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6. Take 25 ml of the extract solution obtained by Method 25a or Method 25b and place in a 100 ml beaker. In the case of Method 25b add 5 ml of the dilute hydrochloric acid solution (4.1). Then add 5 ml of the hydrogen peroxide solution (4.2). Cover with a watch glass. Allow oxidation to occur at room temperature for about one hour, then bring gradually to boiling and boil for half an hour. If necessary, add a further 5 ml of the hydrogen peroxide to the solution once it has cooled. Then boil to remove the excess hydrogen peroxide. Allow to cool and transfer quantitatively to a 50 ml volumetric flask and make up to volume. Filter where necessary.
Account should be taken of this dilution when taking aliquot portions and calculating the amount of trace element in the product.
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