Commission Regulation (EU) No 206/2010Show full title

[X1 ANNEX I U.K. UNGULATES

Editorial Information

X1 Substituted by Corrigendum to Commission Regulation (EU) No 206/2010 of 12 March 2010 laying down lists of third countries, territories or parts thereof authorised for the introduction into the European Union of certain animals and fresh meat and the veterinary certification requirements (Official Journal of the European Union L 73 of 20 March 2010).

PART 1 U.K. List of third countries, territories or parts thereof (1)

a Exclusively for live animals other than animals belonging to the cervidae species.
b Certificates in accordance with the Agreement between the European Community and the Swiss Confederation on trade in agricultural products ( OJ L 114, 30.4.2002, p. 132 ).
c The former Yugoslav Republic of Macedonia: the definitive nomenclature for this country will be agreed following current negotiations at UN level.
d Not including Kosovo which is at present under international administration pursuant to United Nations Security Council Resolution 1244 of 10 June 1999 .
ISO code and name of third country	Code of Territory	Description of third country, territory or part thereof	Veterinary certificate		Specific conditions
			Model(s)	SG
1	2	3	4	5	6
CA – Canada	CA-0	Whole country	POR-X		IVb IX
	CA-1	Whole country, except the Okanagan Valley region of British Columbia described as follows: From a point on the Canada/United States border 120° 15′ longitude, 49° latitude Northerly to a point 119° 35′ longitude, 50° 30′ latitude North-easterly to a point 119° longitude, 50° 45′ latitude Southerly to a point on the Canada/United States border 118° 15′longitude, 49° latitude	BOV-X, OVI-X, OVI-YRUM a	A
CH – Switzerland	CH-0	Whole country	b
CL – Chile	CL-0	Whole country	BOV-X, OVI-X, RUM
			POR-X, SUI	B
GL – Greenland	GL-0	Whole country	OVI-X, RUM		V
HR – Croatia	HR-0	Whole country	BOV-X, BOV-Y, RUM, OVI-X, OVI-Y
IS – Iceland	IS-0	Whole country	BOV-X, BOV-Y RUM, OVI-X, OVI-Y
			POR-X, POR-Y	B
ME – Montenegro	ME-0	Whole country			I
MK – The former Yugoslav Republic of Macedonia c	MK-0	Whole country			I
NZ – New Zealand	NZ-0	Whole country	BOV-X, BOV-Y, RUM, POR-X, POR-Y OVI-X, OVI-Y		III V
PM – St Pierre and Miquelon	PM-0	Whole country	BOV-X, BOV-Y, RUM, OVI-X, OVI-Y CAM
RS – Serbia d	RS-0	Whole country			I

Specific Conditions (see footnotes in each certificate): U.K.

‘ I ’

:

for transit through the territory of a third country of live animals for immediate slaughter or live bovine animals for fattening which are consigned from a Member State and destined to another Member State in lorries which have been sealed with a serially numbered seal.

The seal number must be entered on the health certificate issued in accordance with the model laid down in Annex F to Directive 64/432/EEC (2) for live bovine animals for slaughter and fattening and in accordance with Model I of Annex E to Directive 91/68/EEC (3) for ovine and caprine animals for slaughter.

In addition, the seal must be intact on arrival at the designated border inspection post of entry into the Union and the seal number recorded in the integrated computerised veterinary system of the Union (TRACES).

The certificate must be stamped at the exit point of the Union by the competent veterinary authority prior to transiting one or more third countries, with the following wording ‘ONLY FOR TRANSIT BETWEEN DIFFERENT PARTS OF THE EUROPEAN UNION VIA THE FORMER YUGOSLAV REPUBLIC OF MACEDONIA/MONTENEGRO/SERBIA (4) (5)’.

Bovine animals for fattening must be transported directly to the holding of destination designated by the competent veterinary authority of destination. Those animals must not be moved from that holding unless for immediate slaughter.

‘ II ’

:

territory recognised as having an official tuberculosis-free status for the purposes of exports to the Union of live animals certified according to the model of certificate BOV-X

‘ III ’

:

territory recognised as having an official brucellosis-free status for the purposes of exports to the Union of live animals certified according to the model of certificate BOV-X.

‘ IVa ’

:

territory recognised as having an official enzootic-bovine-leukosis (EBL) free status for the purposes of exports to the Union of live animals certified according to the model of certificate BOV –X.

[F1 ‘ IVb ’

:

recognised as having officially enzootic-bovine-leukosis (EBL)-free herds equivalent to the requirements set out in Annex D to Directive 64/432/EEC for the purposes of exports to the Union of live animals certified according to the model of certificate BOV–X.]

‘ V ’

:

territory recognised as having an official brucellosis-free status for the purposes of exports to the Union of live animals certified according to the model of certificate OVI-X.

‘ VI ’

:

Geographical constraints:

‘ VII ’

:

territory recognised as having an official tuberculosis-free status for the purposes of exports to the Union of live animals certified according to the model of certificate RUM.

‘ VIII ’

:

territory recognised as having an official brucellosis-free status for the purposes of exports to the Union of live animals certified according to the model of certificate RUM.

‘ IX ’

:

territory recognised as having an official Aujeszky's disease -free status for the purposes of exports to the Union of live animals certified according to the model of certificate POR-X.

Textual Amendments

F1 Substituted by Commission Regulation (EU) No 144/2011 of 17 February 2011 amending Regulation (EU) No 206/2010 laying down lists of third countries, territories or parts thereof authorised for the introduction into the European Union of certain animals and fresh meat and the veterinary certification requirements (Text with EEA relevance).

PART 2 U.K. Models of Veterinary Certificates

Models: U.K.

‘BOV-X’

:

Model of veterinary certificate for domestic bovine animals (including Bubalus and Bison species and their cross-breeds) intended for breeding and/or production after importation.

‘BOV-Y’

:

Model of veterinary certificate for domestic bovine animals (including Bubalus and Bison species and their cross-breeds) intended for immediate slaughter after importation.

‘OVI-X’

:

Model of veterinary certificate for domestic ovine animals ( Ovis aries ) and domestic caprine animals ( Capra hircus ) intended for breeding and/or production after importation.

‘OVI-Y’

:

Model of veterinary certificate for domestic ovine animals ( Ovis aries) and domestic caprine animals ( Capra hircus ) intended for immediate slaughter after importation.

‘POR-X’

:

Model of veterinary certificate for domestic porcine animals ( Sus scrofa ) intended for breeding and/or production after importation;

‘POR-Y’

:

Model of veterinary certificate for domestic porcine animals ( Sus scrofa ) intended for immediate slaughter after importation.

‘RUM’

:

Model of veterinary certificate for animals of the order Artiodactyla (excluding bovine animals (including Bubalus and Bison species and their cross-breeds), Ovis aries , Capra hircus , Suidae and Tayassuidae ), and of the families Rhinocerotidae and Elephantidae .

‘SUI’

:

Model of veterinary certificate for non-domestic Suidae , Tayassuidae and Tapiridae .

‘CAM’

:

Model of specific attestation for animals imported from St Pierre and Miquelon under the conditions provided for in Part 7 of Annex I.

SG (Supplementary guarantees): U.K.

‘A’

:

guarantees regarding Bluetongue and Epizootic-haemorrhagic-disease tests on animals certified according to the model of certificate BOV-X (point II.2.8 B), OVI-X (point II.2.6 D) and RUM (point II.2.6).

‘B’

:

guarantees regarding Swine-vesicular-disease and Classical-swine-fever tests on animals certified according to the model of certificate POR-X (point II.2.4 B) and SUI (point II.2.4 B).

‘C’

:

guarantees regarding Brucellosis test on animals certified according to the model of certificate POR-X (point II.2.4 C) and SUI (point II.2.4 C).

[F1 Model BOV-X] U.K.

PART 3 U.K. Addendum for transport of animals by sea (To be completed and attached to the veterinary certificate when transport to the Union frontier includes, even for part of the journey, transportation by ship.)

Declaration by the master of the ship
I, the undersigned, master of ship (name …), declare that the animals referred to in the attached veterinary certificate No … have remained on board the ship during the voyage from … in … ( exporting country ) to … in the Union and that the ship did not call at any place outside … ( exporting country ) en route to the Union other than: … ( Ports of call en route ). Moreover, during the journey, these animals have not been in contact with other animals on board of a lower health status. Done at … on …
(Port of arrival)	(Date of arrival)
(stamp)	(signature of master)
	(name in capital letters and title)

PART 4 U.K. Addendum for transport of animals by air (To be completed and attached to the veterinary certificate when transport to the Union frontier includes, even for part of the journey, transportation by air.)

Declaration by the captain of the aircraft
I, the undersigned, captain of the aircraft (name …), declare that the crate or container and the area around the crate or container containing the animals referred to in the attached veterinary certificate No … has been sprayed with insecticide before departure. Done at … on …
(Airport of departure)	(Date of departure)
(stamp)	(signature of captain)
	(name in capital letters and title)

PART 5 U.K. Conditions for the approval of assembly centres (referred to in Article 4)

In order to be approved, assembly centres must meet the following requirements:

I.

They must be supervised by an official veterinarian.

II.

They must each be situated at the centre of an area of at least 20 km in diameter in which, according to official findings, there has been no case of foot-and-mouth disease for at least a period of 30 days prior to their use as approved assembly centres.

III.

They must, before each use as approved assembly centres, be cleansed and disinfected with a disinfectant officially authorised in the exporting country as effective for the control of foot-and-mouth disease.

IV.

They must have, taking into account their animal capacity:

(a)

a facility dedicated exclusively for use as an assembly centre;

(b)

appropriate facilities, that are easy to clean and disinfect, for loading, unloading and adequate housing of a suitable standard for the animals, for watering and feeding them, and for giving them any necessary treatment;

(c)

appropriate facilities for inspection and isolation;

(d)

appropriate equipment for cleaning and disinfecting rooms and trucks;

(e)

an appropriate storage area for fodder, litter and manure;

(f)

an appropriate system for collecting and disposal of waste water;

(g)

an office for the official veterinarian.

V.

When operating, they must have sufficient veterinarians to carry out all duties set out in Part 5;

VI.

They must only admit animals that are individually identified so as to guarantee traceability. To this end, when animals are admitted the owner or the person in charge of the centre must ensure that the animals are properly identified and accompanied by health documents or certificates for the species and categories involved.

In addition, the owner or the person in charge of the assembly centre must record on a register or in a data base, and retain for at least three years the name of the owner, the origin of the animals, the dates of entry and exit, the identification number of the animals or registration number of the herd of origin and the holding of destination, and, the registration number of the carrier and the registration number of the lorry delivering or collecting animals from that assembly centre.

VII.

All animals passing through the assembly centre must fulfil the health conditions established for the introduction of the relevant category of animal into the Union.

VIII.

Animals to be introduced into the Union which pass through an assembly centre must, within six days of arrival at the assembly centre, be loaded and dispatched directly to the border of the exporting country:

(a)

without coming into contact with cloven-hoofed animals other than animals which fulfil the health conditions established for the introduction of the relevant category of animal into the Union;

(b)

segregated into consignments so that no consignment contains both animals for breeding or production and animals for immediate slaughter;

(c)

in transport vehicles or containers which have first been cleansed and disinfected with a disinfectant officially authorised in the exporting country as effective for the control of foot-and-mouth disease and which are so constructed that faeces, urine, litter or fodder cannot flow or fall out during transportation.

IX.

Where the conditions for the export of animals to the Union require that a test is carried out within a specified period before loading, that period must include any period of assembly, up to six days, from the date of arrival of the animals at the approved assembly centre.

X.

The exporting third country must designate the centres which are approved for animals for breeding and production and those centres which are approved for animals for slaughter and must notify the Commission and the competent central authorities of the Member States of the names and addresses of such premises. That information must be updated regularly.

XI.

The exporting third country shall determine the procedure for official supervision of approved assembly centres and shall ensure that such supervision is carried out.

XII.

The approved assembly centres must be regularly inspected by the competent authority of the third country in order to check that the requirements for approval set out in points I to XI continue to be fulfilled.

If those inspections show that those conditions are no longer complied with, the approval of the centre must be suspended. The approval may be restored only when the competent authority of the third country is satisfied that the centre fully complies with the conditions set out in points I to XI.

PART 6 U.K. Protocols for the standardisation of materials and testing procedures (referred to in Article 5)

Tuberculosis (TBL) U.K.

The single intradermal tuberculin test using bovine tuberculin shall be carried out according to Annex B to Directive 64/432/EEC. In the case of Suidae animals, the single intradermal tuberculin test using avian tuberculin shall be carried out according to Annex B to 64/432/EEC, except that the site of injection shall be the loose skin at the base of the ear.

[F1 Brucellosis ( Brucella abortus ) (BRL) U.K.

The serum agglutination test, complement fixation test, buffered brucella antigen test, enzyme-linked immunosorbent assays (ELISA) and fluorescence polarisation assay (FPA) shall be carried out according to Annex C to Directive 64/432/EEC.]

Brucellosis (Brucella melitensis) (BRL) U.K.

Tests shall be carried out according to Annex C to Directive 91/68/EEC.

Enzootic Bovine Leukosis (EBL) U.K.

The agar gel immuno-diffusion test and the enzyme linked immuno-absorbent assay test (ELISA) shall be carried out according to paragraphs A and C of Chapter II of Annex D to Directive 64/432/EEC.

Bluetongue (BTG) U.K.

A.

The blocking or competitive ELISA test shall be carried out according to the following protocol: U.K.

The competitive ELISA using monoclonal antibody 3-17-A3 is capable of identifying antibodies to all known serotypes of bluetongue virus (BTV).

The principle of the test is the interruption of the reaction between BTV antigen and a group-specific monoclonal antibody (3-17-A3) by the addition of test serum. Antibodies to BTV present in the test serum block the reactivity of the monoclonal antibody (Mab) and result in a reduction in the expected colour development after the addition of enzyme labelled anti-mouse antibody, and chromogen/ substrate. Sera can be tested at a single dilution of 1:5 (spot test – Appendix 1) or may be titrated (serum titration – Appendix 2) to give dilution end-point. Inhibition values higher than 50 % may be regarded as positive.

Material and Reagents: U.K.

1.

Appropriate ELISA microtitre plates.

2.

Antigen: supplied as a cell extracted concentrate, prepared as described below, and stored at either – 20 °C or – 70 °C.

3.

Blocking buffer: phosphate buffered saline (PBS) containing 0,3 % BTV negative adult bovine serum, 0,1 % (v/v) Tween-20 (supplied as polyoxyethylene sorbiton monolaurate syrup) in PBS.

4.

Monoclonal antibody: 3-17-A3 (supplied as hybridoma tissue-culture supernatant) directed against the group-specific polypeptide VP7, stored at - 20 °C or freeze-dried and diluted 1/100 with blocking buffer before use.

5.

Conjugate: rabbit anti-mouse globulin (adsorbed and eluted) conjugated to horseradish peroxidase and kept in the dark at 4 °C.

6.

Chromogen and substrate: Orthophenylene diamine (OPD-chromogen) at a final concentration of 0,4 mg/ml in sterile distilled water. Hydrogen peroxide (30 %w/v-substrate) 0,05 % v/v added immediately before use (5μl H ₂ O ₂ per 10 ml OPD). ( Handle OPD with care - wear rubber gloves - suspected mutagen ).

7.

1 Molar sulphuric acid: 26,6 ml of acid added to 473,4 ml of distilled water. ( Remember Acid must be added to water, never water to acid .)

8.

Orbital shaker.

9.

ELISA plate reader ( the test may be read visually ).

Test format U.K.

Cc: conjugate control (no serum/ no monoclonal antibody); C++: strong positive serum control; C+: weak positive serum control; C-: negative serum control; Cm: monoclonal antibody control (no serum).

APPENDIX 1:

Spot dilution (1:5) format (40 sera/plate)

	Controls		Test Sera
	1	2	3	4	5	6	7	8	9	10	11	12
A	Cc	C-	1	2	3	4	5	6	7	8	9	10
B	Cc	C-	1	2	3	4	5	6	7	8	9	10
C	C++	C++
D	C++	C++
E	C+	C+
F	C+	C+
G	Cm	Cm										40
H	Cm	Cm										40

APPENDIX 2:

Serum titration format (10 sera/plate)

	Controls		Test Sera
	1	2	3	4	5	6	7	8	9	10	11	12
A	Cc	C-	1:5									1:5
B	Cc	C-	1:10									1:10
C	C++	C++	1:20									1:20
D	C++	C++	1:40									1:40
E	C+	C+	1:80									1:80
F	C+	C+	1:160									1:160
G	Cm	Cm	1:320									1:320
H	Cm	Cm	1:640									1:640

Test protocol: U.K.

Conjugate control (Cc)

:

Wells 1A and 1B are a blank control consisting of BTV antigen and conjugate. This may be used to blank the ELISA reader.

Mab control (Cm)

:

Columns 1 and 2, rows G and H are the monoclonal antibody control and contain BTV antigen, monoclonal antibody and conjugate. These wells represent maximum colour. The mean of the optical density readings from this control represents the 0 % inhibition value.

Positive control (C++, C+)

:

Columns 1 and 2, rows C-D-E-F. These wells contain BTV antigen, BTV strong and weak positive antiserum respectively, Mab and conjugate.

Negative control (C-)

:

Wells 2A and 2B are the negative controls, which contain BTV antigen, BTV negative antiserum, Mab and conjugate.

Test sera

:

For large-scale serological surveys and rapid screening, sera may be tested at a single dilution of 1:5 (Appendix 1). Alternatively, 10 sera may be tested over a dilution range from 1:5 to 1:640 (Appendix 2). This will give some indication of the titre of antibody in the test sera.

Procedure: U.K.

1.

Dilute BTV antigen to pre-titrated concentration in PBS, sonicate briefly to disperse aggregated virus (if sonicator is not available, pipette vigorously) and add 50 μl to all wells of the ELISA plate. Tap sides of plate to disperse antigen.

2.

Incubate at 37 °C for 60 minutes on an orbital shaker. Wash plates three times by flooding and emptying the wells with non-sterile PBS and blot dry on absorbent paper.

3.

Control wells: Add 100 μl of blocking buffer to Cc wells. Add 50 ul of positive and negative control sera, at a dilution of 1:5 (10 μ l sera + 40 μl blocking buffer), to respective wells C-, C+ and C++. Add 50μl blocking buffer to Mab control wells.

Spot titration method: Add a 1:5 dilution of each test serum in blocking buffer to duplicate wells of columns 3 to 12 (10 μl sera + 40 μl blocking buffer),

or

Serum titration method: Prepare a two-fold dilution series of each test sample (1:5 to 1:640) in blocking buffer across eight wells of single columns 3 to 12.

4.

Immediately after the addition of the test sera, dilute Mab 1:100 in blocking buffer and add 50 μl to all wells of the plate except for the blank control.

5.

Incubate at 37 °C for 60 minutes on an orbital shaker. Wash three times with PBS and blot dry.

6.

Dilute rabbit anti-mouse concentrate to 1/ 5 000 in blocking buffer and add 50 μl to all wells of the plate.

7.

Incubate at 37 °C for 60 minutes on an orbital shaker. Wash three times with PBS and blot dry.

8.

Thaw the O-Phenylenediamine dihydrochloride (OPD) and immediately before use add 5 μl of 30 % hydrogen peroxide to each 10 ml of OPD. Add 50 μl to all wells of the plate. Allow colour to develop for approximately 10 minutes and stop the reaction with 1 Molar sulphuric acid (50 μl per well). Colour should develop in the Mab control wells and in those wells containing sera with no antibody to BTV.

9.

Examine and record the plates either visually or using a spectrophotometric reader.

Analysis of results: U.K.

Using the software package print out the optical density (OD) values, and the percentage inhibition (PI) for test and control sera based on the mean value recorded in the antigen control wells. The date expressed as OD and PI values are used to determine whether the test has performed within acceptable limits. The upper control limits (UCL) and lower control limits (LCL) for the Mab control (antigen plus Mab in the absence of test sera) are between OD values 0,4 and 1.4. Any plate that fails to conform to the above criteria must be rejected.

If a computer software package is not available print out the OD values using the ELISA printer. Calculate the mean OD value for the antigen control wells, which is equivalent to the 100 % value. Determine the 50 % OD value and manually calculate the positivity and negativity of each sample.

Percentage inhibition (PI) value = 100 – (OD of each test control/Mean OD of Cm) × 100.

The duplicate negative control serum wells and the duplicate blank wells must record PI values between + 25 % and – 25 %, and between + 95 % and + 105 %, respectively. Failure to be within these limits does not invalidate the plate but does suggest that background colour is developing. The strong and weak positive control sera must record PI values between + 81 % and + 100 %, and between + 51 % and + 80 %, respectively.

The diagnostic threshold for test sera is 50 % (PI 50 % or OD 50 %). Samples recording PI values >50 % are recorded negative. Samples that record PI values above and below the threshold for the duplicate wells are considered doubtful; such samples may be re-tested in the spot test and/or titration. Positive samples may also be titrated to provide an indication of the degree of positivity.

Visual reading: Positive and negative samples are easily discernible by eye; weakly positive or strong negative samples may be more difficult to interpret by eye.

Preparation of BTV ELISA antigen: U.K.

1.

Wash 40-60 roux of confluent BHK-21 cells three times with serum-free Eagle's medium and infect with bluetongue virus serotype 1 in serum-free Eagle's medium.

2.

Incubate at 37 °C and examine daily for cytopathic effect (CPE).

3.

When CPE are complete in 90 % to 100 % of the cell sheet of each roux, harvest the virus by shaking any still-attached cells from the glass.

4.

Centrifuge at 2 000 to 3 000 rpm to pellet the cells.

5.

Discard the supernatant and re-suspend the cells in approximately 30 ml of PBS containing 1 % ‘ Sarkosyl ’ and 2 ml phenylmethylsulphonyl fluoride (lysis buffer). This may cause the cells to form a gel and more lysis buffer may be added to reduce this effect. (NB: phenylmethylsulphonyl fluoride is harmful - handle with extreme caution.)

6.

Disrupt the cells for 60 seconds using an ultrasonic probe at an amplitude of 30 microns.

7.

Centrifuge at 10 000 rpm for 10 minutes.

8.

Store the supernatant at + 4 °C and re-suspend the remaining cell pellet in 10 to 20 ml of lysis buffer.

9.

Sonicate and clarify, storing the supernatant at each stage, a total of three times.

10.

Pool the supernatants and centrifuge at 24 000 rpm (100,000 g) for 120 minutes at + 4 °C over a 5 ml cushion of 40 % sucrose (w/v in PBS) using 30 ml Beckmann centrifuge tubes and an SW 28 rotor.

11.

Discard the supernatant, drain the tubes thoroughly and re-suspend the pellet in PBS by sonication. Store the antigen in aliquots at – 20 °C.

Titration of BTV ELISA antigen: U.K.

Bluetongue ELISA antigen is titrated by the indirect ELISA. Twofold dilutions of antigen are titrated against a constant dilution (1/100) monoclonal antibody 3-17-A3. The protocol is as follows:

1.

Titrate a 1:20 dilution of BTV antigen in PBS across the microtitre plate in a twofold dilution series (50 μl/well) using a multichannel pipette.

2.

Incubate for one hour at 37 °C on an orbital shaker.

3.

Wash plates three times with PBS.

4.

Add 50 μl of monoclonal antibody 3-17-A3 (diluted 1/100) to each well of the microtitre plate.

5.

Incubate for one hour at 37 °C on an orbital shaker.

6.

Wash plates three times with PBS.

7.

Add 50 μl of rabbit anti-mouse globulin conjugated to horseradish peroxidase, diluted to a pre-titrated optimal concentration, to each well of the microtitre plate.

8.

Incubate for one hour at 37 °C on an orbital shaker.

9.

Add substrate and chromogen as described previously. Stop the reaction after 10 minutes by the addition of 1 Molar sulphuric acid (50 μl/well).

In the competitive assay, the monoclonal antibody must be in excess, therefore a dilution of antigen is chosen which falls on the titration curve (not on the plateau region) which gives approximately 0,8 OD after 10 minutes.

B.

The agar gel immuno-diffusion test shall be carried out according to the following protocol: U.K.

Antigen: U.K.

Precipitating antigen is prepared in any cell culture system that supports the rapid multiplication of a reference strain of bluetongue virus. BHK or Vero cells are recommended. Antigen is present in the supernatant fluid at the end of virus growth but requires 50 to 100-fold concentration to be effective. This may be achieved by any standard protein concentration procedure; virus in the antigen may be inactivated by the addition of 0,3 % (v/v) beta-propiolactone.

Known positive control serum: U.K.

Using the international reference serum and antigen a national standard serum is produced, standardised for optimal proportion against the international reference serum, freeze-dried and used as the known control serum in each test.

Test serum U.K.

Procedure

:

1 % agarose prepared in borate or sodium barbitol buffer, pH 8,5 to 9,0, is poured into a petri dish to a minimum depth of 3,0 mm. A test pattern of seven moisture-free wells, each 5,0 mm in diameter, is cut in the agar. The pattern consists of one centre well and six wells arranged round it in a circle of radius 3 cm. The central well is filled with the standard antigen. Peripheral wells 2, 4 and 6 are filled with known positive serum, wells 1, 3 and 5 are filled with test sera. The system is incubated for up to 72 hours at room temperature in a closed humid chamber.

Interpretation

:

A test serum is positive if it forms a specific precipitin line with the antigen and forms a complete line of identity with the control serum. A test serum is negative if it does not form a specific line with the antigen and it does not bend the line of the control serum. Petri dishes must be examined against a dark background and using indirect illumination.

Epizootic haemorrhagic disease (EHD) U.K.

The agar gel immuno-diffusion test shall be carried out according to the following protocol: U.K.

Antigen: U.K.

Precipitating antigen is prepared in any cell culture system that supports the rapid multiplication of the appropriate serotype(s) of epizootic haemorrhagic disease virus. BHK or Vero cells are recommended. Antigen is present in the supernatant fluid at the end of virus growth but requires 50 to 100-fold concentration to be effective. This may be achieved by any standard protein concentration procedure; virus in the antigen may be inactivated by the addition of 0,3 % (v/v) beta-propiolactone.

Known positive control serum: U.K.

Using the international reference serum and antigen a national standard serum is produced, standardised for optimal proportion against the international reference serum, freeze-dried and used as the known control serum in each test.

Test serum U.K.

Procedure

:

1 % agarose prepared in borate or sodium barbitol buffer, pH 8,5 to 9,0, is poured into a petri dish to a minimum depth of 3,0 mm. A test pattern of seven moisture-free wells, each 5,0 mm in diameter, is cut in the agar. The pattern consists of one centre well and six wells arranged round it in a circle of radius 3 cm. The central well is filled with the standard antigen. Peripheral wells 2, 4 and 6 are filled with known positive serum, wells 1, 3 and 5 are filled with test sera. The system is incubated for up to 72 hours at room temperature in a closed humid chamber.

Interpretation

:

A test serum is positive if it forms a specific precipitin line with the antigen and forms a complete line of identity with the control serum. A test serum is negative if it does not form a specific line with the antigen and it does not bend the line of the control serum. Petri dishes must be examined against a dark background and using indirect illumination.

Infectious bovine rhinotracheitis (IBR) / infectious pustular vulvo-vaginitis (IPV) U.K.

A. The serum neutralisation test shall be carried out according to the following protocol: U.K.

Serum

:

All sera are heat-inactivated at 56 °C for 30 minutes before use.

Procedure

:

The constant virus-varying serum neutralisation test on microtitre plates employs MDBK or other susceptible cells. The Colorado, Oxford or any other reference strain of the virus is used at 100 TCID50 per 0,025 ml; inactivated undiluted serum samples are mixed with an equal volume (0,025 ml) of virus suspension. The virus/serum mixtures are incubated for 24 hours at 37 °C in the microtitre plates before the MDBK cells are added. Cells are used at a concentration which forms a complete monolayer after 24 hours.

Controls

:

(i) virus infectivity assay, (ii) serum toxicity controls, (iii) uninoculated cell culture controls, (iv) reference antisera.

Interpretation

:

The results of the neutralisation test and the titre of the virus used in the test are recorded after three to six days incubation at 37 °C. Serum titres are considered negative if there is no neutralisation at a dilution of 1/2 (undiluted serum).

B. Any other test recognised in the framework of Decision 2004/558/EC (6) . U.K.

Foot-and-mouth disease (FMD) U.K.

A. Collecting oesophageal/pharyngeal samples and testing shall be carried out according to the following protocol: U.K.

Reagents

:

Prior to sampling, transport medium is prepared. Two ml volumes are dispensed in as many containers as there are animals to be sampled. The containers used must withstand freezing over solid CO ₂ or liquid nitrogen. Samples are obtained by the use of a specially-designed sputum collector or ‘probang’. To obtain a sample the probang cup is passed through the mouth, over the dorsum of the tongue and down into the upper part of the oesophagus. Attempts are made to scrape the surface epithelium of the upper oesophagus and pharynx by movements directed laterally and dorsally. The probang is then withdrawn, if possible after the animal has swallowed. The cup must be full and contain a mixture of mucus, saliva, oesophageal fluid and cellular debris. Care must be taken to ensure that each specimen contains some visible cellular material. Very rough handling which causes bleeding must be avoided. Samples from some animals may be heavily contaminated with ruminal contents. Such samples must be discarded and the mouth of the animal flushed with water, or preferably physiological saline, before repeat sampling.

Treatmentof samples:

:

Each sample collected in the probang cup is examined for quality and 2 ml added to an equal volume of transport medium in a container which can withstand freezing. The containers are tightly closed, sealed, disinfected and labelled. The samples are kept cool (+ 4 °C) and examined within three to four hours or placed over dry ice (- 69 °C) or liquid nitrogen and kept frozen until examined. Between animals the probang is disinfected and washed in three changes of clean water.

Testing for FMD virus:

:

Samples are inoculated into cultures of primary bovine thyroid cell cultures using at least three tubes per sample. Other susceptible cells such as primary bovine or porcine kidney cells can be used but it must be kept in mind that for some strains of FMD virus they are less sensitive. The tubes are incubated at 37 °C on a roller apparatus and examined daily for 48 hours for the presence of a cytopathic effect (CPE). If negative, cultures are blind passaged onto new cultures and re-examined for 48 hours. The specificity of any CPE must be confirmed.

Recommended transport media:

1.

0,08M phosphate buffer pH 7,2 containing 0,01 % bovine serum albumin, 0,002 % phenol red and antibiotics.

2.

Tissue culture medium (such as Eagle's MEM) containing 0,04 M Hepes buffer, 0,01 % bovine serum albumin and antibiotics, pH 7,2.

3.

Antibiotics (per ml final) must be added to the transport medium such as penicillin 1 000 IU, neomycin sulphate100 IU, polymyxin B sulphate50 IU, mycostatin100 IU.

B. The virus neutralisation test shall be carried out according to the following protocol: U.K.

Reagents

:

Stock FMDV antigen is prepared in cell cultures or on cattle tongues and stored at - 70 °C or less or at - 20 °C after the addition of 50 % glycerol. This is the stock antigen. FMDV is stable under these conditions and titres vary little over a period of months.

Procedure

:

The test is carried out in flat-bottomed tissue culture grade microtitre plates using susceptible cells such as IB-RS-2, BHK-21 or calf kidney cells. Sera for the test are diluted 1/4 in serum-free cell culture medium with the addition of 100 IU/ml neomycin or other suitable antibiotics. Sera are inactivated at 56 °C for 30 minutes and 0.05 ml amounts are used to prepare a twofold series on microtitre plates using 0,05 ml diluting loops. Pre-titrated virus also diluted in serum-free culture medium and containing 100 TCID50/0.05 ml is then added to each well. Following incubation at 37 °C for one hour to allow neutralisation to take place, 0,05 ml of suspension cells containing 0,5 to 1.0 × 10 ⁶ cells per 1 ml in cell culture medium containing serum free of FMD antibody is added to each well and the plates are sealed. Plates are incubated at 37 °C. Monolayers are normally confluent within 24 hours. CPE is usually sufficiently advanced at 48 hours for a microscopic reading of the test. At this time a final microscopic reading may be made or the plates may be fixed and stained for macroscopic reading, for instance using 10 % formol-saline and 0,05 % methylene blue.

Controls

:

Controls in each test include homologous antiserum of known titre, a cell control, a serum toxicity control, a medium control, and a virus titration from which the actual amount of virus in the test is calculated.

Interpretation

:

Wells with evidence of CPE are considered to be infected and neutralisation titres are expressed as the reciprocal of the final dilution of serum present in the serum/virus mixtures at the 50 % end point estimated according to the Spearman-Karber method. (Karber, G., 1931, Archiv fuer Experimentelle Pathologie und Pharmokologie, 162, 480.). Tests are considered to be valid when the actual amount of virus used per well in the test is between 101,5 and 102,5 TCID50 and when the titre of the reference serum is within twofold of its expected titre, estimated from the mode of previous titrations. When the controls are outside these limits the tests are repeated. An end point titre of 1/11 or less is taken as negative.

C. The detection and quantification of antibody by ELISA shall be carried out according to the following protocol: U.K.

Reagents

:

Rabbit antisera to 146S antigen of seven types of foot-and-mouth disease virus (FMDV) used at a predetermined optimum concentration in carbonate/bicarbonate buffer, pH 9,6. Antigens are prepared from selected strains of virus grown on monolayers of BHK-21 cells. The unpurified supernatants are used and pretitrated according to the protocol but without serum, to give a dilution which after the addition of an equal volume of PBST (phosphate buffered saline containing 0,05 % Tween-20 and phenol red indicator) would give an optical density reading of between 1,2 and 1,5. The viruses can be used inactivated. PBST is used as a diluent. Guinea-pig antisera are prepared by inoculating guinea pigs with 146S antigen of each serotype. A predetermined optimum concentration is prepared in PBST containing 10 % normal bovine serum and 5 % normal rabbit serum. Rabbit anti-guinea-pig immunoglobulin conjugated to horseradish peroxidase is used at a predetermined optimum concentration in PBST containing 10 % normal bovine serum and 5 % normal rabbit serum. Test sera are diluted in PBST.

Procedure: U.K.

1.

ELISA plates are coated with 50 μl of rabbit antiviral sera overnight in a humidity chamber at room temperature.

2.

Fifty microlitres of a duplicate, twofold series of each test serum starting at 1/4 are prepared in U-bottomed multiwell plates (carrier plates). Fifty microlitres of a constant dose of antigen are added to each well and the mixtures are left overnight at 4 °C. The addition of the antigen reduces the starting serum dilution to 1/8.

3.

The ELISA plates are washed five times with PBST.

4.

Fifty microlitres of serum/antigen mixtures are then transferred from the carrier plates to the rabbit-serum-coated ELISA plates and incubated at 37 °C for one hour on a rotary shaker.

5.

After washing, 50 μl of guinea-pig antiserum to the antigen used in point 4 is added to each well. The plates are incubated at 37 °C for one hour a rotary shaker.

6.

The plates are washed and 50 μl of rabbit anti-guinea-pig immunoglobulin conjugated to horseradish peroxidase is added to each well. The plates are incubated at 37 °C for one hour on a rotary shaker.

7.

The plates are washed and 50 μl of orthophenylene diamine containing 0,05 % H ₂ O ₂ (30 %) w/v is added to each well.

8.

The reaction is stopped after 15 minutes with 1,25M H ₂ SO ₄ .

The plates are read spectrophotometrically at 492 nm on an ELISA reader linked to a microcomputer.

Controls

:

For each antigen used 40 wells contain no serum but contain antigen diluted in PBST. A duplicated twofold dilution series of homologous bovine reference antiserum. A duplicate twofold dilution series of negative bovine serum.

Interpretation

:

Antibody titres are expressed as the final dilution of tests serum giving 50 % of the mean OD value recorded in the virus control wells where test serum is absent. Titres in excess of 1/40 are considered positive.

References

:

Hamblin C, Barnett ITR and Hedger RS (1986) ‘ A new enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus. I. Development and method of ELISA. ’ Journal of Immunological Methods, 93, 115 to 121.11.

Aujeszky's disease (AJD) U.K.

A. The serum neutralisation test shall be carried out according to the following protocol: U.K.

Serum

:

All sera are heat-inactivated at 56 °C for 30 minutes before use.

Procedure

:

The constant virus-varying serum neutralisation test on microtitre plates employs Vero or other sensitive cell systems. Aujeszky's disease virus is used at 100 TCID50 per 0,025 ml; inactivated undiluted serum samples are mixed with an equal volume (0,025 ml) of virus suspension. The virus/serum mixtures are incubated for two hours at 37 °C in the microtitre plates before the appropriate cells are added. Cells are used at a concentration which forms a complete monolayer after 24 hours.

Controls

:

(i) virus infectivity assay, (ii) serum toxicity controls, (iii) uninoculated cell culture controls, (iv) reference antisera.

Interpretation

:

The results of the neutralisation test and the titre of the virus used in the test are recorded after three to seven days incubation at 37 °C. Serum titres less than 1/2 (undiluted serum) are considered negative.

B. Any other test recognised in the framework of Decision 2008/185/EC (7) . U.K.

Transmissible gastro-enteritis (TGE) U.K.

The serum neutralisation test shall be carried out according to the following protocol:

Serum

:

All sera are heat-inactivated at 56 °C for 30 minutes before use.

Procedure

:

The constant virus-varying serum neutralisation test on microtitre plates employs A72 (dog tumour) cells or other sensitive cell systems. TGE virus is used at 100 TCID50 per 0,025 ml; inactivated undiluted serum samples are mixed with an equal volume (0,025 ml) of virus suspension. The virus/serum mixtures are incubated for 30 to 60 minutes at 37 °C in the microtitre plates before the appropriate cells are added. Cells are used at a concentration which forms a complete monolayer after 24 hours. Each cell receives 0,1 ml of cell suspension.

Controls

:

(i) virus infectivity assay, (ii) serum toxicity controls, (iii) uninoculated cell culture controls, (iv) reference antisera.

Interpretation

:

The results of the neutralisation test and the titre of the virus used in the test are recorded after three to five days incubation at 37 °C. Serum titres less than 1/2 (final dilution) are considered negative. If undiluted serum samples are toxic to the tissue cultures, these sera may be diluted 1/2 before being used in the test. This is equivalent to 1/4 final dilution of serum. Serum titres of less than 1/4 (final dilution) are considered negative in these cases.

Swine vesicular disease (SVD) U.K.

Tests for swine vesicular disease (SVD) shall be carried out according to Decision 2000/428/EC (8) .

Classical swine fever (CSF) U.K.

Tests for classical swine fever (CSF) shall be carried out according to Decision 2002/106/EC (9) .

The performance of tests for CSF must follow the guidelines set out in the relevant chapter of the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals.

The evaluation of sensitivity and specificity of the serological test for CSF must be carried out in a national laboratory with a quality assurance scheme in place. Tests employed must be shown to recognise a range of weak and strong positive reference sera and allow detection of antibody in early phase and convalescence.

PART 7 U.K. Import and quarantine animal health conditions for animals imported into St. Pierre and Miquelon within a period of less than six months prior to introduction into the Union (referred to in Article 6)

Animal species covered U.K.

Taxon
ORDER	FAMILY	GENUS AND SPECIES
Artiodactyla	Camelidae	Camelus spp., Lama spp., Vicugna spp.

CHAPTER 1 U.K. Residence and quarantine

1. Animals imported into St. Pierre and Miquelon must reside in an authorised quarantine station for a minimum period of 60 days before being dispatched for introduction into the Union. This period may be increased due to testing requirements for individual species. In addition the animals must comply with the following requirements: U.K.

(a)

Separate consignments may enter the quarantine station. However, upon entry in the quarantine station all animals of the same species in the quarantine facility must be considered as a single group, and referred to as such. The quarantine period must commence for the whole group at the time when the last animal entered the quarantine facility.

(b)

Within the quarantine station each specific group of animals must be maintained in isolation, with no direct or indirect contact with any other animals, including those from other consignments that may be present.

Each consignment must be kept in the approved quarantine station and protected from vector insects.

(c)

If, during the period of quarantine, the isolation of a group of animals is not maintained and contact is made with other animals, the quarantine period must begin again for the same duration as initially prescribed on entry into the quarantine station.

(d)

Animals to be introduced into the Union which pass through the quarantine station must be loaded and dispatched directly to the Union:

(i)

without coming into contact with animals other than animals which fulfil the health conditions established for the introduction of the relevant category of animal into the Union;

(ii)

segregated into consignments so that no consignment can came in contact with animals not eligible for importation into the Union;

(iii)

in transport vehicles or containers which have first been cleansed and disinfected with a disinfectant officially authorised in St. Pierre and Miquelon as effective in the control of the diseases referred to in Chapter 2 and which are so constructed that faeces, urine, litter or fodder cannot flow or fall out of the vehicle or container during transportation.

2. The quarantine premises must at least meet the minimum standards laid down in Annex B to Directive 91/496/EEC (10) , and the following conditions: U.K.

(a)

they must be supervised by an official veterinarian;

(b)

they must be situated at the centre of an area of at least 20 km in diameter in which, according to official findings, for at least 30 days prior to their use as a quarantine station there has been no case of foot-and-mouth disease;

(c)

they must, before being used as a quarantine station, be cleansed and disinfected with a disinfectant officially authorised in St Pierre et Miquelon as effective in the control of the diseases referred to in Chapter 2;

(d)

they must operate, taking into account their animal capacity:

(i)

a facility dedicated exclusively for the quarantine of animals, including adequate housing to a suitable standard for the animals;

(ii)

appropriate facilities, that:

• are easy to thouroughly clean and disinfect,

• include facilities for safe loading and unloading,

• are able to fulfil all watering and feeding requirements for the animals,

• allow any necessary veterinary treatment to be easily administered;

(iii)

appropriate facilities for inspection and isolation;

(iv)

appropriate equipment for cleaning and disinfecting rooms and transport vehicles;

(v)

an appropriate storage area for fodder, litter and manure;

(vi)

an appropriate system for collecting waste water;

(vii)

an office for the official veterinarian;

(e)

when operating, they must have sufficient veterinarians to carry out all duties;

(f)

they must only admit animals that are individually identified so as to guarantee traceability. To this end, when animals are admitted the owner or the person in charge of the quarantine station must ensure that the animals are properly identified and accompanied by health certificates for the species and categories involved. In addition, the owner or the person in charge of the quarantine station must record on a register or in a data base, and retain for at least three years, the name of the owner, the origin of the animals in the consignment, the dates of entry and exit of the animals in the consignment, the identification number of the animals in the consignment and their place of destination;

(g)

the competent authority must determine the procedure for official supervision of the quarantine station and must ensure that such supervision is carried out; this supervision must include regular inspections in order to ascertain that the requirements for approval continue to be fulfilled. In case of failure and suspension, the approval may only be restored when the competent authority is satisfied that the quarantine premises are in full compliance with all the conditions set out in points (a) to (g).

CHAPTER 2 U.K. Animal health tests

1. GENERAL REQUIREMENTS U.K.

The animals must be subjected to the following tests carried out on samples of blood taken, if not specified otherwise, not earlier than 21 days from the date of commencement of the isolation period.

The laboratory tests must be carried out in an approved laboratory in the Union and all laboratory tests and their results, vaccinations and treatments must be enclosed with the health certificate.

In order to keep animal interventions to a minimum, sampling, tests and any vaccinations must be grouped as far as is possible whilst respecting the minimum time intervals required by the testing protocols set out in Part 2 of this Chapter.

2. SPECIFIC REQUIREMENTS U.K.

2.1 CAMELIDAE U.K.

2.1.1 Tuberculosis U.K.

(a)

Test to be used : comparative intradermal reaction test using Bovine purified protein derivative (PPD) and Avian PPD conforming to the standards for the manufacture of bovine and avian tuberculins as described in point 2.1.2 of Annex B of Directive 64/432/EEC.

The test must be executed in the area behind the shoulder (axillary region) following the technique described in point 2.2.4 of Annex B of Directive 64/432/EEC.

(b)

Timing : the animals must be tested within two days from the date of arrival in the quarantine station and 42 days from the date of the first test.

(c)

Interpretation of tests:

the reaction shall be considered:

• negative if the increased skin thickness is less than 2 mm.

• positive if the increased skin thickness is more than 4 mm.

• inconclusive if the increased skin thickness to the bovine PPD is between 2mm and 4 mm, or more than 4 mm but less then the reaction to the avian PPD.

(d)

Options for action following testing:

If an animal presents a positive result to the intradermal-reaction to the bovine PPD, that animal shall be excluded from the group and the other animals shall be re-tested starting at least 42 days from the date of the first positive test was administered and this shall be considered as the first test described in (b).

If more than one animal of the group presents a positive result, the whole group shall be rejected for exportation to the Union.

If one or more animals of the same group present an inconclusive reaction, the whole group shall be re-tested starting at least 42 days from the date of the first test was administered and it shall be considered as the first test described in (b).

2.1.2 Brucellosis U.K.

(a)

Test to be used:

(i)

Brucella abortus : Rose Bengal test (RBT) and Serum agglutination test (SAT) as described respectively in points 2.5 and 2.6 of Annex C to Directive 64/432/EEC. In the case of a positive result, a complement-fixation test shall be performed for confirmation as described in Part 6 of Annex I to Regulation (EU) No 206/2010.

(ii)

Brucella melitensis : RBT and SAT as described respectively in points 2.5 and 2.6 of Annex C to Directive 64/432/EEC. In the case of a positive result, a complement-fixation test following the method described in Annex C to Directive 91/68/EEC shall be performed for confirmation.

(iii)

Brucella ovis : Complement fixation test as described in Annex D to Directive 91/68/EEC

(b)

Timing : the animals have to be tested within two days from the date of their arrival in the quarantine station and 42 days from the date of the first test.

(c)

Interpretation of tests:

A positive reaction to the tests shall be as defined in Annex C to Directive 64/432/EEC.

(d)

Options for action following testing:

Animals tested positive to one of the tests shall be excluded from the group and the other animals shall be re-tested starting at least 42 days from the date the first positive test was performed: this shall be considered as the first test described in (b).

Only the animals that tested negative to two consecutive tests performed as described in (b) shall be allowed for the introduction to the Union.

2.1.3 Bluetongue and Epizootic haemorrhagic disease (EHD) U.K.

(a)

Test to be used: agar gel immunodiffusion (AGID) test as described in Part 6 of Annex I to Regulation (EU) No 206/2010.

In case of a positive reaction the animals shall be tested with competitive ELISA test as described in Part 6 of Annex I to Regulation (EU) No 206/2010 to discriminate between the two diseases.

(b)

Timing:

The animals must be tested with negative result to two tests: the first within two days from the date of their arrival in the quarantine station and the second at least 21 days from date of the first test.

(c)

Options for action following testing:

(i)

Bluetongue

If one or more animals tested positive to the ELISA as described in Part 6 of Annex I to Regulation (EU) No 206/2010, the positive animal/animals shall be excluded from the group, and all the remaining animals in the group must be quarantined for 100 days starting from the date on which the samples for the positive test were collected. The group shall only be considered free of the bluetongue disease if regular checks carried out by official veterinarians throughout the duration of the quarantine period fail to reveal clinical symptoms of disease, and the quarantine station remains free of bluetongue vectors ( Culicoides ).

If a further animal presents clinical symptoms of bluetongue disease during the quarantine period as described in the first subparagraph, all the animals in the group shall be rejected for introduction into the Union.

(ii)

Epizootic haemorrhagic disease (EHD)

If one or more animals tested positive reveal the presence of antibodies to the EHD virus during confirmatory ELISA testing, the animal(s) shall be considered positive and shall be excluded from the group, and the whole group shall be subject to repeat testing beginning at least 21 days from the date of the initial positive diagnosis and again at least 21 days from the date of the repeat test, both with negative results.

If any additional animals are tested positive during either or both of the two tests carried out for repeat testing, the whole group of animals shall be rejected for introduction into the Union.

2.1.4 Foot-and-Mouth Disease (FMD) U.K.

(a)

Test to be used : Diagnostic tests (probang and serology) using ELISA and (Virus Neutralisation) (VN) techniques in accordance with the Protocols described in Part 6 of Annex I to Regulation (EU) No 206/2010.

(b)

Timing : the animals shall be tested with negative results to two tests: the first within two days from the date of their arrival in the quarantine station and the second at least 42 days from the date of the first test.

(c)

Options for action following testing : If any animal tests positive for the FMD virus, then none of the animals present in the quarantine station shall be considered as eligible for introduction into the Union.

Note : Any detection of antibodies to structural or not structural proteins of FMD virus shall be considered as a result of previous infection of FMD irrespective of the vaccination status.

2.1.5 Rinderpest U.K.

(a)

Test to be used : The competitive ELISA test as described in the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, latest version, is the prescribed test for international trade and is test of choice. Serum neutralisation test, or other recognised tests in accordance with the protocols described in relevant sections of the OIE manual may also be used.

(b)

Timing : the animals shall be tested twice: the first within two days from the date of their arrival in the quarantine station and the second at least 42 days from the date of the first test.

(c)

Options for action following testing : If any animal tests positive for the Rinderpest virus, then none of the animals present in the quarantine station shall be considered as eligible for introduction into the Union.

2.1.6 Vesicular stomatitis U.K.

(a)

Test to be used : ELISA, virus neutralisation test, or other recognised test in accordance with the protocols described in the relevant sections of the OIE manual.

(b)

Timing : the animals shall be tested twice: the first within two days from the date of their arrival in the quarantine station and the second at least 42 days from the date of the first test.

(c)

Options for action following testing : If any animal tests positive for vesicular stomatitis virus, then none of the animals present in the quarantine station shall be considered as eligible for introduction into the Union.

2.1.7 Rift valley fever U.K.

(a)

Test to be used : ELISA, virus neutralisation test, or other recognised test in accordance with the protocols described in relevant sections of the OIE manual.

(b)

Timing : the animals shall be tested twice: the first within two days from the date of their arrival in the quarantine station and the second at least 42 days from the date of the first test.

(c)

Options for action following testing : If any animal displays evidence of exposure to rift valley fever agent, then none of the animals present in the quarantine station shall be considered as eligible for introduction into the Union.

2.1.8 Lumpy skin disease U.K.

(a)

Test to be used : Serology using ELISA, virus neutralisation test, or other recognised test in accordance with the protocols described in relevant sections of the OIE manual.

(b)

Timing : the animals shall be tested twice: the first within two days from the date of their arrival in the quarantine station and the second at least 42 days from the date of the first test.

(c)

Options for action following testing : If any animal displays evidence of exposure to lumpy skin disease, then none of the animals present in the quarantine station shall be considered as eligible for introduction into the Union.

2.1.9 Crimean congo haemorrhagic fever U.K.

(a)

Test to be used : ELISA, virus neutralisation test, Immunofluorescence test or other recognised test.

(b)

Timing : the animals shall be tested twice: the first within two days from the date of their arrival in the quarantine station and the second at least 42 days from the date of the first test.

(c)

Options for action following testing : If any animal displays evidence of exposure to crimean congo haemorrhagic fever agent, then none of the animals present in the quarantine station shall be considered as eligible for introduction into the Union.

2.1.10 Surra (Trypanosoma evansi (T. evansi)) U.K.

(a)

Test to be used : The parasitic agent can be identified in concentrated blood samples in accordance with the protocols described in relevant sections of the OIE manual.

(b)

Timing : the animals shall be tested twice: the first within two days from the date of their arrival in the quarantine station and the second at least 42 days from the date of the first test.

(c)

Options for action following testing : If T. evansi is detected in any animal in the consignment, then that animal shall be considered not eligible for introduction into the Union. The remaining animals of the group shall then undergo internal and external antiparasitic treatment using suitable agents that are effective against T. evansi .

2.1.11 Malignant catarrhal fever U.K.

(a)

Test to be used : Detection of viral DNA based on identification by immunofluorescence or immunocytochemistry using the protocols described in relevant sections of the OIE manual.

(b)

Timing : the animals shall be tested twice: the first within two days from the date of their arrival in the quarantine station and the second at least 42 days from the date of the first test.

(c)

Options for action following testing : If any animal displays evidence of exposure to MCF, then none of the animals present in the quarantine station shall be considered as eligible for introduction into the Union.

2.1.12 Rabies U.K.

Vaccination : Rabies vaccination may be carried out when requested by the Member State of destination and the animal shall be blood sampled and a serum neutralisation test for antibodies carried out.

2.1.13 Enzootic bovine leucosis . (only in the case where the animals are destined for an officially enzootic-bovine-leucosis free Member State or region, as referred to in Article 2(2)(k) of Directive 64/432/EEC) U.K.

(a)

Test to be used : AGID or blocking ELISA, in accordance with the protocols described in the OIE manual, latest version.

(b)

Timing : the animals shall be tested twice: the first within two days from the date of their arrival in the quarantine station and the second at least 42 days from the date of the first test.

(c)

Options for action following testing : animals tested positive to the test described in (a) shall be excluded from the group of animals in the quarantine facility and the other animals shall be re-tested starting at least 21 days from the date of the first positive test was performed: this shall be considered as the first test described in (b).

Only the animals that tested negative to two consecutive tests performed as described in (b) shall be considered eligible for introduction into the Union.

ANNEX II U.K. FRESH MEAT

[F1 PART 1 U.K.

List of third countries, territories and parts thereof ^a

Footnotes:
a Without prejudice to specific certification requirements provided for in Union agreements with third countries.
b Meat from animals slaughtered on or before the date set out in column 7 may be imported into the Union for 90 days from that date. Consignments carried on vessels on the high seas may be imported into the Union if certified before the date set out in column 7 for 40 days from that date.(N.B.: no date in column 7 means that there are no time restrictions).
c Only meat from animals slaughtered on or after the date set out in column 8 may be imported into the Union (no date in column 8 means that there are no time restrictions).
d The former Yugoslav Republic of Macedonia; provisional code that does not prejudge in any way the definitive nomenclature for this country, which will be agreed following the conclusion of negotiations currently taking place on this subject in the United Nations.
e Not including Kosovo which is at present under international administration pursuant to United Nations Security Council Resolution 1244 of 10 June 1999
* = Requirements as in accordance with the Agreement between the European Community and the Swiss Confederation on trade in agricultural products ( OJ L 114, 30.4.2002, p. 132 ). — = No certificates are laid down and fresh meat imports are prohibited (except for those species where indicated in the line comprising the entry for the whole country).
‘ 1 ’ Category restrictions:
No offal is authorised for introduction into the Union (except, in the case of bovine species, diaphragm and masseter muscles).]
ISO code and name of third country	Code of Territory	Description of third country, territory or part thereof	Veterinary certificate		Specific conditions	Closing date b	Opening date c
			Model(s)	SG
1	2	3	4	5	6	7	8
AL – Albania	AL-0	Whole country	—
AR – Argentina	AR-0	Whole country	EQU
	AR-1	The Provinces of: Buenos Aires, Catamarca, Corrientes (except the departments of Berón de Astrada, Capital, Empedrado, General Paz, Itati, Mbucuruyá, San Cosme and San Luís del Palmar) Entre Ríos, La Rioja, Mendoza, Misiones, Part of Neuquén (excluding territory included in AR-4), Part of Río Negro (excluding territory included in AR-4), San Juan, San Luis, Santa Fe, Tucuman, Cordoba, La Pampa, Santiago del Estero, Chaco, Formosa, Jujuy and Salta, excluding the buffer area of 25 Km from the border with Bolivia and Paraguay that extends from the Santa Catalina District in the Province of Jujuy, to the Laishi District in the Province of Formosa	BOV	A	1		18 March 2005
			RUF	A	1		1 December 2007
			RUW	A	1		1 August 2010
	AR-2	Chubut, Santa Cruz and Tierra del Fuego	BOV, OVI, RUW, RUF				1 March 2002
	AR-3	Corrientes: the departments of Berón de Astrada, Capital, Empedrado, General Paz, Itati, Mbucuruyá, San Cosme and San Luís del Palmar	BOV RUF	A	1		1 December 2007
	AR-4	Part of Río Negro (except: in Avellaneda the zone located north of the Provincial road 7 and east of the Provincial road 250, in Conesa the zone located east of the Provincial road 2, in El Cuy the zone located north of the Provincial road 7 from its intersection with the Provincial road 66 to the border with the Department of Avellaneda, and in San Antonio the zone located east of the Provincial roads 250 and 2) Part of Neuquén (except in Confluencia the zone located east of the Provincial road 17, and in Picun Leufú the zone located east of the Provincial road 17)	BOV, OVI, RUW, RUF				1 August 2008
AU – Australia	AU-0	Whole country	BOV, OVI, POR, EQU, RUF, RUW, SUF, SUW
BA – Bosnia and Herzegovina	BA-0	Whole country	—
BH – Bahrain	BH-0	Whole country	—
BR – Brazil	BR-0	Whole country	EQU
	BR-1	State of Minas Gerais State of Espírito Santo; State of Goiás; State of Mato Grosso State of Rio Grande Do Sul, State of Mato Grosso Do Sul (except for the designated high surveillance zone of 15 Km from the external borders in the municipalities of Porto Murtinho, Caracol, Bela Vista, Antônio João, Ponta Porã, Aral Moreira, Coronel Sapucaia, Paranhos, Sete Quedas, Japorã, and Mundo Novo and the designated high surveillance zone in the municipalities of Corumbá and Ladário).	BOV	A and H	1		1 December 2008
	BR-2	State of Santa Catarina	BOV	A and H	1		31 January 2008
	BR-3	States of Paraná and São Paulo	BOV	A and H	1		1 August 2008
[F2 BW – Botswana	BW-0	Whole country	EQU, EQW
	BW-1	The veterinary disease control zones 3c, 4b, 5, 6, 8, 9 and 18	BOV, OVI, RUF, RUW	F	1	11 May 2011	1 December 2007
	BW-2	The veterinary disease control zones 10, 11, 13 and 14	BOV, OVI, RUF, RUW	F	1		7 March 2002
	BW-3	The veterinary disease control zone 12	BOV, OVI, RUF, RUW	F	1	20 October 2008	20 January 2009
	BW-4	The veterinary disease control zone 4a, except the intensive surveillance buffer zone of 10 km along the boundary with the foot-and-mouth disease vaccination zone and wildlife management areas	BOV	F	1		18 February 2011]
BY – Belarus	BY-0	Whole country	—
BZ – Belize	BZ-0	Whole country	BOV, EQU
CA – Canada	CA-0	Whole country	BOV, OVI, POR, EQU, SUF, SUW, RUF, RUW	G
CH – Switzerland	CH-0	Whole country	*
CL – Chile	CL-0	Whole country	BOV, OVI, POR, EQU, RUF, RUW, SUF
CN – China	CN-0	Whole country	—
CO – Colombia	CO-0	Whole country	EQU
CR – Costa Rica	CR-0	Whole country	BOV, EQU
CU – Cuba	CU-0	Whole country	BOV, EQU
DZ – Algeria	DZ-0	Whole country	—
ET – Ethiopia	ET-0	Whole country	—
FK – Falkland Islands	FK-0	Whole country	BOV, OVI, EQU
GL – Greenland	GL-0	Whole country	BOV, OVI, EQU, RUF, RUW
GT – Guatemala	GT-0	Whole country	BOV, EQU
HK – Hong Kong	HK-0	Whole country	—
HN – Honduras	HN-0	Whole country	BOV, EQU
HR – Croatia	HR-0	Whole country	BOV, OVI, EQU, RUF, RUW
IL – Israel	IL-0	Whole country	—
IN – India	IN-0	Whole country	—
IS – Iceland	IS-0	Whole country	BOV, OVI, EQU, RUF, RUW
KE – Kenya	KE-0	Whole country	—
MA – Morocco	MA-0	Whole country	EQU
ME – Montenegro	ME-0	Whole country	BOV, OVI, EQU
MG – Madagascar	MG-0	Whole country	—
MK – Former Yugoslav Republic of Macedonia d	MK-0	Whole country	OVI, EQU
MU – Mauritius	MU-0	Whole country	—
MX – Mexico	MX-0	Whole country	BOV, EQU
NA – Namibia	NA-0	Whole country	EQU, EQW
	NA-1	South of the cordon fences which extend from Palgrave Point in the west to Gam in the east	BOV, OVI,RUF, RUW	F and J	1
NC – New Caledonia	NC-0	Whole country	BOV, RUF, RUW
NI – Nicaragua	NI-0	Whole country	—
NZ – New Zealand	NZ-0	Whole country	BOV, OVI, POR, EQU, RUF, RUW, SUF, SUW
PA – Panama	PA-0	Whole country	BOV, EQU
PY – Paraguay	PY-0	Whole country	EQU
	PY-1	Whole country except for the designated high surveillance zone of 15 Km from the external borders	BOV	A	1		1 August 2008
RS – Serbia e	RS-0	Whole country	BOV, OVI, EQU
RU – Russia	RU-0	Whole country	—
	RU-1	Region of Murmansk, Yamolo-Nenets autonomous area	RUF
SV – El Salvador	SV-0	Whole country	—
SZ – Swaziland	SZ-0	Whole country	EQU, EQW
	SZ-1	Area west of the ‘ red line ’ fences which extends northwards from the river Usutu to the frontier with South Africa west of Nkalashane,	BOV, RUF, RUW	F	1
	SZ-2	The veterinary foot and mouth disease surveillance and vaccination control areas as gazetted as a Statutory Instrument under legal notice number 51 of 2001	BOV, RUF, RUW	F	1		4 August 2003
TH – Thailand	TH-0	Whole country	—
TN – Tunisia	TN-0	Whole country	—
TR – Turkey	TR-0	Whole country	—
	TR-1	The provinces of Amasya, Ankara, Aydin, Balikesir, Bursa, Cankiri, Corum, Denizli, Izmir, Kastamonu, Kutahya, Manisa, Usak, Yozgat and Kirikkale	EQU
UA – Ukraine	UA-0	Whole country	—
US – United States	US-0	Whole country	BOV, OVI, POR, EQU,SUF, SUW, RUF, RUW	G
UY – Uruguay	UY-0	Whole country	EQU
			BOV,	A	1		1 November 2001
			OVI	A	1
[F3 ZA – South Africa	ZA-0	Whole country	EQU, EQW
	ZA-1	The whole country except: the part of the foot-and-mouth disease control area situated in the veterinary regions of Mpumalanga and Northern provinces, in the district of Ingwavuma of the veterinary region of Natal and in the border area with Botswana east of longitude 28°, and the district of Camperdown, in the province of KwaZulu-Natal.	BOV, OVI, RUF, RUW	F	1	11 February 2011	]
ZW – Zimbabwe	ZW-0	Whole country	—

Textual Amendments

F2 Substituted by Commission Implementing Regulation (EU) No 801/2011 of 9 August 2011 amending Regulation (EU) No 206/2010 laying down lists of third countries, territories or parts thereof authorised for the introduction into the European Union of certain animals and fresh meat and the veterinary certification requirements (Text with EEA relevance).

F3 Substituted by Commission Implementing Regulation (EU) No 342/2011 of 8 April 2011 amending Annex II to Regulation (EU) No 206/2010 laying down lists of third countries, territories or parts thereof authorised for the introduction into the European Union of certain animals and fresh meat and the veterinary certification requirements (Text with EEA relevance).

[F4 PART 2 U.K. Models of veterinary certificates

Model(s): U.K.

‘ BOV ’

:

Model of veterinary certificate for fresh meat, including minced meat, of domestic bovine animals (including Bison and Bubalus species and their cross-breeds).

‘ OVI ’

:

Model of veterinary certificate for fresh meat, including minced meat, of domestic ovine animals ( Ovis aries ) and domestic caprine animals ( Capra hircus ).

‘ POR ’

:

Model of veterinary certificate for fresh meat, including minced meat, of domestic porcine animals ( Sus scrofa ).

‘ EQU ’

:

Model of veterinary certificate for fresh meat, excluding minced meat, of domestic solipeds ( Equus caballus , Equus asinus and their cross-breeds).

‘ RUF ’

:

Model of veterinary certificate for fresh meat, excluding offal and minced meat, of farmed non-domestic animals of the order Artiodactyla (excluding bovine animals (including Bison and Bubalus species and their cross-breeds), Ovis aries , Capra hircus , Suidae and Tayassuidae), and of the families Rhinocerotidae and Elephantidae.

‘ RUW ’

:

Model of veterinary certificate for fresh meat, excluding offal and minced meat, of wild non-domestic animals of the order Artiodactyla (excluding bovine animals (including Bison and Bubalus species and their cross-breeds), Ovis aries, Capra hircus , Suidae and Tayassuidae), and of the families Rhinocerotidae and Elephantidae.

‘ SUF ’

:

Model of veterinary certificate for fresh meat, excluding offal and minced meat, of farmed non-domestic animals belonging to the Suidae, Tayassuidae, or Tapiridae families.

‘ SUW ’

:

Model of veterinary certificate for fresh meat, excluding offal and minced meat, of wild non-domestic animals belonging to the Suidae, Tayassuidae, or Tapiridae families.

‘ EQW ’

:

Model of veterinary certificate for fresh meat, excluding offal and minced meat, of wild solipeds belonging to the subgenus Hippotigris (zebra).

SG (Supplementary guarantees) U.K.

‘ A ’

:

guarantees regarding the maturation, pH measurement and boning of fresh meat, excluding offal, certified according to the models of veterinary certificates BOV (point II.2.6), OVI (point II.2.6), RUF (point II.2.7) and RUW (point II.2.4).

‘ C ’

:

guarantees regarding the laboratory test for classical-swine-fever in the carcases from which fresh meat was obtained, certified according to the model of veterinary certificate SUW (point II.2.3 B).

‘ D ’

:

guarantees regarding swill feed on holding(s) of animals from which fresh meat certified was obtained according to the model of veterinary certificate POR (point II.2.3 d).

‘ E ’

:

guarantees regarding tuberculosis test in the animals from where fresh meat certified was obtained, according to the model of veterinary certificate BOV (point II.2.4 d).

‘ F ’

:

guarantees regarding the maturation and de-boning of fresh meat, excluding offal, certified according to the models of veterinary certificates BOV (point II.2.6), OVI (point II.2.6), RUF (point II.2.6) and RUW (point II.2.7).

‘ G ’

:

guarantees regarding 1, exclusion of offals and spinal cord; and 2, testing and origin of cervid animals in relation to chronic wasting disease as referred to in the models of veterinary certificates RUF (point II.1.7) and RUW (point II.1.8).

‘ H ’

:

supplementary guarantees required for Brazil. Concerning vaccination programmes, as the State of Santa Catarina in Brazil does not vaccinate against foot and mouth disease, the reference to a vaccination programme is not applicable for meat coming from animals originating and slaughtered in that State.

‘ J ’

:

guarantees regarding the movement of bovine, ovine and caprine animals from holdings to the slaughterhouse, which allow them to pass via an assembly centre (including markets) before being transported directly to slaughter.]

[F4 Model BOV] U.K.

[F4 Model OVI] U.K.

Textual Amendments

F4 Substituted by Commission Regulation (EU) No 810/2010 of 15 September 2010 amending Regulation (EU) No 206/2010 laying down lists of third countries, territories or parts thereof authorised for the introduction into the European Union of certain animals and fresh meat and the veterinary certification requirements (Text with EEA relevance).

ANNEX III U.K.

ANNEX IV U.K. ANIMALS REFERRED TO IN ARTICLE 1(1)(b)

PART 1 U.K. Lists of third countries, territories or parts thereof

SECTION 1 U.K. Parts of third countries or territories referred to in Article 7(2)

a Suspended from 5 May 2010 .]
[F4 Country/territory	Code of part of the country/territory	Description of part of the country/territory
US – United States	US-A	The State of Hawaii a

PART 2 U.K. Tables of animals and the corresponding model veterinary certificates

Table 1
‘QUE’ : Model of veterinary certificate for consignments of queen bees and queen bumble bees ( Apis mellifera and Bombus spp.), ‘BEE’ : Model of veterinary certificate for consignments of colonies of bumble bees ( Bombus spp.)
Order	Family	Genera/species
Hymenoptera	Apidae	Apis mellifera, Bombus spp.

ANNEX V U.K. Explanatory notes for completing the veterinary certificates (referred to in Article 18)

(a)

Veterinary certificates shall be issued by the exporting third country, based on the models set out in Part 2 of Annexes I, II and IV and Annex III according to the layout of the model that corresponds to the live animals/fresh meat concerned.

They shall contain, in the numbered order that appears in the model, the attestations that are required for any third country and, as the case may be, those supplementary guarantees that are required for the exporting third country or part thereof.

If the Member State of destination imposes, for the live animals/fresh meat concerned, additional certification requirements, attestations to certify that those requirements are fulfilled shall also be incorporated in the original form of the veterinary certificate.

(b)

Where the model certificate states that certain statements shall be kept as appropriate, statements which are not relevant, may be crossed out and initialled and stamped by the certifying officer, or completely deleted from the certificate.

(c)

A separate and unique certificate must be provided for the live animals/fresh meat that are exported from a territory or territories of the same exporting country appearing in columns 2 and 3 of Part 1 of Annex I, II or IV which are consigned to the same destination and transported in the same railway wagon, lorry, aircraft or ship.

(d)

The original of each certificate shall consist of a single sheet of paper, or, where more text is required it must be in such a form that all sheets of paper required are part of an integrated whole and indivisible.

(e)

The veterinary certificate shall be drawn up in at least one of the official languages of the Member State of the border inspection post of introduction of the consignment into the Union and of the Member State of destination. However, those Member States may authorise the certificate to be drawn up in the official language of another Member State, and accompanied, if necessary, by an official translation.

(f)

If for reasons of identification of the items of the consignment (schedule in point I.28 of the model veterinary certificate), additional sheets of paper are attached to the certificate, those sheets of paper shall also be considered as forming part of the original of the certificate by the application of the signature and stamp of the certifying officer, on each of the pages.

(g)

When the certificate, including additional schedules referred to in (f), comprises more than one page, each page shall be numbered, (page number) of (total number of pages), at the end of the page and shall bear the certificate reference number that has been designated by the competent authority at the top of the pages.

(h)

The original of the certificate must be completed and signed by an official veterinarian or by another designated official inspector where this is provided for in the model veterinary certificate. In the case of live animals, the certificate must be completed and signed within 24 hours prior to loading of the consignment for introduction into the Union. The competent authorities of the exporting third country shall ensure that rules of certification equivalent to those laid down in Directive 96/93/EC (11) are followed.

The colour of the signature shall be different from that of the printing. This requirement also applies to stamps other than those embossed or watermarked.

(i)

The certificate reference number referred to in boxes I.2 and II.a. must be issued by the competent authority.]

(1)

[X1 Without prejudice to specific certification requirements provided for by any relevant agreement between the Union and third countries.]

(2)

[X1 OJ 121, 29.7.1964, p. 1977/64 .]

(3)

[X1 OJ L 46, 19.2.1991, p. 19 .]

(4)

[X1 Delete country as applicable.]

(5)

[X1 Serbia does not include Kosovo which is at present under international administration pursuant to United Nations Security Council Resolution 1244 of 10 June 1999 .]

(6)

[X1 OJ L 249, 23.7.2004, p. 20 .]

(7)

[X1 OJ L 59, 4.3.2008, p. 19 .]

(8)

[X1 OJ L 167, 7.7.2000, p. 22 .]

(9)

[X1 OJ L 39, 9.2.2002, p. 71 .]

(10)

[X1 OJ L 268, 24.9.1991, p. 56 .]

(11)

[X1 OJ L 13, 16.1.1997, p. 28 .]

Editorial Information

X1 Substituted by Corrigendum to Commission Regulation (EU) No 206/2010 of 12 March 2010 laying down lists of third countries, territories or parts thereof authorised for the introduction into the European Union of certain animals and fresh meat and the veterinary certification requirements (Official Journal of the European Union L 73 of 20 March 2010).