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Commission Regulation (EC) No 273/2008 of 5 March 2008 laying down detailed rules for the application of Council Regulation (EC) No 1255/1999 as regards methods for the analysis and quality evaluation of milk and milk products (repealed)
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DETERMINATION OF THE CONTENT OF ENANTHIC ACID TRIGLYCERIDE IN BUTTER,...
7.1. Preparation of the test sample and test portion
7.1.2.1. Bring the test sample to a temperature of 20 °C ±...
7.1.2.3. Dilute a suitable amount of test sample so as to...
7.1.2.4. Proceed as with raw milk and homogenized milk (see ISO...
7.1.2.5. Weigh in a 10 ml volumetric flask (5.2), to the nearest...
7.1.2.6. Introduce 1 ml of the solution 7.1.1.2 in a 10 ml volumetric...
7.2. Preparation of the calibration standards
7.2.1. Dissolve 100 mg of the triglyceride of enanthic acid (4.3) in...
7.2.2. Dissolve 100 mg of the triglyceride of caproic acid (4.2) in...
7.2.3. Introduce 1 ml of the solution 7.2.2 in a 10 ml volumetric...
7.2.4. Introduce 1 ml of the solution 7.2.1 and 1 ml of the solution...
7.2.5. Introduce 1 ml of the solution 7.2.4 in a 10 ml volumetric...
10. TOLERANCE LIMITS: LOWER LIMITS (CASE OF INSUFFICIENT QUANTITIES)
11. TOLERANCE LIMITS: UPPER LIMITS (CASE OF EXCEEDING QUANTITY BY MORE...
12. ADDITIONAL INFORMATION: STATISTICAL ANALYSIS OF RESULTS ON THE DETERMINATION OF...
DETERMINING THE VANILLIN CONTENT IN CONCENTRATED BUTTER, BUTTER OR CREAM...
3.1. Freezer, operating in the temperature range –15 °C to –20 °C
3.3. Membrane microfilters of 0,45 μm pore size, resistant to a...
3.4. Liquid chromatography system consisting of a pump (flow of 1,0...
3.5. Analytical column (250 mm × 4,6 mm ID) packed with LiChrospher RP...
3.6. Guard column (ca. 20 mm × 3 mm ID) dry-packed with LiChrospher...
DETERMINING THE ETHYL ESTER OF BETA-APO-8'-CAROTENIC ACID IN CONCENTRATED BUTTER...
DETERMINING SITOSTEROL OR STIGMASTEROL IN BUTTER OR CONCENTRATED BUTTER BY...
3.1. 150 ml saponification flask fitted with a reflux condenser having ground-glass...
3.4. Pressure equalising funnels, 250 ml or similar, to collect waste diethyl...
3.5. Glass column, 350 mm × 20 mm fitted with sintered glass plug...
3.8. Gas chromatograph suitable for use with a capillary column, provided...
3.9. A fused-silica capillary column entirely coated with BP1 or equivalent...
3.10. A 1 μl gas chromatography microsyringe with hardened needle.
4.2. Potassium hydroxide, 60 % solution, dissolve 600 g potassium hydroxide (minimum 85 %)...
4.4. Diethyl ether, analytical purity (free from peroxides or residue)
4.5. Sodium sulphate, anhydrous, granular, previously dried at 102 °C for two...
4.6. Silylating reagent, for example TRI-SIL (available from Pierce Chemical Co,...
4.8. Sitosterol, of known purity not less than 90 % pure (P)...
4.9. Stigmasterol, of known purity not less than 90 % pure (P)...
4.10. Resolution test mixture. Prepare a solution containing 0,05 mg/ml lanosterol...
REFERENCE METHOD FOR THE DETECTION OF COWS’ MILK AND CASEINATE...
3.1. Isolation of caseins from cheese and the reference standards
3.2. Dissolving of the isolated caseins and submitting to plasmin (EC.3.4.21.7)...
3.3. Isoelectric focusing of plasmin-treated caseins in the presence of urea...
3.4. Evaluation of stained γ3 and γ2-casein patterns (evidence of cows’...
5.1. Glass plates (265 × 125 × 4 mm); rubber roller (width...
5.3. Covering sheet (280 × 125 mm). Stick on strip of adhesive...
5.4. Electrofocusing chamber with cooling plate (e.g. 265 × 125 mm) and...
5.5. Circulation cryostat, thermostatically controlled at 12 ± 0,5 °C
5.8. Plastic dropping bottles for the anode and cathode solutions
5.9. Sample applicators (10 × 5 mm, viscose or low protein-adsorption filter...
5.11. Stainless steel or glass staining and destaining dishes (e.g. 280...
5.12. Adjustable rod homogenizer (10 mm shaft diameter), rpm range 8 000...
5.18. Thermostatically controlled water bath adjustable to 35 and 40 ± 1 °C with...
DETECTION OF RENNET WHEY IN SKIMMED-MILK POWDER FOR PUBLIC STORAGE...
6.2. Optional centrifuge capable of attaining a centrifugal force of 2...
6.6. Filter papers, medium filtration, diameter about 12,5 cm
6.7. Glass filtration equipment with 0,45 μm pore diameter membrane filter...
6.8. Graduated pipettes allowing delivery of 10 ml (ISO 648, Class A,...
6.9. Dispensing system capable of delivering 20,0 ml water at ca. 50 °C...
DETERMINING RENNET WHEY SOLIDS IN SKIMMED-MILK POWDER AND THE MIXTURES...
1. PURPOSE: DETECTING THE ADDITION OF RENNET WHEY SOLIDS TO:
6.2. Optional centrifuge capable of attaining a centrifugal force of 2...
6.6. Filter papers, medium filtration, diameter about 12,5 cm
6.7. Glass filtration equipment with 0,45 μm pore diameter membrane filter...
6.8. Graduated pipettes, allowing delivery of 10 ml (ISO 648, Class A,...
6.9. Dispensing system capable of delivering 20,0 ml water at ca. 50 °C...
SKIMMED-MILK POWDER: QUANTITATIVE DETERMINATION OF PHOSPHATIDYLSERINE AND PHOSPHATIDYLETHANOLAMINE
5.1. Analytical balance, capable of weighing to the nearest 1 mg, with...
5.5. Graduated pipettes, capable of delivering 0,2, 0,5 and 5 ml
5.13.1. Gradient pumping system, capable of operating at 1,0 ml/min at...
5.13.3. Column heater, capable of maintaining the column at 30 °C ±...
5.13.4. Fluorescence detector, capable of operating at 330 nm excitation wavelength and 440 nm...
5.13.5. Integrator or data processing software capable of peak area measurement...
QUANTITATIVE DETERMINATION OF SKIMMED-MILK POWDER IN COMPOUND FEEDINGSTUFFS BY ENZYMATIC...
DETERMINATION OF MOISTURE CONTENT IN DRIED CREAM
4.1. Analytical balance, capable of weighing to the nearest 1 mg, with...
4.2. Drying oven, well ventilated, capable of being maintained thermostatically at...
4.3. Desiccator, provided with freshly dried silica gel with hygrometric indicator...
4.4. Flat-bottomed dishes, depth of approximately 25 mm, diameter of approximately 50 mm,...
4.5. Bottles, with tight-fitting stoppers, for mixing the laboratory samples.
REFERENCE METHOD FOR THE DETERMINATION OF MILK FAT PURITY BY...
5.3. Extrelut column, of capacity 1 ml to 3 ml, filled with silica gel,...
5.4. Graphite seals, capable of withstanding temperatures of at least 400 °C;...
5.5. Water bath, capable of maintaining a temperature of 50 °C ±...
5.6. Oven, capable of operating at 50 °C ± 2 °C and 100 °C ±...
5.14. Ampoules, of nominal capacity 1 ml, fitted with a polytetrafluoroethylene-lined aluminium...
5.15. Injection syringe, with syringe plunger not reaching into the tip...
5.16. Analytical balance, capable of weighing to the nearest 1 mg, with...
8. INTEGRATION, EVALUATION AND CONTROL OF THE ANALYTICAL PERFORMANCE
9. CALCULATION AND EXPRESSION OF RESULTS
PREPARATION OF THE PACKED COLUMN
A.1.3 Cocoa butter solution, with a mass fraction of 5 % cocoa...
A.1.4 Stationary phase, 3 % OV-1 on 125 μm to 150 μm (100 to 120 mesh)...
A.1.5 Glass column, of internal diameter 2 mm and length 500 mm, U-shaped....
A.1.6 Apparatus, for filling the packed column.
A.1.6.1 Filling column, with screwed-on end caps, provided with a mark...
A.1.6.2 Fine sieve, with mesh size of about 100 μm, with screw...
A.1.6.4 Vibrator, for uniform distribution of the stationary phase during filling....
A.1.6.5 Silanizing devices, for silanizing the glass surface of the column....
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