The Poultry Breeding Flocks and Hatcheries Order 1993

Article 3(4)

SCHEDULE 1

PART I PARTICULARS TO BE NOTIFIED TO THE APPROPRIATE MINISTERFOR THE PURPOSES OF ARTICLE 3(4)

(i) The name, address and telephone number of the applicant, including the names of partners if a partnership and the registered number if a company.

(ii) The address and telephone number of the holding on which the flock is to be kept.

(iii) The name of the person in charge of the holding on which the flock is to be kept (if not the applicant).

(iv) The species of birds in the flock.

(v) The approximate number of birds in the flock.

Article 4(4)

PART II PARTICULARS TO BE NOTIFIED TO THE APPROPRIATE MINISTERFOR THE PURPOSES OF ARTICLE 4(4)

(i) The name, address and telephone number of the applicant, including the names of partners if a partnership, and the registered number if a company.

(ii) The address and telephone number of the premises which are to be used as a hatchery.

(iii) The name of the person in charge of the premises which are to be used as a hatchery (if not the applicant).

(iv) The incubator capacity of the premises which are to be used as a hatchery and the species of birds to be hatched there.

Articles 2(1) and 5(a)

SCHEDULE 2

PART I SAMPLES TO BE TAKEN FROM BREEDING FLOCKS OF DOMESTIC FOWL FOR BACTERIOLOGICAL TESTING

1.  The number of sites from which separate faeces samples are to be taken in order to make a composite sample shall be as follows:

Number of birds kept in a building, or on a holding on which birds have free access to more than one building, number of birds in each group of buildings on such holding	Number of faeces samples to be taken in the building or group of buildings on the holding
1—24	A number equal to the total number of birds up to a maximum of 20 birds
25—29	20
30—39	25
40—49	30
50—59	35
60—89	40
90—199	50
200—499	55
500 or more	60

2.  The samples to be taken shall comprise—

(a) one chick box liner, up to a maximum of 10, for every 500 chicks delivered from each hatchery to any rearing premises on any day, such samples to be taken on the day of the arrival of the chicks there;

(b) the carcases of all chicks, up to a maximum of 60, from each hatchery, which are dead on arrival at any rearing premises, such samples to be taken on the day of the arrival of the chicks there;

(c) a composite faeces sample taken from birds at 4 weeks of age and at two weeks prior to them entering the laying phase, the number of separate samples being taken in accordance with the table in paragraph 1 above;

(d) in the case of birds whose eggs are hatched at a hatchery with a total incubator capacity of less than 1,000 eggs a composite faeces sample taken from birds at the time they enter the laying phase and at intervals of 2 weeks thereafter in accordance with the table in paragraph 1 above.

3.  Samples referred to in subparagraph (d) above shall be taken under the supervision of an officer of the Minister at 56 day intervals.

Article 6(a)

PART II SAMPLES TO BE TAKEN FROM FLOCKS OF DOMESTIC FOWL AT HATCHERIES FOR BACTERIOLOGICAL TESTING

1.  The samples to be taken shall comprise—

(a) a composite sample of meconium taken from 250 chicks, one such sample being taken every seven days in the case of grandparent flocks and every 14 days in the case of parent flocks, from those chicks hatched from eggs supplied to the hatchery from any particular breeding flock (including flocks of less than 250 domestic fowl); or

(b) samples comprising—

(i) the carcases of all chicks which are dead in the shells of eggs supplied to the hatchery from any particular breeding flock, and

(ii) the carcases of all chicks hatched from eggs supplied to the hatchery from any particular breeding flock and which have been culled,

such samples being taken from grandparent flocks every 7 days and from parent flocks every 14 days, up to a maximum of 50 in total.

2.  Samples taken for the purposes of paragraph 1 above shall be taken, every 28 days for grandparent flocks and every 56 days for parent flocks, under the supervision of an officer of the Minister.

Article 5(c), 6(c) and 7(1)(c)

PART III BACTERIOLOGICAL METHODS FOR TESTING FOR SALMONELLA

1.  Bacteriological method (Rappaports) for the detection of salmonella in chick box liners, composite faeces samples, meconium samples and carcases.

Samples submitted for testing for the presence of salmonella shall be examined in the following prescribed manner on consecutive days and, where a laboratory at which samples have been received for testing on any day is unable to commence such an examination on that day, the samples shall be stored in a refrigerator at between 1 degree C and 4 degrees C until required for examination.

• Day 1

  (a)

  Chick box liners: a one gram portion shall be taken from a soiled area on each liner and the portions from separate liners shall be bulked together and placed in Buffered Peptone Water (BPW)(a), at the rate of 1 gram of liner in 10 ml of BPW up to a maximum of 10 grams in 100 ml of BPW.

  (b)

  Composite faeces and meconium samples: the samples shall be thoroughly mixed and a sub-sample weighing not more than 10 grams shall be placed in BPW at the rate of 1 gm sample to 10 ml BPW to a maximum of 10 grams in 100 ml BPW.

  (c)

  Carcases of chicks: there shall be removed samples of the yolk sac, liver and terminal intestines to include portions of small intestines, large intestine and caecal tonsil.The samples of organs taken from the carcases of chicks submitted shall then be bulked together and placed in BPW at the rate of 1 gram of bulked tissue in 10 ml BPW up to a maximum of 10 grams of tissue in 100 ml BPW.

  The inoculated BPW shall then be incubated at 37°C for 18-24 hours.

• Day 2

  0.1 ml from the incubated BPW shall be inoculated into 10 ml of Rappaports Vassiliadis (RV) broth or Rappaports Vassiliadis Soya Peptone (RVS)(b) broth and incubated at 41.5 degrees C ± 0.5° C for 18-24 hours.

• Day 3

  The RV or RVS broth shall be plated out on to two plates of Brilliant Green Agar (BGA)(c) using a 10 microlitres loop. The BGA plates shall be inoculated with a droplet taken from the edge of the surface of the fluid and drawing the loop over the whole of one plate in a zigzag pattern and continuing to the second plate without recharging the loop. The space between the loop streaks shall be 0.5-1.0 cm. The plates shall be incubated at 37 degrees C for 18-24 hours, and the RV or RVS broth reincubated at 41.5 degrees C ± 0.5° C for a further 18-24 hours.

• Day 4

  (i)

  The plates of BGA shall be examined and a minimum of 3 colonies from the plates showing suspicion of salmonella growth shall be subcultured on to a blood agar plate and a MacConkey agar plate and into biochemical composite media or equivalent.These media shall be incubated at 37 degrees C for 18-24 hours.

  (ii)

  The reincubated RV or RVS broth shall be plated out, and the plates incubated, as described in Day 3.

• Day 5

  (i)

  The incubated plates and composite media or equivalent shall be examined and the findings recorded, discarding cultures which are obviously not salmonella. Slide serological tests shall be performed using salmonella polyvalent “O”(Groups A-S) and polyvalent “H” (phase 1 and 2) agglutinating sera on selected suspect colonies collected from the blood agar or MacConkey plates.If reactions occur with one or both sera, the colonies shall be typed to Group level by slide serology.

  (ii)

  The plates of BGA prepared at Day 4(ii) shall be examined and further action taken as described in Day 4(i) and Day 5(i).

2.  Bacteriological method (Selenite) for the detection of salmonella in chick box liners, composite faeces samples, meconium samples and carcases.

Samples submitted for testing for the presence of salmonella shall be examined in the following prescribed manner on consecutive days and, where a laboratory at which samples have been received for testing on any day is unable to commence such an examination on that day, the samples shall be stored in a refrigerator at between 1 degree C and 4degrees C until required for examination.

• Day 1

  (a)

  Chick box liners: a one gram portion shall be taken from a soiled area on each liner and the portions from separate liners shall be bulked together and placed in Selenite F broth(d) at the rate of 1 gram of liner to 10 ml broth up to a maximum of 10 grams of liner in 100 ml broth.

  (b)

  Composite faeces and meconium samples: the sample shall be thoroughly mixed and a sub-sample weighing not more than 10 grams shall be placed in Selenite F broth at the rate of 1 gram of faeces to 10 ml broth up to a maximum of 10 grams of faeces in 100 ml broth.

  (c)

  Carcases of chicks: there shall be removed samples of the yolk sac, liver and terminal intestines (to include portions of small intestines, large intestine and caecal tonsil).

  The samples of organs taken from the carcases of chicks submitted shall then be bulked together and placed in Selenite F broth at the rate of 1 gram of bulked tissue in 10 ml of broth up to a maximum of 10 grams of tissue in 100 ml broth.

  The inoculated Selenite F broth shall then be incubated at 37°C for 18-24 hours.

• Day 2

  (i)

  The Selenite F broth shall be plated out on to two plates of Brilliant Green Agar (BGA) using a 10 microlitres loop. The BGA plates shall be inoculated with a droplet taken from the edge of the surface of the fluid and drawing the loop over the whole of one plate in a zigzag pattern and continuing to the second plate without recharging the loop. The space between the loopstreaks shall be 0.5 cm-1.0 cm.The plates shall be incubated at 37 degrees for 18-24 hours.

  (ii)

  The Selenite F broth shall then be reincubated at 37 degrees C for a further 18-24 hours.

• Day 3

  (i)

  The plates of BGA shall be examined and a minimum of 3 colonies from the plates showing suspicion of salmonella growth shall be subcultured on to a blood agar plate and a MacConkey agar plate and into biochemical composite media or equivalent. These media shall be incubated at 37 degrees C for 18-24 hours.

  (ii)

  The reincubated Selenite F broth shall be plated out and incubated asdescribed in Day 2(i).

• Day 4

  (i)

  The incubated plates and composite media or equivalent shall be examined and the findings recorded, discarding cultures which are obviously not salmonella. Slide serological tests shall be performed using salmonella polyvalent “O”(Groups A-S) and polyvalent “H” (phase 1 and 2) agglutinating sera on selected suspect colonies collected from the blood agar or MacConkey plates. If reactions occur with one or both sera, the colonies shall be typed to Group level by slide serology.

  (ii)

  The plates of BGA prepared at Day 3(ii) shall be examined and further action taken as described in Day 3(i)and Day 4(i).

(a)

Buffered Peptone Water-Edel and Kampelmacher (1973) (commercially available as Oxoid CM 509, Lab M46 or equivalent).

(b)

Rappaports Vassiliadis (RV) Broth-Vassiliadis et al. (1976) (commercially available as Oxoid CM 669 or equivalent) or Rappaports Vassiliadis Soya Peptone (RVS) broth (Oxoid CM 866).

(c)

Brilliant Green Agar (Modified)-Edel and Kampelmacher (1969) (commercially available as Oxoid CM 329, Lab M34 or equivalent).

(d)

Selenite F broth-Liefson (1936) (commercially available as Oxoid CM 395 and L121, Lab M44a and 44b or equivalent).

(a), (b), (c) and (d) should be reconstituted according to the manufacturer’s instructions and in the case of (c) poured into 9 cm diameter plates.

References for the above substances are found in:

• Liefson E. (1936) American Journal of Hygiene 24, 423-432.

• Edel, W. & Kampelmacher, E.H. (1969) Bulletin of the World Organisation 41, 297-306.

• Edel, W. & Kampelmacher, E.H. (1973) Bulletin of the World Health Organisation 48, 167-174.

• Anon (1969) ISO 6579 International Organisation for Standardisation, Geneva. Vassiliadis, P., Pateraki, E., Papaiconomou, N., Papadakis, J.A., and Trichopoulos, D. (1976) Annales de Microbiologie (Institut Pasteur) 127B, 195-200.

Article 9(a)

SCHEDULE 3

PART I RECORDS OF SAMPLES TAKEN IN RESPECT OF A BREEDING FLOCK OR A HATCHERY

A person whose name is entered in the Breeding Flocks Register in respect of any holding on which a breeding flock of domestic fowl is kept by him or whose name is entered in the Hatcheries Register in respect of any premises used by him as a hatchery of domestic fowl shall keep a record of samples taken in respect of the flock or hatchery (as the case may be) containing the following information—

(i) the date on which the sample was taken;

(ii) a description of the type of sample taken; and

(iii) in the case of samples taken in respect of a breeding flock, the identity of the building or group of buildings from which the samples were taken and, in the case of samples taken from a hatchery, the address of the holding from which the hatching eggs from which the samples were obtained were supplied to the hatchery.

Article 9(c)

PART II RECORDS OF THE MOVEMENT OF POULTRY, CHICKS AND EGGS ONTO AND OFF ANY HOLDING ON WHICH A BREEDING FLOCK IS KEPT

A person whose name is entered in the Breeding Flocks Register in respect of any holding on which a breeding flock of domestic fowl is kept shall keep a record of the movement of any domestic fowl, their chicks and eggs onto and off such holding which record shall contain the following information—

(i) the date of the movement;

(ii) the number of domestic fowl, their chicks or eggs moved;

(iii) the identity of the building or group of buildings in which any domestic fowl, their chicks or eggs moved onto the holding were placed or from which any domestic fowl, their chicks or eggs were moved off the holding;

(iv) in the case of any domestic fowl, their chicks or eggs moved onto the holding, the address from which they were brought there; and

(v) in the case of any domestic fowl, their chicks or eggs moved off the holding, the address to which they were moved.

Article 9(d)

PART III RECORDS OF THE MOVEMENT OF EGGS ONTO AND OFF, AND OF THE MOVEMENT OF CHICKS OFF, ANY PREMISES WHICH ARE USED AS A HATCHERY

A person whose name is entered in the Hatcheries Register in respect of any premises used by him as a hatchery shall keep a record of the movement of any eggs of domestic fowl onto and off, and of the movement of any chicks of domestic fowl off, such premises, which record shall contain the following information—

(i) the date of the movement;

(ii) in the case of the movement of any eggs onto the premises, the address of the premises from which they were moved and the number of eggs moved;

(iii) in the case of the movement of any eggs or chicks off the premises the address to which they were moved and the number of eggs or chicks moved.