- Latest available (Revised)
- Point in Time (01/01/2005)
- Original (As adopted by EU)
Commission Regulation (EC) No 796/2004 of 21 april 2004 laying down detailed rules for the implementation of cross-compliance, modulation and the integrated administration and control system provided for in of Council Regulation (EC) No 1782/2003 establishing common rules for direct support schemes under the common agricultural policy and establishing certain support schemes for farmers (repealed)
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Version Superseded: 25/03/2005
Point in time view as at 01/01/2005.
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This method seeks to determine the Δ9-tetrahydrocannabinol (hereinafter referred to as ‘THC’) content of varieties of hemp (Cannabis sativa L.) As appropriate, the method involves applying procedure A or B herein described.
The method is based on the quantitative determination of Δ9-THC by gas chromatography (GC) after extraction with a suitable solvent.
Procedure A shall be used for checks on production as provided for in Article 52(1) of Regulation (EC) No 1782/2003 and Article 26(2)(a) of this Regulation.
Procedure B shall be used in cases as referred to in Article 52(2) of Regulation (EC) No 1782/2003 and Article 33(4) of this Regulation.
Procedure A: in a standing crop of a given variety of hemp, a 30 cm part containing at least one female inflorescence of each plant selected shall be taken. Sampling shall be carried out during the period running from 20 days after the start of flowering to 10 days after the end of flowering, during the day, following a systematic pattern to ensure that the sample is representative of the field but excluding the edges of the crop.
Member States may authorise sampling to be carried out during the period from the start of flowering to 20 days after the start of flowering provided that, for each variety grown, other representative samples are taken in accordance with the above rules during the period from 20 days after the start of flowering to 10 days after the end of flowering.
Procedure B: in a standing crop of a given variety of hemp, the upper third of each plant selected shall be taken. Sampling shall be carried out during the 10 days following the end of flowering, during the day, following a systematic pattern to ensure that the sample is representative of the field but excluding the edges of the crop. In the case of dioecious varieties, only female plants shall be taken.
Procedure A: the sample shall comprise parts of 50 plants per field.
Procedure B: the sample shall comprise parts of 200 plants per field.
Each sample shall be placed in a fabric or paper bag, without crushing it, and be sent to the laboratory for analysis.
The Member State may provide for a second sample to be collected for counteranalysis, if required, to be kept either by the producer or by the body responsible for the analysis.
Drying of the samples shall begin as soon as possible and, in any case, within 48 hours using any method below 70 oC. Samples should be dried to a constant weight and to a moisture content of between 8 % and 13 %.
After drying, the samples shall be stored without crushing them at below 25 oC in a dark place.
Stems and seeds over 2 mm in size shall be removed from the dried samples.
The dried samples shall be grinded to obtain a semi-fine powder (passing through a 1 mm mesh sieve).
The powder may be stored for 10 weeks at below 25 oC in a dark, dry place.
Reagents
Δ9-tetrahydrocannabinol, pure for chromatographic purposes,
Squalane, pure for chromatographic purposes, as an internal standard.
Extraction solution
35 mg of squalane per 100 ml hexane.
100 mg of the powdered test sample shall be weighed, be placed in a centrifuge tube and 5 ml of extraction solution shall be added containing the internal standard.
Place in an ultrasound bath and leave for 20 minutes. Centrifuge for five minutes at 3000 r.p.m. and then remove the supernatant THC solution. Inject the solution into the chromatograph and carry out a quantitative analysis.
Apparatus
gas chromatograph with a flame ionisation detector and a split/splitless injector,
column allowing good separation of cannabinoids, for example a glass capillary column 25 m long and 0.22 mm in diameter impregnated with a 5 % non-polar phenyl-methyl-siloxane phase.
Calibration ranges
At least three points for procedure A and five points for procedure B, including points 0.04 and 0.50 mg/ml Δ9-THC in extraction solution.
Experimental conditions
The following conditions are given as an example for the column referred to in (a):
oven temperature 260 oC
injector temperature 300 oC
detector temperature 300 oC
Volume injected: 1 μl
The findings shall be expressed to two decimal places in grams of Δ9-THC per 100 grams of analytical sample dried to constant weight. A tolerance of 0.03 g per 100 g shall apply.
Procedure A: one determination per test sample.
However, where the result obtained is above the limit laid down in Article 52(1) of Regulation (EC) No 1782/2003, a second determination shall be carried out per analysis sample and the mean value of the two determinations shall be taken as the result.
Procedure B: the result corresponds to the mean value of two determinations per test sample.
Carmagnola
Beniko
Chamaeleon
Cs
Delta-Ilosa
Delta 405
Dioica 88
Epsilon 68
Fedora 17
Felina 32
Ferimon – Férimon
Fibranova
Fibrimon 24
Futura 75
Juso 14
Red Petiole
Santhica 23
Santhica 27
Uso 31
Bialobrzeskie
Cannacomp(1)
Fasamo
Felina 34 – Félina 34
Fibriko TC
Finola
Lipko(2)
Silesia(3)
Tiborszállási(2)
UNIKO-B
Articles of Commission Regulation (EC) No 2419/2001 | Articles of this Regulation |
---|---|
1 | – |
2 | 2 |
3 | 5 |
4(1) | 6(1) |
4(2) | 14(4) |
5 | 8 |
6 | – |
7 | – |
8 | 15 |
9 | – |
10 | 16 |
11 | 18 |
12 | 19 |
13 | 21 |
14 | 22 |
15 | 23(1) |
16 | 24 |
17(1) | 25(1) |
17(2) | 25(2) |
17(3) | 23(2) |
18 | 26 |
19 | 27 |
2 | 28 |
21 | 29 |
22 | 30 |
23 | 32 |
24 | 34 |
25 | 35 |
26 | 36 |
27 | 37 |
28 | 38 |
29 | 39 |
30 | 49 |
31(1) | 50(1) |
31(2) | 50(3) |
31(3) | 50(6) |
31(4) | 50(7) |
32 | 51 |
33 | 53 |
34 | 55 |
35 | 56 |
36 | 57 |
37 | 58 |
38 | 59 |
39 | – |
40 | 60 |
41 | 61 |
42 | 62 |
43 | 63 |
44 | 68 |
45 | 69 |
46 | 70 |
47 | 71 |
48 | 72 |
49 | 73 |
50 | 74 |
51 | 75 |
52 | 76 |
53 | – |
54 | – |
only in Hungary
only in Hungary
only in Poland
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