1. Introductory Text

  2. Article 1.Without prejudice to the Community provisions governing intra-Community trade, this...

  3. Article 2.For the purpose of this Directive, the definitions given in...

  4. Article 3.Member States shall ensure that there is compulsory and immediate...

  5. Article 4.(1) When poultry in a holding are suspected of being...

  6. Article 5.(1) Once the presence of Newcastle disease in poultry has...

  7. Article 6.In the case of holdings which consist of two or...

  8. Article 7.(1) The epizootiological inquiry shall deal with: the length of...

  9. Article 8.(1) Where the official veterinarian has reason to suspect that...

  10. Article 9.(1) Once the diagnosis of Newcastle disease has been officially...

  11. Article 10.Member States shall ensure that: the competent authority determines the...

  12. Article 11.Member States shall ensure that: the disinfectants to be used...

  13. Article 12.Collection of samples and laboratory testing to detect the presence...

  14. Article 13.Member States shall ensure that the competent authority takes all...

  15. Article 14.(1) Member States shall ensure that, in each Member State...

  16. Article 15.(1) The Commission shall, by means of implementing acts, designate...

  17. Article 16.(1) Member States shall ensure that: (a) vaccination against Newcastle...

  18. Article 17.(1) A Member State in which voluntary or compulsory prophylactic...

  19. Article 18.(1) Member States shall ensure that, when Newcastle disease has...

  20. Article 19.(1) Where carrier pigeons or birds kept in captivity are...

  21. Article 20.(1) The use of swill originating from means of international...

  22. Article 21.(1) Each Member State shall draw up a contingency plan,...

  23. Article 22.Commission experts may, in collaboration with the competent authorities, and...

  24. Article 23.The detailed conditions governing the Community's financial contribution to the...

  25. Article 24.The Annexes shall be amended, as and when required, by...

  26. Article 25.(1) The Commission shall be assisted by the Standing Committee...

  27. Article 26.(1) Member States shall bring into force the laws, regulations...

  28. Article 27.This Directive is addressed to the Member States.

    1. ANNEX I

      AUTHORIZATION TO REMOVE EGGS FROM A HOLDING SUBJECT TO THE CONDITIONS OF ARTICLE 4 (2) (e) OF THIS DIRECTIVE

      1. The authorization issued by the competent authority to transport eggs...

      2. in order to be allowed to be removed from a...

    2. ANNEX II

      PROCEDURE FOR CLEANING AND DISINFECTING AN INFECTED HOLDING

      1. I. Preliminary cleaning and disinfecting

      2. II. Final cleaning and disinfection

    3. ANNEX III

      DIAGNOSTIC PROCEDURES FOR THE CONFIRMATION AND DIFFERENTIAL DIAGNOSIS OF NEWCASTLE DISEASE

      1. The following procedures for the isolation and characterization of Newcastle-disease...

      2. The virus responsible for Newcastle disease is the prototype virus...

      3. ‘Newcastle disease’ means an infection of poultry caused by any...

      4. CHAPTER 1 Sampling and treatment of samples

        1. 1. Samples

        2. 2. Treatment of samples

        3. 3. Antibiotic medium

      5. CHAPTER 2 Virus isolation

        1. Virus isolation in embryonated fowls' eggs

      6. CHAPTER 3 Differential diagnosis

        1. 1. Preliminary differentiation

        2. 2. Confirmatory identification

        3. 3. Further typing and characterization of isolates

      7. CHAPTER 4 Rapid tests for detection of Newcastle-disease virus and antibodies

        1. 1. Detection of Newcastle disease virus

        2. 2. Detection of antibodies in unvaccinated birds

          1. (a) Samples

          2. (b) Examination for antibodies

      8. CHAPTER 5 Haemagglutination (HA) test

        1. Reagents

          1. 1. Isotonic saline buffered with phosphate (PBS) (0,05 M) to pH...

          2. 2. Red blood cells (RBC) taken and pooled from a minimum...

          3. 3. NDV strain Ulster 2C is recommended for use as standard...

        2. Procedure

      9. CHAPTER 6 Haemagglutination inhibition (HI) test

        1. Reagents (see Chapter 5)

        2. Procedure

      10. CHAPTER 7 Intracerebral pathogenicity index test

        1. 1. Infective freshly harvested allantoic fluid (HA titre must be greater...

        2. 2. 0,05 ml of the diluted virus is injected intracerebrally into...

        3. 3. The birds are examined at intervals of 24 hours for...

        4. 4. At each observation each bird is scored: 0 = normal;...

        5. 5. The index is calculated as shown in the following example:...

      11. CHAPTER 8 Assessment of plaque-forming ability

        1. 1. It is usually best to use a dilution range of...

        2. 2. Confluent monolayers of chick embryo cells or a suitable cell...

        3. 3. 0,2 ml of each virus dilution is added to each...

        4. 4. After washing three times with PBS the infected cells are...

        5. 5. After 72 hours, incubation at 37 °C the plaques should...

        6. 6. All viruses should give clear plaques when incubated in the...

    4. ANNEX IV

      LIST OF NATIONAL NEWCASTLE DISEASE LABORATORIES

    5. ANNEX V

      COMMUNITY REFERENCE LABORATORY FOR NEWCASTLE DISEASE

      1. Name of Laboratory:

      2. . . . . . . . . . ....

      3. . . . . . . . . . ....

    6. ANNEX VI

      1. . . . . . . . . . ....

    7. ANNEX VII

      MINIMUM CRITERIA FOR CONTIGENCY PLANS

      1. . . . . . . . . . ....

      2. . . . . . . . . . ....