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Commission Regulation (EU) No 231/2012Show full title
Commission Regulation (EU) No 231/2012 of 9 March 2012 laying down specifications for food additives listed in Annexes II and III to Regulation (EC) No 1333/2008 of the European Parliament and of the Council (Text with EEA relevance)
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- 2012 No. 231
- ANNEX
- E 392 EXTRACTS OF...
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E 392 EXTRACTS OF ROSEMARY
| Synonyms | Extract of rosemary leaf (antioxidant) |
| Definition | Extracts of rosemary contain several components, which have been proven to exert antioxidative functions. These components belong mainly to the classes of phenolic acids, flavonoids, diterpenoids. Besides the antioxidant compounds, the extracts can also contain triterpenes and organic solvent extractable material specifically defined in the following specification. |
| Einecs | 283-291-9 |
| Chemical name | Rosemary extract (Rosmarinus officinalis) |
| Description | Rosemary leaf extract antioxidant is prepared by extraction of the leaves of Rosmarinus officinalis using a food approved solvent system. Extracts may then be deodorised and decolourised. Extracts may be standardised. |
| Identification | |
|---|---|
| Reference antioxidative compounds: phenolic diterpenes | Carnosic acid (C20H28O4) and Carnosol (C20H26O4) (which comprise not less than 90 % of the total phenolic diterpenes) |
| Reference key volatiles | Borneol, Bornyl Acetate, Camphor, 1,8-Cineol, Verbenone |
| Density | > 0,25 g/ml |
| Solubility | Insoluble in water |
| Purity | |
| Loss of drying | < 5 % |
| Arsenic | Not more than 3 mg/kg |
| Lead | Not more than 2 mg/kg |
1 — Extracts of rosemary produced from dried rosemary leaves by acetone extraction.
| Description | Extracts of rosemary are produced from dried rosemary leaves by acetone extraction, filtration, purification and solvent evaporation, followed by drying and sieving to obtain a fine powder or a liquid. |
| Identification | |
|---|---|
| Content of reference antioxidative compounds | ≥ 10 % w/w, expressed as the total of carnosic acid and carnosol |
| Antioxidant/Volatiles — Ratio | (Total % w/w of carnosic acid and carnosol) ≥ 15 (% w/w of reference key volatiles)* (* as a percentage of total volatiles in the extract, measured by Gas Chromatography — Mass Spectrometry Detection, ‘GC-MSD’) |
| Purity | |
| Residual solvents | Acetone: Not more than 500 mg/kg |
2 — Extracts of rosemary prepared by extraction of dried rosemary leaves by means of supercritical carbon dioxide.
| Description | Extracts of rosemary produced from dried rosemary leaves extracted by means of supercritical carbon dioxide with a small amount of ethanol as entrainer. |
| Identification | |
|---|---|
| Content of reference antioxidative compounds | ≥ 13 % w/w, expressed as the total of carnosic acid and carnosol |
| Antioxidant/Volatiles – Ratio | (Total % w/w of carnosic acid and carnosol) ≥ 15 (% w/w of reference key volatiles)* (* as a percentage of total volatiles in the extract, measured by Gas Chromatography — Mass Spectrometry Detection, ‘GC-MSD’) |
| Purity | |
| Residual solvents | Ethanol: not more than 2 % |
3 — Extracts of rosemary prepared from a deodorised ethanolic extract of rosemary.
| Description | Extracts of rosemary which are prepared from a deodorised ethanolic extract of rosemary. The extracts may be further purified, for example by treatment with active carbon and/or molecular distillation. The extracts may be suspended in suitable and approved carriers or spray dried. |
| Identification | |
|---|---|
| Content of reference antioxidative compounds | ≥ 5 % w/w, expressed as the total of carnosic acid and carnosol |
| Antioxidant/Volatiles – Ratio | (Total % w/w of carnosic acid and carnosol) ≥ 15 (% w/w of reference key volatiles)* (* as a percentage of total volatiles in the extract, measured by Gas Chromatography – Mass Spectrometry Detection, ‘GC-MSD’) |
| Purity | |
| Residual solvents | Ethanol: not more than 500 mg/kg |
4 — Extracts of rosemary decolourised and deodorised, obtained by a two-step extraction using hexane and ethanol.
| Description | Extracts of rosemary which are prepared from a deodorised ethanolic extract of rosemary, undergone a hexane extraction. The extract may be further purified, for example by treatment with active carbon and/or molecular distillation. They may be suspended in suitable and approved carriers or spray dried. |
| Identification | |
|---|---|
| Content of reference antioxidative compounds | ≥ 5 % w/w, expressed as the total of carnosic acid and carnosol |
| Antioxidant/Volatiles – Ratio | (Total % w/w of carnosic acid and carnosol) ≥ 15 (% w/w of reference key volatiles)* (* as a percentage of total volatiles in the extract, measured by Gas Chromatography – Mass Spectrometry Detection, ‘GC-MSD’) |
| Purity | |
| Residual solvents | Hexane: not more than 25 mg/kg Ethanol: not more than 500 mg/kg |
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