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Article 1.(1) Oils, the characteristics of which comply with those set...
Article 2.(1) The characteristics of oils laid down in Annex I...
Article 2a.(1) For the purpose of this Article, ‘ olive oil...
Article 3.Where it is found that an oil does not correspond...
Article 4.(1) The Member States may approve assessment panels so that...
Article 6.(1) The oil content of oil cake and other residues...
Article 7.The Community provisions concerning the presence of contaminants shall apply....
Article 7a.Natural or legal persons and groups of persons who hold...
Article 8.(1) Member States shall notify the Commission of the measures...
Article 10.(1) This Regulation shall enter into force on the third...
SAMPLING OF OLIVE OIL OR OLIVE-POMACE OIL DELIVERED IN IMMEDIATE PACKAGING
FLOW-CHART FOR VERIFYING WHETHER AN OLIVE OIL SAMPLE IS CONSISTENT WITH THE CATEGORY DECLARED
DETERMINATION OF PEROXIDE VALUE
5.1. Chloroform, analytical reagent quality, freed from oxygen by bubbling a...
5.2. Glacial acetic acid, analytical reagent quality, freed from oxygen by...
5.3. Potassium iodide, saturated aqueous solution, recently prepared, free from iodine...
5.4. Sodium thiosulphate, 0,01 mol/l (equivalent to 0,01 N) accurately standardised...
5.5. Starch solution, 10 g/l aqueous dispersion, recently prepared from natural...
DETERMINATION OF WAX CONTENT BY CAPILLARY COLUMN GAS CHROMATOGRAPHY
3.2. Glass column for gas chromatography, internal diameter 15,0 mm, length...
3.3. Suitable gas chromatograph with a capillary column, equipped with a...
3.4. 10 μl microsyringe for on-column injection, equipped with a hardened...
3.8. Analytical balance with guaranteed precision of ± 0,1 mg.
DETERMINATION OF ERYTHRODIOL AND UVAOL
DETERMINATION OF THE PERCENTAGE OF 2-GLYCERYL MONOPALMITATE
3.3. Glass chromatograph column, internal diameter 21-23 mm, length 400 mm,...
3.7. 10 ml conical-bottomed centrifuge tubes with groundglass stopper
3.9. Thermostat permitting a stable temperature of 40 ± 0,5 °C...
3.14. Capillary gas chromatograph with an on-column cold injector for direct...
3.15. On-column cold injector for direct injection of the sample into...
3.17. Recorder-integrator adapted to the electrometer with a response rate no...
3.18. Capillary column made of glass or fused silica 8-12 metres...
3.19. 10 μl microsyringe fitted with a hardened needle, at least...
4.2. n-hexane (chromatography grade). Hexane may be replaced by iso-octane (2,2,4-...
4.5. Pancreatic lipase. It must have an activity of between 2,0...
4.6. Buffer solution of trishydroxymethylaminomethane: 1 M aqueous solution adjusted to...
4.7. Enzyme-quality sodium cholate, 0,1 % aqueous solution (this solution must...
4.10. Developer solvent: mixture of n-hexane/diethyl ether (87:13 v:v)
4.13. Carrier gas: hydrogen or helium, for gas chromatography
4.14. Auxiliary gases: hydrogen, 99 % minimum purity, free from moisture...
4.16. Reference samples: pure monoglycerides or monoglyceride mixtures with a known...
DETERMINATION OF TRILINOLEIN CONTENT
DETERMINATION OF FATTY ACID METHYL ESTERS BY GAS CHROMATOGRAPHY
PART A PREPARATION OF THE FATTY ACID METHYL ESTERS FROM OLIVE OIL...
3.1. Trans-esterification with methanolic solution of potassium hydroxide at room temperature...
3.1.2.1. Methanol containing not more than 0,5 % (m/m) water.
3.1.2.6. Elution solvent for purifying the oil by column/SPE chromatography, mixture...
3.1.2.7. Potassium hydroxide, approximately 2M methanolic solution: dissolve 11,2 g of...
3.1.2.8. Silica gel cartridges, 1 g (6 ml), for solid phase...
PART B ANALYSIS OF FATTY ACID METHYL ESTERS BY GAS CHROMATOGRAPHY
DETERMINATION OF VOLATILE HALOGENATED SOLVENTS CONTENT OF OLIVE OIL
THE INTERNATIONAL OLIVE COUNCIL’S METHOD FOR THE ORGANOLEPTIC ASSESSMENT OF VIRGIN OLIVE OIL
9. PROCEDURE FOR THE ORGANOLEPTIC ASSESSMENT AND CLASSIFICATION OF VIRGIN OLIVE...
Method for calculating the median and the confidence intervals
(1) Wilkinson, L. 1990. Systat: The system for statistics. Evanston, IL.SYSTAT...
(2) Cicchitelli, G. 1984. Probabilità e Statistica. Maggioli Editore, Rimini.
(3) Massart, D.L.; Vandeginste, B.G.M.; Deming, Y.; Michotte, L. 1988. Chemometrics....
(4) Kendall, M.G.; Stuart, A. 1967. The advanced theory of statistics....
(5) McGill, R.; Tukey, J.W.; Larsen, W.A. 1978. Variation of Box...
(6) IOC/T.28/Doc. No 1 September 2007, Guidelines for the accreditation of...
NEUTRALIZATION AND DECOLORIZATION OF OLIVE OIL IN THE LABORATORY
ADDITIONAL NOTES 2, 3 AND 4 TO CHAPTER 15 OF THE COMBINED NOMENCLATURE
4.1. water, complying with the requirements of ISO 3696, Grade 3....
4.2. potassium iodide, 100 g/l solution, not containing iodate or free...
4.4. sodium thiosulfate, standard volumetric solution c (Na2S2O3.5H2O) = 0,1 mol/l,...
4.5. solvent, prepared by mixing equal volumes of cyclohexane and acetic...
4.6. Wijs reagent, containing iodine monochloride in acetic acid. Commercially available...
METHOD FOR THE DETERMINATION OF STIGMASTADIENES IN VEGETABLE OILS
4.1. 250 ml flasks suitable for use with a reflux condenser....
4.5. Glass chromatography column (1,5 to 2,0 cm internal diameter by...
4.6. Gas chromatograph with flame ionization detector, split or cold on-column...
4.7. Fused silica capillary column for gas chromatography (0,25 or 0,32...
4.8. Integrator-recorder with possibility of valley-valley integration mode.
4.9. 5 to 10 ml microsyringe for gas chromatography with cemented...
5.1. Hexane or mixture of alkanes of b.p. interval 65 to 70 °C,...
5.4. Alcoholic potassium hydroxide solution at 10 %. Add 10 ml...
5.5. Silica gel 60 for column chromatography, 70 to 230 mesh,...
5.6. Stock solution (200 ppm) of cholesta-3,5-diene (Sigma, 99 % purity)...
5.7. Standard solution of cholesta-3,5-diene hexane at concentration of 20 ppm,...
5.8. Solution of n-nonacosane in hexane at concentration of approximately 100...
5.9. Carrier gas for chromatography: helium or hydrogen of 99,9990 %...
5.10. Auxiliary gases for flame ionization detector: hydrogen of 99,9990 %...
Gas chromatogram obtained from a refined olive oil sample analysed...
4.1.3. Glass chromatographic column, 21 mm internal diameter, 450 mm length,...
4.1.4. Separating funnels, 250 ml, with normalised cone (male) at the...
4.1.12. Detector: differential refractometer. The full scale sensitivity should be at...
4.1.13. Column: stainless steel tube 250 mm length x 4,5 mm...
4.1.15. Vials, of about 2 ml volumes, with Teflon-layered septa and...
4.2.1. Petroleum ether 40-60 °C chromatographic grade or hexane. Hexane may be...
4.2.3. Elution solvent for purifying the oil by column chromatography mixture...
4.2.4. Silica gel, 70-230 mesh, type Merck 7734, with water content...
4.2.8. HPLC elution solvent: acetonitrile + acetone (proportions to be adjusted...
4.2.11. Solid phase extraction column with silica phase 1 g, 6...
4.2.12. Heptane, chromatographic quality. Heptane may be replaced by iso-octane (2,2,4-trimethyl...
4.5. Calculation of triacylglycerols composition (moles %) from fatty acid composition...
6. EXAMPLE (THE NUMBERS REFER TO THE SECTIONS IN THE TEXT...
DETERMINATION OF THE STEROL COMPOSITION AND CONTENT AND ALCOHOLIC COMPOUNDS BY...
PART 3 GAS CHROMATOGRAPHIC ANALYSIS OF THE ALCOHOLIC COMPOUNDS FRACTIONS
Figure 2 — GC-FID chromatographic profile of the sterol and...
Figure 3 — GC-FID chromatographic profile of the sterol and...
Figure 4 — GC-FID chromatographic profile of aliphatic alcohols and...
Figure 5 — GC-FID chromatographic profile of aliphatic alcohols and...
Figure 6 — HPLC Chromatogram of an olive oil unsaponifiable...
3.2. Glass column for liquid chromatography, internal diameter 15 mm, length...
3.3. Gas chromatograph suitable for use with a capillary column, equipped...
3.4. Microsyringe , 10 μl, with hardened needle, for direct on-column...
3.8. Analytical balance for weighing to an accuracy of ± 0,1...
METHOD FOR THE DETECTION OF EXTRANEOUS OILS IN OLIVE OILS