| Definition | Linear, neutral glucan consisting mainly of maltotriose units connected by -1,6 glycosidic bonds. It is produced by fermentation from a food-grade hydrolysed starch using a non-toxin-producing strain of Aureobasidium pullulans. After completion of the fermentation, the fungal cells are removed by microfiltration, the filtrate is heat-sterilised and pigments and other impurities are removed by adsorption and ion exchange chromatography |
| Einecs | 232-945-1 |
| Chemical formula | (C6H10O5)x |
| Assay | Not less than 90 % of glucan on the dried basis |
| Description | White to off-white odourless powder |
| Identification | |
A.Solubility | Soluble in water, practically insoluble in ethanol |
B.pH of 10 % solution | 5,0 to 7,0 |
C.Precipitation with polyethylene glycol 600 | Add 2 ml of polyethylene glycol 600 to 10 ml of a 2 % aqueous solution of pullulan. A white precipitate is formed |
D.Depoly-merisation with pullulanase | Prepare two test tubes each with 10 ml of a 10 % pullulan solution. Add 0,1 ml pullulanase solution having activity 10 units/g to one test tube, and 0,1 ml water to the other. After incubation at about 25 oC for 20 minutes, the viscosity of the pullulanase-treated solution is visibly lower than that of the untreated solution |
| Purity | |
| Loss on drying | Not more than 6 % (90 oC, pressure not more than 50 mm Hg, 6 h) |
| Mono-, di- and oligosaccharides | Not more than 10 % expressed as glucose |
| Viscosity | 100 to 180 mm2/s (10 % w/w aqueous solution at 30 oC) |
| Lead | Not more than 1 mg/kg |
| Yeast and moulds | Not more than 100 colonies per gram |
| Coliforms | Absent in 25 g |
| Salmonella | Absent in 25 g |
