Council Directive 2009/158/ECShow full title

[F1 A. Salmonella Pullorum (1) , Salmonella Gallinarum (2) and Salmonella arizonae (3) infections U.K.

1. Species concerned U.K.

(a)

Salmonella Pullorum and Salmonella Gallinarum: fowls, turkeys, guinea fowls, quails, pheasants, partridges and ducks.

(b)

Salmonella arizonae : turkeys.

2. Disease surveillance programme U.K.

(a)

Serological and/or bacteriological tests must be used to determine whether an infection is present (4) .

(b)

Samples for testing must be taken, as appropriate, from blood, embryos that fail to hatch (namely embryos dead-in-shell), second grade chicks, meconium, post mortem tissues, especially liver, spleen, ovary/oviduct and ileo-caecal junction (5) .

(c)

Direct enrichment in Selenite-cysteine broth for faecal/meconium and intestinal samples is to be used. Non-selective pre-enrichment followed by selective enrichment in soya based Rappaport-Vassiliadis (RVS) broth or Müller-Kauffmann Tetrathionate-novobiocin broth (MKTTn) may be used for samples (such as embryos dead-in-shell) where competing flora is expected to be minimal (6) , (7) .

(d)

When blood samples are taken from a flock for serological testing for Salmonella Pullorum and Salmonella Gallinarum or Salmonella arizonae , the prevalence of infection in the Member State concerned and its past incidence in the establishment must be allowed for in determining the number of samples to be taken. However, a statistically valid number of samples for serological and/or bacteriological testing to detect infection must always be taken.

(e)

Flocks must be inspected during each laying period at the best time for detecting the disease in question.

(f)

Samples for bacteriological testing must not be taken from poultry or eggs that have been treated with antimicrobial medicinal products during the 2 to 3 weeks prior to testing.

(g)

Detection techniques must be capable of differentiating serological responses to Salmonella Pullorum and Salmonella Gallinarum infection from serological responses due to the use of Salmonella Enteritidis vaccine, where this vaccine is used (8) . Such vaccination must therefore not be used if serological monitoring is to be used. If vaccination has been used, bacteriological testing must be used, but the confirmation method used must be capable of differentiating live vaccinal strains from field strains.]

Textual Amendments

F1 Substituted by Commission Decision of 1 April 2011 amending Annexes II to IV to Council Directive 2009/158/EC on animal health conditions governing intra-Community trade in, and imports from third countries of, poultry and hatching eggs (notified under document C(2011) 2068) (Text with EEA relevance) (2011/214/EU).

(1)

[F1 Salmonella Pullorum means Salmonella enterica subspecies enterica serovar Gallinarum biochemical variant (biovar) Pullorum.]

(2)

[F1 Salmonella Gallinarum means Salmonella enterica subspecies enterica serovar Gallinarum biochemical variant (biovar) Gallinarum.]

(3)

[F1 Salmonella arizonae means Salmonella enterica subspecies arizonae serogroup K (O18) arizonae.]

(4)

[F1 Note that serological testing in avian species other than fowls may sometimes result in an unacceptable proportion of false-positive reactions.]

(5)

[F1 [Note that environmental samples are generally not suitable for reliable detection of Salmonella pullorum and Salmonella gallinarum , but are suitable for Salmonella arizonae.] ]

(6)

[F1 Note that direct plating of aseptically collected tissues on to a minimally selective agar, such as MacConkey agar, is also useful for diagnosis.]

(7)

[F1 [Salmonella pullorum and Salmonella gallinarum do not readily grow in the modified semi-solid Rappaport Vassiliadis (MSRV) medium that is used for monitoring of zoonotic Salmonella spp. in the Union, but it is suitable for Salmonella arizonae.] ]

(8)

[F1 Note that there is currently no test that can differentiate between the response to Salmonella Pullorum and Salmonella Gallinarum infection and vaccination for this serotype.]

Textual Amendments

F1 Substituted by Commission Decision of 1 April 2011 amending Annexes II to IV to Council Directive 2009/158/EC on animal health conditions governing intra-Community trade in, and imports from third countries of, poultry and hatching eggs (notified under document C(2011) 2068) (Text with EEA relevance) (2011/214/EU).