24)
Commission Directive 2006/33/EC	(OJ L 82, 21.3.2006, p. 10)

PART B U.K.

List of time-limits for transposition into national law

(referred to in Article 2)

a According to Article 2(2) of Directive 95/45/EC, products put on the market or labelled before 1 July 1996 which do not comply with that Directive may, however, be marketed until stocks are exhausted.
b According to Article 3 of Directive 2004/47/EC, products on the market or labelled before 1 April 2005 which do not comply with that Directive may be marketed until stocks are exhausted.
Directive	Time-limit for transposition
95/45/EC	1 July 1996a
1999/75/EC	1 July 2000
2001/50/EC	29 June 2002
2004/47/EC	1 April 2005b
2006/33/EC	10 April 2007

ANNEX III U.K.

Correlation table

Directive 95/45/EC	This Directive
Article 1, first paragraph	Article 1
Article 1, second paragraph	—
Article 2	—
—	Article 2
Article 3	Article 3
Article 4	Article 4
Annex	Annex I
—	Annex II
—	Annex III